TSE1 (Figure 7 ) was purchased from Yingshili Biotechnology (Hangzhou, Zhejiang, China), and was dissolved in dimethyl sulfoxide (DMSO) at 100 mM and stored at −20 °C PLT, 6-well plates, 24-well plates, 96-well plates, Transwell chambers and Corning Matrigel Basement Membrane Matrix High Concentration were from Corning (Corning, NY, USA). Aqueous One Solution Cell Proliferation assay (MTS) was purchased from Promega Corporation (Madison, WI, USA). RPMI-1640 medium was purchased from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS) was from Invitrogen (Grand Island, NY, USA). ALDEFLUOR Stem Cell Identification Kit, MammoCult Medium Human Kit, Hydrocortisone Stock Solution, 0.2% Heparin Solution and HBSS were obtained from Stem Cell Technologies (Vancouver, BC, Canada). Primary antibodies for Oct-4, CD44, Nanog, Bcl-2, Notch-1, MMP-9 and horseradish peroxidase-conjugated secondary antibody were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). Antibody for GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA).
Aqueous one solution cell proliferation assay mts
Manufactured by Promega
Sourced in United States
The Aqueous One Solution Cell Proliferation assay (MTS) is a colorimetric method for determining the number of viable cells in proliferation or cytotoxicity assays. The assay employs a tetrazolium compound (MTS) that is bioreduced by cells into a colored formazan product that is soluble in tissue culture medium. The quantity of formazan product is directly proportional to the number of living cells in culture.
Automatically generated - may contain errors
Lab products found in correlation
Horseradish peroxidase conjugated secondary antibody, by Cell Signaling Technology (2 mentions)
Mammocult human medium kit, by STEMCELL (2 mentions)
96 well plate, by Corning (2 mentions)
Transwell chamber, by Corning (2 mentions)
24 well plate, by Corning (2 mentions)
6 well plate, by Corning (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Matrigel basement membrane matrix high concentration, by Corning (2 mentions)
Notch1, by Cell Signaling Technology (2 mentions)
Nanog, by Cell Signaling Technology (2 mentions)
Heparin solution, by STEMCELL (2 mentions)
Hydrocortisone stock solution, by STEMCELL (2 mentions)
Bcl 2, by Cell Signaling Technology (2 mentions)
Rpmi 1640 medium, by Merck Group (2 mentions)
Antibody for gapdh, by Santa Cruz Biotechnology (2 mentions)
Hanks balanced salt solution (hbss), by STEMCELL (2 mentions)
Aldefluor stem cell identification kit, by STEMCELL (2 mentions)
Mmp 9, by Cell Signaling Technology (2 mentions)
Skov3 cell line, by American Type Culture Collection (1 mentions)
Effectene transfection reagent, by Qiagen (1 mentions)
3 protocols using aqueous one solution cell proliferation assay mts
1
Establishing Cancer Stem Cell Assays
2
Stem Cell Characterization Protocol
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TSE1 (Figure 7 ) was purchased from Yingshili Biotechnology (Hangzhou, Zhejiang, China), and was dissolved in dimethyl sulfoxide (DMSO) at 100 mM and stored at −20 °C PLT, 6-well plates, 24-well plates, 96-well plates, Transwell chambers and Corning Matrigel Basement Membrane Matrix High Concentration were from Corning (Corning, NY, USA). Aqueous One Solution Cell Proliferation assay (MTS) was purchased from Promega Corporation (Madison, WI, USA). RPMI-1640 medium was purchased from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS) was from Invitrogen (Grand Island, NY, USA). ALDEFLUOR Stem Cell Identification Kit, MammoCult Medium Human Kit, Hydrocortisone Stock Solution, 0.2% Heparin Solution and HBSS were obtained from Stem Cell Technologies (Vancouver, BC, Canada). Primary antibodies for Oct-4, CD44, Nanog, Bcl-2, Notch-1, MMP-9 and horseradish peroxidase-conjugated secondary antibody were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). Antibody for GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA).
3
Lipid Nanoparticle Formulation and Characterization
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1,2-Dioleoyl-3-trimethylammonium-propane (DOTAP) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethyleneglycol)-2000] (DSPE-PEG-2000) were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL). Chol, chloroform, McCoy’s 5a modified growth media, and fetal bovine serum were purchased from Millipore Sigma (St. Louis, MO). The Aqueous One Solution Cell Proliferation Assay (MTS) was obtained through Promega (Madison, WI). The 5 × DNA loading buffers were purchased from Thomas Scientific (Swedesboro, NJ). The single-stranded fluorescent-labeled oligonucleotide 5′–[6-FAM] CTCCTCCCATTTTTATAAG–3′ (27 ) was obtained from Eurofins MWG Operon (Huntsville, AL). SK-OV-3 cell line was purchased from the American Type Culture Collection (Manassas, VA). JetPRIME® (JP) reagent for mRNA transfection was purchased from Polyplus Transfection (New York, NY). Effectene transfection reagent (obtained from Qiagen, Germantown, MD) and plasmid DNA were kindly gifted to us by Dr. Mohammad Ali (Binghamton University).
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