Anti-LC3B, anti-Bcl-xL, anti-Atg16L1, anti-Rac1, anti-Rho1, anti-Cdc42, anti-phospho-ERK, anti-EEA1 and anti-LAMP1 antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Mouse monoclonal antibodies reactive with LRP1 (11H4) were a kind gift from Dr. Strickland, University of Maryland School of Medicine, Baltimore. Fibronectin (EP5) and ubiquitin (P4D1) were from Santa Cruz Biotechnologies (Santa Cruz, CA, USA); anti-Cx43, anti-ERK, anti-Bcl-2 and anti-RPTPβ antibodies were from BD Biosciences (Tokyo, Japan); anti-multi ubiquitin monoclonal antibody (FK1) was from MBL (Nagoya, Japan); anti-GAPDH antibody was from GeneTex (Irvine, CA, USA) and anti-LC3 (clone 1703) antibody was from Cosmo Bio (Tokyo, Japan). Anti-RPTPα rabbit polyclonal antibodies for immunoblotting were provided by Dr. Jan Sap; anti-α-tubulin and anti-FLAG M2 antibodies, N-acetyl-l -cysteine (NAC) and ammonium chloride were from Sigma Aldrich (St. Louis, MO, USA); ProLong Gold Antifade Reagent with DAPI and DIDS were from Invitrogen (Eugene, OR, USA); an ERK inhibitor, U0126, was from Cayman Chemical (Ann Arbor, MI, USA); Rac1 inhibitor, NSC23766 was from Wako Pure Chemical Industries (Osaka, Japan); and GSH was from Merck (Darmstadt, Germany).
Anti cx43
Manufactured by BD
Sourced in United States
Anti-Cx43 is a laboratory reagent used for the detection and analysis of the protein Connexin 43 (Cx43) in biological samples. Cx43 is a key component of gap junctions, which facilitate intercellular communication. Anti-Cx43 can be used in various research applications, such as immunohistochemistry, Western blotting, and cell biology studies.
Automatically generated - may contain errors
Lab products found in correlation
Anti s100a4, by Abcam (2 mentions)
Anti eea1, by Cell Signaling Technology (1 mentions)
Anti cdc42, by Cell Signaling Technology (1 mentions)
N acetyl l cysteine nac, by Merck Group (1 mentions)
Anti atg16l1, by Cell Signaling Technology (1 mentions)
Anti rac1, by Cell Signaling Technology (1 mentions)
Anti lamp1, by Cell Signaling Technology (1 mentions)
Fibronectin ep5, by Santa Cruz Biotechnology (1 mentions)
Ubiquitin p4d1, by Santa Cruz Biotechnology (1 mentions)
Anti erk, by BD (1 mentions)
Anti bcl 2, by BD (1 mentions)
Anti rptpβ, by BD (1 mentions)
Anti gapdh antibody, by GeneTex (1 mentions)
Anti α tubulin and anti flag m2 antibodies, by Merck Group (1 mentions)
Glutathione (gsh), by Merck Group (1 mentions)
U0126, by Cayman Chemical (1 mentions)
Anti phospho erk, by Cell Signaling Technology (1 mentions)
Prolong gold antifade reagent with dapi, by Thermo Fisher Scientific (1 mentions)
Anti lc3b, by Cell Signaling Technology (1 mentions)
Anti bcl xl, by Cell Signaling Technology (1 mentions)
3 protocols using anti cx43
1
Antibody Reagents for Cell Signaling
2
Protein Expression Profiling by Western Blot
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Extracted protein were loaded into polyacrylamide gels and transferred onto PVDF membranes. The membranes were blocked in 5% nonfat milk containing 0.3% tween 20, and then probed with anti-Cx43 (BD Transduction Labs), anti-fibronectin (BD Transduction Labs), anti-β-actin (sigma), anti-GAPDH (Abcam), anti-α-SMA (Abcam) p-smad2/3 (Abcam), TGFβR2 (Abcam) or anti-S100A4 (Abcam) antibody at 4°C overnight. After washing, the membranes were incubated with horseradish peroxidase-linked secondary antibody, and bands were visualized using enhanced chemiluminescence system (GE Healthcare) and then exposing the blots. Protein levels were quantified by scanning densitometry (Alpha Image).
3
Immunofluorescence Staining of Cx43 and S100A4
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The unfixed frozen wound tissues segments were sectioned with a cryostat and placed on glass slides. Cut OCT-embedded tissues sections (10μm thick) were stained with analyzed immunofluorescence. Block each section with 2% BSA blocking buffer for 60 min at room temperature. Incubation for overnight at 4℃ with anti-Cx43 (1:1000, BD Transduction Labs); anti-S100A4 (1:500, Abcam). Sections were washed then second antibody incubated for 2 hrs at room temperature l with fluorescence-conjugated secondary antibodies Alexa Fluor® 488 goat anti-mouse (Invitrogen, Carlsbad, CA) and Alexa Fluor® 594 goat anti-rabbit (Invitrogen, Carlsbad, CA). Measurement of green and red fluorescence labeling by a laser scanning were imaged by confocal imaging system.
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