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Potassium chloride (kcl)

Sourced in China

Potassium Chloride (KCl) is a chemical compound that is commonly used in various laboratory applications. It is a white, crystalline solid with a chemical formula of KCl. KCl is a salt that dissociates into potassium (K+) and chloride (Cl-) ions in aqueous solutions.

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24 protocols using potassium chloride (kcl)

1

Synthesis of Polymerized Anionic Drilling Fluid

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AM (99 wt%), DMC (75 wt% solutions), acetonitrile (99 wt%), diallyl dimethylammonium chloride (60 wt% solutions), potassium persulfate (99.5 wt%), sodium bisulfite (99.9 wt%), polyether amine (average Mn~230), and potassium chloride (99.5 wt%) were purchased from Shanghai Mclean Biochemical Technology Co., Ltd. (Shanghai, China) Bentonite was purchased from Huai’an County Tengfei bentonite Development Co., Ltd. (Huai’an, China) Polyanionic cellulose (PAC-LV) was purchased from Shandong Deshunyuan Petroleum Technology Co., Ltd. (Dongying, China) Shale cuttings and outcrops are from Sichuan, China. VAPIMBr was produced using the substitution reaction of 1-vinylimidazole and 3-bromopropylamine hydrobromide in acetonitrile.
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2

Bioactive Compound Extraction from Pleurotus eryngii

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Main materials: XG gum of a food grade was provided by Usolf Co., Ltd. (Linyi, China); potato flour and potato starch were bought from Modern Agriculture Service Co., Ltd. (Zhengyang, China); wheat bran was provided by Qianjiang Tongguang Flour Co., Ltd. (Qianjiang, China); sodium chloride, lipase, pepsin, amylase and amyloglucosidase were bought from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China); 3,5-dinitrosalicylic acid, beta Dextran standard, glucose standard, and Congo red dye were provided by McLean Biochemical Technology Co., Ltd. (Shanghai, China).
Main solvents: glycerol; phosphate buffer; sodium acetate buffer; potassium chloride buffer; Pleurotus eryngii liquid fermentation broth: the triangular flask was filled with liquid medium, the activated slant strains were inserted, and the culture was shaken at 28 °C and at 180 r/min for 8 days.
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3

A. fumigatus cultivation and materials

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A. fumigatus (A1161) was obtained from Professor Ling Lu’s lab (Jiang et al., 2014 (link)). AgNO3 (99.8%) and domiphen (97%) were purchased from Sinopharm Chemical Reagent Co., Ltd. (China) and Shanghai Yien Chemical Technology Co., Ltd. (China), respectively. Artemisia argyi leaf extract and glucose were purchased from Xi’an Runxue Bio-technology Co., Ltd. (China) and Aladdin Reagent Co., Ltd. (China), respectively. Agar and RPMI-1640 were purchased from Xuzhou Microcomande Biological Engineering Co., Ltd. (China) and Shanghai Sigma-Aldrich Trading Co., Ltd. (China), respectively. Yeast extract, ZnSO4·7H2O, H3BO3, MnCl2·4H2O, FeSO4·7H2O, CoCl2·5H2O, CuSO4·5H2O, (NH4)6Mo7O24·4H2O, Na4EDTA, NaNO3, KCl, CuCl2 ammonium tartrate (C4H12N2O6), MgSO4·7H2O and KH2PO4 were purchased from Shanghai Maclin Biochemical Technology Co., Ltd. (China). For RNA-seq, A. fumigatus strains were grown on liquid MM (minimal medium), containing 1% glucose, trace elements and 50 ml L−1 20 × salt, pH 6.5. For the plate inoculation tests, A. fumigatus strains were grown on rich medium-YAG (yeast extract, agar and glucose), containing 2% glucose, trace elements, 0.5% yeast extract and 2% agar (Du et al., 2021b (link)).
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4

Thionine-based electrochemical biosensor development

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Thionine (Thi) was obtained
from Alfa Aesar Chemical (Shanghai, China). Reagents including N-(3-(dimethylamino)propyl)-N′-ethylcarbodiimide
hydrochloride (EDC), cysteamine hydrochloride (C2H7NS-HCl), Tween 20, ethanolamine (C2H7NO), potassium ferricyanide (K3[Fe(CN)6]),
potassium ferrocyanide (K4[Fe(CN)6]), and bovine
serum albumin (BSA) were sourced from Aladdin Bio-Chem Technology
Co., Ltd. (Shanghai, China). Additional chemicals such as N-hydroxysulfosuccinimide sodium salt (NHS), glutaraldehyde
(C5H8O2), MES buffer, and KCl were
produced from McLean Biochemical Technology Co., Ltd. (Shanghai, China).
A phosphate-buffered saline (PBS) solution (pH 7.4) was provided by
Sangon Biotech (Shanghai) Co., Ltd. (Shanghai, China). Capture antibody
(Ab1) and detection antibody (Ab2) were prepared
by HyTest Biotech Co., Ltd. (Turku, Finland). TNF-alpha antigen was
acquired from MedChemExpress Biotechnology Co., Ltd. (New Jersey,
United States).
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5

