Anti mx1

Proteins were extracted from miR-204-, miR-211-, or miR-negative control-transfected MCF-7 cells using Pierce IP Lysis Buffer (Thermo Fisher Scientific, Waltham, MA, USA) with a protease inhibitor cocktail (Thermo Fisher Scientific). Diluted protein samples were separated on 4–12% gradient SDS-PAGE gels (Koma Biotech, Korea) and transferred to polyvinylidene difluoride membranes (Whatman, UK). Then, the membranes were blocked in 5% non-fat powdered milk (Bio Basic, Canada) for 1 h and incubated for 1 h with anti-MX1 (1:500, Santa Cruz, Dallas, Texas, USA) and anti-TXNIP (1:500, Novus, St. Charles, MO, USA) antibodies. The membranes were then rinsed in 0.01% Tween-20 TBS five times for 5 min each and incubated with horseradish peroxidase conjugated secondary donkey anti-rabbit antibody (1:5000, Amersham, UK) for 1 h. The bands were detected by an EzWay ECL Western Blot Substrate Kit (Koma Biotech) and Image Lab software (Bio-Rad, Hercules, CA, USA). As an endogenous reference, rabbit polyclonal anti-β-actin antibody (1:500) (Novus) was used.

The following antibodies were used for immunofluorescence or western blotting: Primary rabbit polyclonal: anti-actin (Sigma-Aldrich, A5060), anti-Daxx (Upstate, 07–471), anti-ATRX (Santa Cruz, H300), anti-PML (Bethyl Laboratories, A301-167A; Jena Biosciences, ABD-030), anti-Sp100 (GeneTex, GTX131569), anti-Mx1 (Santa Cruz, sc-50509; ProteinTech, 13750-1-AP), anti-ISG15 (ProteinTech, 15981-1-AP), anti-ISG54 (IFIT2, proteinTech, 12604-1-AP), and anti-histone H3 (abcam, ab1791). Primary mouse monoclonal: anti-HIRA (Millipore, 04–1488), anti-ICP0 (11060, [114 (link)]), anti-ICP4 (58s, [115 (link)]), anti-VP5 (DM165, [116 (link)]) anti-UL42 (Z1F11; [117 (link)]), and anti-PML (abcam, ab96051). Primary antibodies were detected using the following secondary antibodies: DyLight-680 or -800 conjugated goat anti-rabbit or -mouse (Thermo; 35568 and SA5-35571), Alexa -488, -555, or -647 conjugated donkey anti-rabbit or -mouse (Invitrogen; A21206, A21202, A31572, A31570, A31573, A31571), or HRP conjugated goat anti-mouse (Sigma-Aldrich, A4416).