Reaction courses and products mixtures where routinely monitored by TLC on silica gel Merck 60–200 mesh silica gel. Melting points were determined on a Stuart Scientific SMP3 apparatus and are uncorrected. 1H-NMR spectra were obtained in CDCl3, CD3OD solutions on a Varian Inova Unity (300 MHz) and Varian Direct Drive (400 MHz and 500 MHz). Chemical shifts (δ) are given in ppm upfield from tetramethylsilane. 13C-NMR spectra were obtained in CDCl3, CD3OD solutions on a Varian Direct Drive (125 MHz). All products reported showed 1H-NMR and 13C-NMR spectra in agreement with the assigned structures. Mass spectra were obtained by electrospray (ESY) with a LCT Premier XE Micromass Instrument (High resolution mass spectrometry).
Direct drive
Manufactured by Agilent Technologies
Sourced in United States
The Direct Drive is a laboratory equipment product from Agilent Technologies. It is designed to provide precise and reliable motion control for various applications in the laboratory setting. The core function of the Direct Drive is to enable accurate and reproducible movements for laboratory experiments and processes.
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Lab products found in correlation
15 protocols using direct drive
1
Characterization of Organic Compounds
2
NMR Resonance Assignment of Proteins
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Protein concentrations for resonance assignment were 0.5 mM for 15N/13C labeled and 0.8 mM for 15N labeled samples in 91 % H2O/9 % D2O (v/v) at pH 6.9, 10 mM sodium phosphate, 7 mM or 11.2 mM L-ascorbic acid, respectively. NMR experiments were carried out at 25 °C on a 600 MHz Bruker Avance II + spectrometer equipped with a Prodigy CryoProbe and on 500 MHz Agilent DirectDrive spectrometer equipped with a room-temperature probe. For resonance assignment we used 1H-15N-HSQC, 1H-13C-HSQC, and three-dimensional HNCO, HNCACB, CBCA(CO)NH, (H)CC(CO)NH-TOCSY, H(CC)(CO)NH-TOCSY, C,C-edited and C,N-edited methyl NOESY and 1H15N-HSQC-TOCSY experiments. Data were processed using NMRPipe (Delaglio et al. 1995 (link)) and analyzed with CcpNmr (Vranken et al. 2005 (link)).
3
Deuterium Metabolic MRI for Tumor Characterization
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The deuterium metabolic magnetic resonance experiments were designed as proof-of-principle, range-finding studies to discern the ability of the technique to discriminate pure tumor from pure RN. For these initial 2H experiments, we employed single-voxel spectroscopy (SPECIAL) performed at 11.74 T with an Agilent/Varian DirectDrive™ console and an Agilent/Magnex (Yarnton, Oxford, United Kingdom) horizontal superconducting magnet. 1H images, used for field shimming, planning, and anatomic registration of the 2H data, were collected with a 50-mm ID volume coil tuned to 499.3 MHz. 2H RF transmission and reception employ a one-turn 21-mm ID 2H surface coil tuned to 76.65 MHz. Anesthesia and physiologic monitoring were as described above for 1H MR imaging experiments.
4
Macaque Brain Diffusion MRI Protocols
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Macaque: we used ex vivo dMRI brain data of 6 rhesus macaques (4-16 years of age), publicly available from PRIME-DE (http://fcon_1000.projects.nitrc.org/indi/ PRIME/oxford2.html) (Milham et al. 2018) (link). Data were acquired using a 7T Agilent DirectDrive console, with a 2D diffusion-weighted spin-echo multi-shell (DW-SEMS) protocol (16 volumes acquired at b = 0 s/mm 2 , 128 volumes acquired at b = 4000 s/mm 2 ), at 0.6 mm isotropic resolution.
