HepG-2 liver cancer cells were purchased from ATCC (Manassas, VA 20110, USA) and Hep-3B liver cancer cells from Sigma (St. Louis, MO, USA). Patient-Derived Tumor (PDT), human colorectal cancer (hCRC) organoids line 18SH112T was obtained from the Hudson-Monash Cancer Center, (Melbourne, Australia) where all organoid-based studies are performed under proper authorization.
Hepg2 liver cancer cells
Manufactured by American Type Culture Collection
Sourced in United States
HepG2 liver cancer cells are a commonly used human liver cancer cell line. They are derived from a 15-year-old Caucasian male with a well-differentiated hepatocellular carcinoma. HepG2 cells maintain several liver-specific functions and are widely utilized in liver-related research and applications.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Corning (1 mentions)
Fetal bovine serum (fbs), by Omega Scientific (1 mentions)
Mda mb 231 breast cancer cells, by American Type Culture Collection (1 mentions)
Skov 3 ovarian cancer cells, by American Type Culture Collection (1 mentions)
Hct116 colon cancer cells, by American Type Culture Collection (1 mentions)
Skbr3 breast cancer cells, by American Type Culture Collection (1 mentions)
Hek293t cell, by American Type Culture Collection (1 mentions)
Hacat cell line, by American Type Culture Collection (1 mentions)
Trisodium citrate, by Merck Group (1 mentions)
Hydrogen tetrachloroaurate, by Merck Group (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Co2 incubator, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Greiner (1 mentions)
Hanks balanced salt solution (hbss), by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions)
Fluorescein disodium salt, by Merck Group (1 mentions)
7 protocols using hepg2 liver cancer cells
1
Culturing liver cancer and CRC organoids
2
Cell Culture Protocols for Cancer Research
3
Liver and Colon Cancer Cell Lines
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Normal human liver cells line LO2, and liver cancer cell lines, QGY-7703, Bel-7402 and SMMC-7721 were from cell bank at Shanghai Cell Biology Institute of Chinese Academy of Sciences, while HepG2 liver cancer cells, SW480 and SW620 colon cancer cells, and HEK293T cells were from American Type Culture Collection (ATCC). Stable cell line HepG2/RARγ with RARγ overexpression was established by transfecting neo-RARγ and selected by 0.5 mg/ml G418.
4
Culturing HepG2 and Chang Liver Cells
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The HepG2 liver cancer cells and Chang normal liver cells were purchased from American Type Culture Collection (ATCC, USA) and cultured in DMEM supplemented with 10% FBS, 100 µg/mL streptomycin and 100 units/mL penicillin and maintained at 37 °C with 5% CO2 in humidified atmosphere.
5
Gold Nanoparticle Synthesis and Characterization
6
Atractylon Dose-Response in HepG2 Cells
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HepG2 liver cancer cells were purchased from the American Type Culture Collection (ATCC). By STR identification, cell line was authenticated. The cells were incubated in DMEM (Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (FBS; Greiner Bio-One International GmbH) and 1% penicillin/streptomycin in the 5% CO2 incubator (Thermo Fisher Scientific, Inc.). Cells were passaged three times, then collected by trypsinization and centrifugation at 400 × g and 4°C for 5 min, and separated into three experimental groups (treated with 5 µM, 10 µM or 20 µM atractylon) and a control group. Cells were resuspended in serum-free cell culture medium.
7
Antioxidant Assay Protocol for HepG2 Cells
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Ascorbic acid, 29, 79-dichlorofluorescin diaacetate (DCFH-DA), fluorescein disodium salt, sodium borohydride (NaBH4, reagent grade), chloranil (analytical grade), vanillin (analytical grade), quercetin dehydrated, catechin hydrated, 5, 7, were purchased from Sigma-Aldrich, Inc. (St. Louis, MO). HepG2 liver cancer cells were obtained from the American Type Culture Collection (ATCC) (Rockville, MD). Williams' Medium E (WME) and Hanks' Balanced Salt Solution (HBSS) were purchased from Gibco Life Technologies (Grand Island, NY). Fetal bovine serum (FBS) was obtained from Atlanta Biologicals (Lawrenceville, GA).
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