Tissue samples were fixed in 10% formalin for 48 hr, dehydrated, and embedded in paraffin. Sections (8 μm) were cut and stained with hematoxylin and eosin (American Master Tech Scientific). Fibrosis was assessed by picrosirius red staining (Sigma), and the positive area for fibrosis was quantified with Image J software (Schneider et al., 2012 (link)). For wheat germ agglutinin (WGA) immunofluorescence, 8 μm heart sections were prepared, washed in PBS, incubated for 2 hr in WGA-Alexa 488 lectin (Invitrogen, Carlsbad, CA, USA), and washed and mounted in anti-fade reagent. Four images (×20) were taken from each heart, and the areas of 100–200 cross-sectionally oriented cardiomyocytes were measured and analyzed with Image J software (Schneider et al., 2012 (link)).
Wga alexa 488 lectin
Manufactured by Thermo Fisher Scientific
Sourced in United States
The WGA-Alexa 488 lectin is a fluorescently labeled lectin used for the detection and visualization of glycoconjugates in biological samples. It binds to N-acetylglucosamine and sialic acid residues, which are commonly found on the surface of cells and in the extracellular matrix. The Alexa Fluor 488 dye provides bright green fluorescence that can be detected using standard fluorescence microscopy or flow cytometry techniques.
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Lab products found in correlation
3 protocols using wga alexa 488 lectin
1
Quantitative Analysis of Cardiac Fibrosis and Cardiomyocyte Size
2
Histological Analysis of Cardiac Fibrosis
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Tissue samples were fixed in 10% formalin for 48 h, dehydrated and embedded in paraffin. Sections (8 μm) were cut and stained with haematoxylin and eosin (American Master Tech Scientific). Fibrosis was assessed with Picrosirius red staining (Sigma). For wheatgerm agglutinin (WGA) immunofluorescence, 8 μm heart sections were prepared, washed in PBS, incubated for 2 h in WGA-Alexa 488 lectin (Invitrogen, Carlsbad, CA, USA), and washed and mounted in anti-fade reagent. Four images ( × 20) were taken from each heart, and the diameter and areas of 100–200 cross-sectionally oriented myocytes were measured and analysed with Image J software.
3
Quantifying Cardiac Fibrosis and Cardiomyocyte Size
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Master Tech Scientific). Fibrosis was assessed by Picrosirius red staining (Sigma) and the positive area for fibrosis was quantified with Image J software (Schneider et al, 2012) heart sections were prepared, washed in PBS, incubated for 2h in WGA-Alexa 488 lectin (Invitrogen, Carlsbad, CA, USA), and washed and mounted in anti-fade reagent. Four images (×20)
were taken from each heart, and the areas of 100 200 cross-sectionally oriented cardiomyocytes were measured and analyzed with Image J software (Schneider et al., 2012) .
were taken from each heart, and the areas of 100 200 cross-sectionally oriented cardiomyocytes were measured and analyzed with Image J software (Schneider et al., 2012) .
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