In Vitro Digestion and Antioxidant Assays

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Sodium chloride (NaCl, 99%), potassium chloride (KCl, >99%), sodium hydrogen carbonate (NaHCO3, >99%), calcium chloride (CaCl2, >98%), hydrochloric acid (HCl, 37%), ammonium chloride (NH4Cl), sodium dihydrogen phosphate monohydrate (NaH2PO4·H2O, >99%), potassium dihydrogen phosphate (KH2PO4), magnesium chloride (MgCl2, >99%), urea (≥99%), potassium persulfate (K2S2O8, >99%), potassium ferricyanide, and trichloroacetic acid (TCA) were obtained from Shanghai Macklin Biochemical Co. Ltd. (Shanghai, China). Folin–Ciocalteu reagent, pepsin (from porcine gastric mucosa), mucin (from the porcine stomach), pancreatin (from the porcine pancreas), lipase (from the porcine pancreas), bile extract porcine, 2-diphenyl-1-picrylhydrazyl (DPPH, 95%), and 2,2′-azinobis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS, >98%) were obtained from Sigma-Aldrich.
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6

Cytotoxicity Assay Protocol

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All chemicals were commercially available, of analytical grade, and used without further purification. CA, urea, ethanol, formic acid, ethyl acetate, sodium chloride, potassium chloride, sodium dihydrogen phosphate, and monopotassium phosphate were purchased from the Macklin Biochemical Co., Ltd. (Shanghai, China). Milli-Q water (resistivity 18.8 MΩ) was used in all experiments. Roswell Park Memorial Institute-1640 medium (PRMI-1640) was purchased from GIBCO (Thermo, USA) and fetal bovine serum (FBS) was purchased from ExCell Bio (Shanghai, China). Cell Counting Kit-8 (CCK-8) solution was purchased from Yeasen (Shanghai, China).
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7

Synthesis of Mg Alloy AZ31B

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AZ31B Mg alloy (composition: 2.5–3.5 Al, 0.6–1.4 Zn, 0.2–1.0 Mn, 0.04 Ca, 0.08 Si, 0.003 Fe, 0.001 Ni, 0.01 Cu, Mg, all in wt. %) was purchased from Luoyang Shengte Metalware Co., Ltd. (Luoyang, China). 2,5-dihydroxyterephthalic acid (98%), sodium hydrogen carbonate (99.8%), potassium chloride (99.5%), di-potassium hydrogen phosphate trihydrate (99%), magnesium chloride hexahydrate (98%), calcium chloride (96%), sodium sulfate (99%), and tris-hydroxymethyl aminomethane (AR) were bought from the Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China). Magnesium nitrate hexahydrate (99%) and anhydrous methanol were bought from the Tianjin Hengxing Chemical Reagent co., Ltd. (Tianjin, China). Ethanol and sodium chloride (99.5%) were bought from the Tianjin Chemical Reagent Supply and Marketing Company (Tianjin, China). Hydrofluoric acid (40%) was bought from the Luoyang Haohua Chemical Reagent Co., Ltd. (Luoyang, China). N,N-dimethylformamide (99.5%) was bought from the Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Hydrochloric acid (36–38%) was purchased from the Zhengzhou Paini Chemical Reagent Factory (Zhengzhou, China).
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8

Synthesis of Inorganic Compounds

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Ferric chloride hexahydrate (FeCl3·6H2O), cadmium chloride (CdCl2·5/2H2O), potassium chloride (KCl), ferrosoferric oxide (Fe3O4), potassium ferricyanide (K3Fe(CN)6), and hydrochloric acid (HCl) were purchased from Macklin and Beijing Chemical Works, China.
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9

Electrochemical Sensing of Neurotransmitters and Antioxidants

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Potassium chloride (KCl), potassium ferrocyanide trihydrate (K4Fe(CN)6·3H2O), iron(iii) chloride (FeCl3), dopamine hydrochloride (DA), l-ascoribic acid (AA), uric acid (UA), and phosphate buffered saline buffer (PBS buffer, pH 7.4) premixed powder were obtained from Macklin, and sulfuric acid (H2SO4) was purchased from Sinopharm Chemical Reagent Co., Ltd. All chemicals were used as received. Ultrapure water (18.2 MΩ cm) with total organic carbon (TOC) ≪ 3 ppb was obtained from a Milli-Q 3 UV system (Millipore) and used for all aqueous solutions. The quartz capillaries (Q100-50-7.5, o.d. = 1 mm, i.d. = 0.5 mm and length = 7.5 cm) were from Sutter Instrument Co.
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10

Characterization of Corn Starches

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Waxy corn starch (WCS) and normal corn starch (NCS) were purchased from Dezhou Dacheng Food Co., Ltd. (Dezhou, China). Two kinds of high-amylose corn starch, labeled G50 and G70, were supplied by Gansu Kunlun Biochemical Co., Ltd. (Zhangye, China) and Hainan Shanliang Technology Co., Ltd. (Haikou, China), respectively. The information on amylose content is listed in Table 1.
All chemical reagents, including anhydrous calcium chloride, sodium bromide, and potassium chloride, were purchased from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, Country) and were analytically pure. Lithium bromide, silicone oil (DC 200), and an amylose standard were purchased from Sigma-Aldrich (Shanghai, China) Trading Co., Ltd. (Shanghai, Country); dimethyl sulfoxide was purchased from ANPEL Laboratory Technologies Inc. (Shanghai, China).
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