Human: Minimally preprocessed dMRI data (Glasser et al. 2013; (link)Sotiropoulos et al. 2013) (link) Iterative manual modification of ROIs within each template space was used, where needed, to obtain the final protocols. These protocols were used to produce population-averaged tract atlases in each template space
Human: Minimally preprocessed dMRI data (Glasser et al. 2013; (link)Sotiropoulos et al. 2013) (link) Iterative manual modification of ROIs within each template space was used, where needed, to obtain the final protocols. These protocols were used to produce population-averaged tract atlases in each template space
5
NMR Spectroscopy-Based Protein Structure Elucidation
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NMR experiments were carried out on 600 MHz Bruker Avance II+ and 500 MHz Agilent DirectDrive spectrometers at 25 °C. For backbone resonance assignment we used 1H-15N-HSQC, 1H-13C-HSQC, and HNCO, HN(CO)CA, HNCA, HNCACB, CBCA(CO)NH and 15N-HSQC-TOCSY (50 ms, 90 ms mixing time) triple resonance experiments. Data were processed using NMRPipe (Delaglio et al. 1995 (link)) and analyzed with CcpNmr (Vranken et al. 2005 (link)).
6
DNP-Enhanced Liquid-state Polarization
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The Liquid-state polarization measurements were acquired with 400 MHz Direct Drive console running VnmrJ 4.2 software and a 5 mm 1H/X broadband probe (Agilent, Palo Alto, CA, USA). The 13 C Larmor frequency at the three field strengths are 35.9, 71.8 and 108 MHz, respectively. A flip angle of 5° every 5 s was used to measure the decay of the DNP enhanced NMR signal. T1 and initial polarization (at time of dissolution) was calculated from the exponential decay. Liquid state polarization was measured by normalization of the DNP enhanced signal with the NMR signal at thermal equilibrium for the same sample using a 90° flip angle.
7
Multimodal Brain Imaging at 4T
8
MRI Characterization of Brain Tissue Microstructure
9
In Vivo Neuroimaging of WT and SR−/− Mice
10
Dynamic MRI Tracking of SPIO Nanoparticles
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The mice were examined in a 9.4 T/31 cm horizontal bore magnet with a 21 cm ID gauss/cm and a 12 cm ID gauss/cm gradient tube and interfaced to a Varian Direct Drive console (Varian, Palo Alto, CA, USA). A 35 mm ID volume coil was used to all MR images. During imaging, the free-breathing mice were sedated by 1–1.5% isoflurane mixed with oxygen, and the core temperature, electrocardiogram (ECG) and respiration were monitored (SA Inc, Stony Brook, NY). The core temperature was maintained at 37°C by directing warm air into the bore, while the respiration signals were used to gate the data acquisition to minimize motion interference.
Dynamic T2*-weighted MR imaging was performed using a two-dimensional T2-weighted gradient-echo sequence (TR/TE = 7/3.5 ms, flip angle 10°, matrix of 128×128, a 30×30 mm field of view). Transversal images of the abdomen containing the liver, the stomach, and major abdominal vessels (aorta, inferior vena cava and portal vein) were consecutively taken every 3.2 s. This sequence was applied continuously 440 measurements. Injection of SPIO nanoparticles was started at measurement 34, directly followed by a 100 µl saline flush. The SPIO nanoparticle was administered manually in approximately 25 s. In consequence, the kinetics of distribution of SPIO nanoparticles can then be imaged in vivo by measuring dynamic time series.
Dynamic T2*-weighted MR imaging was performed using a two-dimensional T2-weighted gradient-echo sequence (TR/TE = 7/3.5 ms, flip angle 10°, matrix of 128×128, a 30×30 mm field of view). Transversal images of the abdomen containing the liver, the stomach, and major abdominal vessels (aorta, inferior vena cava and portal vein) were consecutively taken every 3.2 s. This sequence was applied continuously 440 measurements. Injection of SPIO nanoparticles was started at measurement 34, directly followed by a 100 µl saline flush. The SPIO nanoparticle was administered manually in approximately 25 s. In consequence, the kinetics of distribution of SPIO nanoparticles can then be imaged in vivo by measuring dynamic time series.
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