Double distilled water was used in all experiments. Molecular biology grade chemicals and reagents were purchased from Sigma-Aldrich or Fisher Scientific. Ni2+-NTA chromatography resin and thrombin cleavage capture kits were purchased from EMD-Millipore. Superdex 10/300 GL columns for AKTA FPLC were purchased from GE Healthcare Life Sciences. Protein assay kits and protein markers for SDS-PAGE were purchased from Bio-Rad Laboratories. Amicon spin concentrators were purchased from EMD-Millipore. Plasmid MiniPrep Kits were purchased from Qiagen, and Biacore-Sensor Chip NTA and buffers used in Surface Plasmon Resonance (SPR) analyses were purchased from GE Healthcare Life Sciences.
Ni2 nta chromatography resin
Manufactured by Merck Group
Ni2+-NTA chromatography resin is a solid-phase affinity chromatography material used for the purification of proteins with a histidine tag. The resin consists of nickel-nitrilotriacetic acid (Ni2+-NTA) groups covalently bound to an agarose or polymer support matrix. The Ni2+ ions coordinate with the histidine residues on the target protein, allowing it to be selectively bound and separated from other components in the sample.
Automatically generated - may contain errors
Lab products found in correlation
Protein assay kit, by Bio-Rad (2 mentions)
Plasmid miniprep kit, by Qiagen (2 mentions)
Molecular biology grade reagents and chemicals, by Merck Group (2 mentions)
Thrombin cleavage capture kit, by Merck Group (2 mentions)
Amicon spin concentrator, by Merck Group (2 mentions)
Superdex 10 300 gl columns for akta fplc, by GE Healthcare (2 mentions)
Protein markers for sds page, by Bio-Rad (2 mentions)
Biacore sensor chip nta, by GE Healthcare (2 mentions)
2 protocols using ni2 nta chromatography resin
1
Protein Purification and Characterization
2
Protein Purification Using Ni2+ Chromatography
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Double distilled water was used in all experiments. Molecular biology grade chemicals and reagents were purchased from Sigma-Aldrich or Fisher Scientific. Ni2+-NTA chromatography resin and thrombin cleavage capture kits were purchased from EMD-Millipore. Superdex 10/300 GL columns for AKTA FPLC were purchased from GE Healthcare Life Sciences. Protein assay kits and protein markers for SDS-PAGE were purchased from Bio-Rad Laboratories. Amicon spin concentrators were purchased from EMD-Millipore. Plasmid MiniPrep Kits were purchased from Qiagen, and Biacore-Sensor Chip NTA and buffers used in surface plasmon resonance (SPR) analyses were purchased from GE Healthcare Life Sciences.
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