The following AHL standards, purchased from University of Nottingham, UK were: N-3-oxo-butyryl-L-homoserine lactone (3OC4-HSL), N-3-hydroxy-butyryl-L-homoserine lactone (3OHC4-HSL), N-3-hydroxy-hexanoyl-L-homoserine lactone (3OHC6-HSL), N-3-hydroxy-octanoyl-L-homoserine lactone (3OHC8-HSL), N-3-hydroxy-decanoyl-L-homoserine lactone (3OHC10-HSL). In addition, N-butyryl-DL-homoserine lactone (C4-HSL), N-hexanoyl-L-homoserine lactone (C6-HSL), N-3-oxo-hexanoyl-L-homoserine lactone (3OC6-HSL), N-octanoyl-L-homoserine lactone (C8-HSL), N-3-oxo-octanoyl-L-homoserine lactone (3OC8-HSL), N-decanoyl-DL-homoserine lactone (C10-HSL), N-3-oxo-decanoyl-L-homoserine lactone (3OC10-HSL), N-dodecanoyl-DL-homoserine lactone (C12-HSL), N-3-oxo-dodecanoyl-L-homoserine lactone (3OC12-HSL), N-3-hydroxy-dodecanoyl-DL-homoserine lactone (3OHC12-HSL), acetonitrile, and formic acid for HPLC were obtained from Sigma-Aldrich (St. Louis, MO, USA).
N 3 oxo decanoyl l homoserine lactone
Manufactured by Merck Group
Sourced in United States
N-3-oxo-decanoyl-L-homoserine lactone is a chemical compound used in laboratory research. It functions as a signaling molecule involved in quorum sensing, a process of cell-to-cell communication in bacteria. The compound is commonly used in studies related to microbial behavior and intercellular coordination.
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Lab products found in correlation
N 3 oxo octanoyl l homoserine lactone, by Merck Group (4 mentions)
N 3 oxo dodecanoyl l homoserine lactone, by Merck Group (3 mentions)
N butyryl dl homoserine lactone, by Merck Group (3 mentions)
N hexanoyl l homoserine lactone, by Merck Group (3 mentions)
N decanoyl dl homoserine lactone, by Merck Group (2 mentions)
N dodecanoyl dl homoserine lactone, by Merck Group (2 mentions)
N 3 oxo hexanoyl l homoserine lactone, by Merck Group (2 mentions)
N octanoyl l homoserine lactone, by Merck Group (2 mentions)
N 3 hydroxy dodecanoyl dl homoserine lactone, by Merck Group (2 mentions)
Formic acid for hplc, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
N acylhomoserine lactones, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Hplc grade acetonitrile, by Merck Group (1 mentions)
Paraoxon, by Merck Group (1 mentions)
P nitrophenol, by Merck Group (1 mentions)
Methyl parathion, by Merck Group (1 mentions)
Diazinon, by Merck Group (1 mentions)
Malathion, by Merck Group (1 mentions)
Dimethoate, by Merck Group (1 mentions)
4 protocols using n 3 oxo decanoyl l homoserine lactone
1
AHL Compound Standards for Quorum Sensing
2
Marine Bacteria Quorum Sensing Inhibition
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The marine bacterial strains analysed in this study are summarized in Table 2 . Marine agar or broth (MA or MB respectively) was routinely used to grow marine bacterial strains (Difco, Oxford, UK). SYP agar with 1.5% of sea salt and MA or MB were used in different experiments performed in this work to analyse the potential QSI capability of marine bacteria. All marine bacterial strains used in this study were grown routinely at 23°C. Different N‐Acyl homoserine lactones (AHLs) used in this work were purchased from Sigma‐Aldrich (Cambridge, UK) and were dissolved in DMSO (N‐butyryl‐dl ‐homoserine lactone: C4‐HSL; N‐hexanoyl‐l ‐homoserine lactone: C6‐HSL; N‐(3‐oxooctanoyl)‐l ‐homoserine lactone: 3OC8‐HSL; N‐(3‐oxodecanoyl)‐l ‐homoserine lactone: 3OC10‐HSL; and N‐(3‐oxododecanoyl)‐l ‐homoserine lactone: 3OC12‐HSL).
3
Analytical Standards for AHL Quorum Sensing
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The following AHL standards were purchased from University of Nottingham, UK: N-3-oxo-butyryl-L-homoserine lactone (3-oxo-C4-HSL), N-3-hydroxy-butyryl-L-homoserine lactone (3-OH-C4-HSL), N-3-hydroxy-hexanoyl-L-homoserine lactone (3-OH-C6-HSL), N-3-hydroxy-octanoyl-L-homoserine lactone (3-OH-C8-HSL), N-3-hydroxy-decanoyl-L-homoserine lactone (3-OH-C10-HSL). Standards purchased from Sigma-Aldrich were: N-butyryl-DL-homoserine lactone (C4-HSL), N-hexanoyl-L-homoserine lactone (C6-HSL), N-3-oxo-hexanoyl-L- homoserine lactone (3-oxo-C6-HSL), N-octanoyl-L-homoserine lactone (C8-HSL), N-3-oxo-octanoyl-L-homoserine lactone (3-oxo-C8-HSL), N-decanoyl-DL-homoserine lactone (C10-HSL), N-3-oxo-decanoyl-L-homoserine lactone (3-oxo-C10-HSL), N-dodecanoyl-DL-homoserine lactone (C12-HSL), N-3-oxo-dodecanoyl-L-homoserine lactone (3-oxo-C12-HSL), and N-3-hydroxy-dodecanoyl-DL-homoserine lactone (3-OH-C12-HSL). HPLC grade acetonitrile and formic acid were purchased from Merck.
4
Pesticide and Quorum Sensing Compound Acquisition
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Parathion, methyl parathion, diazinon, chlorpyrifos, malathion, dimethoate, and demeton-S-methyl were purchased from Supelco (Bellefonte, PA, USA). Poly(vinyl alcohol) 16/1 (84 kDa) was purchased from Sinopec Corp. (Beijing, China). Paraoxon, p-nitrophenol, N-homoserine lactone hydrochloride, N-butyryl-D,L-homoserine lactone, N-(3-oxooctanoyl)-L-homoserine lactone, N-(3-oxodecanoyl)-L-homoserine lactone, N-(3-oxododecanoyl)-L-homoserine, and other chemicals were acquired from Sigma-Aldrich (St. Louis, MO, USA).
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