Vlx 600

Manufactured by Cayman Chemical

Sourced in United States

The VLX-600 is a laboratory centrifuge capable of processing samples up to 600 milliliters in volume. It is designed for general-purpose applications in research and clinical settings.

Automatically generated - may contain errors

Lab products found in correlation

Lipopolysaccharide lps from e coli, by Merck Group (1 mentions) Trimetazidine tmz, by Merck Group (1 mentions) Rpmi 1640 with glutamax, by Thermo Fisher Scientific (1 mentions) Cb 839, by Focus Biomolecules (1 mentions) Interleukin 4 (il 4), by BioLegend (1 mentions) M csf, by BioLegend (1 mentions) Penicillin streptomycin p s, by Thermo Fisher Scientific (1 mentions) Ifn γ, by BioLegend (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Wzb117, by Selleck Chemicals (1 mentions) Tigecycline, by Selleck Chemicals (1 mentions) Mycoalert mycoplasma detection kit, by Lonza (1 mentions) Rpmi 1640 medium, by Corning (1 mentions)

2 protocols using vlx 600

Reagents for Macrophage Metabolism Analysis

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RPMI-1640 with Glutamax, non-essential amino acids (NEAA), sodium pyruvate, and penicillin–streptomycin (P/S) were from Gibco (Paisley, Scotland, UK). Fetal bovine serum (FBS) was purchased from Life Science Production (Barnet, UK). Cytokines M-CSF, IL-4, and IFN-γ were purchased from Biolegend (San Diego, CA, USA). 2-Deoxy-D-glucose (2-DG), Oligomycin (Oligo), Carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone (FCCP), Rotenone (R/Rot,), and Antimycin A (AA) were purchased from Abcam (Cambridge, UK). Perhexiline (PerHx) and H531 (Hx) were purchased from Tocris (Bristol, UK). CB-839 was purchased from FOCUS Biomolecules (Plymouth Meeting, PA, USA). Trimetazidine (TMZ) was purchased from Sigma-Aldrich (St. Louis, MO, USA). VLX-600 (VLX) was purchased from Cayman Chemical (Ann Arbor, MI, USA). Lipopolysaccharide (LPS) from E. Coli was obtained from Sigma-Aldrich. Any other material used was of analytical grade.

Oyarce C., Vizcaino-Castro A., Chen S., Boerma A, & Daemen T. (2021). Re-polarization of immunosuppressive macrophages to tumor-cytotoxic macrophages by repurposed metabolic drugs. Oncoimmunology, 10(1), 1898753.

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Ovarian Cancer Cell Line Characterization

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OVCAR-8 and OVCAR-5 cells were kind gifts from D. Scudierio (NCI, National Institutes of Health). The PEA1 cell line was from Sigma-Aldrich. The BRCA1-mutant COV362 cell line was a gift from Robert van Waardenburg, University of Alabama Birmingham. The cells were cultured in RPMI-1640 medium (Corning) supplemented with 8% fetal bovine serum (Millipore), and maintained in a humidified 37°C, 5% CO₂ incubator. All cells were authenticated by autosomal STR profiling (University of Arizona Genetics Core). All cell lines were free of Mycoplasma contamination as determined testing with a MycoAlert Mycoplasma Detection Kit (Lonza). VLX600 was obtained from Cayman Chemical. Tigecycline and WZB117 were obtained from Selleck Chemicals.

Kanakkanthara A., Kurmi K., Ekstrom T.L., Hou X., Purfeerst E.R., Heinzen E.P., Correia C., Huntoon C.J., O’Brien D., Hendrickson A.E., Dowdy S.C., Li H., Oberg A.L., Hitosugi T., Kaufmann S.H., Weroha S.J, & Karnitz L.M. (2019). BRCA1 Deficiency Upregulates NNMT Which Reprograms Metabolism and Sensitizes Ovarian Cancer Cells to Mitochondrial Metabolic Targeting Agents. Cancer research, 79(23), 5920-5929.

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