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Lentivirus plasmids plko 1 shegfr

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The Lentivirus plasmids (pLKO.1-shEGFR) are molecular biology tools designed for gene knockdown experiments. They contain a short hairpin RNA (shRNA) sequence targeting the EGFR gene, which can be used to induce RNA interference and suppress EGFR expression in target cells. The plasmid backbone includes elements necessary for lentiviral packaging and transduction.

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Lab products found in correlation

P erk1 2 thr202 tyr204, by Cell Signaling Technology (1 mentions) Butein, by Merck Group (1 mentions) P akt ser473, by Cell Signaling Technology (1 mentions) P egfr tyr1068, by Cell Signaling Technology (1 mentions) β actin antibody a5316, by Merck Group (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Cleaved parp, by Cell Signaling Technology (1 mentions) Bcl xl, by Cell Signaling Technology (1 mentions) Mcl 1, by Cell Signaling Technology (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Anti rabbit igg hrp, by Cell Signaling Technology (1 mentions) Anti mouse igg hrp, by Cell Signaling Technology (1 mentions) Hep3b, by American Type Culture Collection (1 mentions) Anti c fos, by Cell Signaling Technology (1 mentions) Anti p erk1 2 thr202 tyr204, by Cell Signaling Technology (1 mentions) Anti p egfr tyr1068, by Cell Signaling Technology (1 mentions) Isoliquiritigenin, by Selleck Chemicals (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Anti cyclin d1, by Cell Signaling Technology (1 mentions) Anti fosb, by Cell Signaling Technology (1 mentions)

2 protocols using lentivirus plasmids plko 1 shegfr

1

Butein Induces Apoptosis in Cancer Cells

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Hep3B and the human embryonic kidney cell 293T were obtained from American Type Culture Collection (Manassas, VA, USA). Huh-7 cells were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Hep3B, 293T, and Huh7 cells were cultured with Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% antibiotics. Butein, β-actin (A5316) antibody, and the chemical reagents used in this study were products of Sigma-Aldrich (St. Louis, MO, USA). The antibodies of HK2 (#2867), VDAC1(#4866), BCL2 (#2870), BCL-XL (#2764), MCL-1 (#5453), cleaved PARP (#5625), cleaved caspase-3 (#9664), EGFR (#4267), p-EGFR-Tyr1068 (#3777), p-Akt-Ser473 (#4060), p-ERK1/2-Thr202/Tyr204 (#8544), anti-rabbit IgG HRP (#7074), and anti-mouse IgG HRP (#7076) were obtained from Cell Signaling Technology (Beverly, MA, USA). Lentivirus plasmids (pLKO.1-shEGFR) were products of Thermo Scientific (Huntsville, AL, USA). Lipofectamine™ 2000 was obtained from Invitrogen (Carlsbad CA, USA).
Liao W., Liu J., Zhang D., Huang W, & Chen R. (2018). Butein Inhibited In Vitro Hexokinase-2-Mediated Tumor Glycolysis in Hepatocellular Carcinoma by Blocking Epidermal Growth Factor Receptor (EGFR). Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 24, 3283-3292.
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2

ESCC Cell Line Characterization and Compound Screening

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The esophageal squamous cell carcinoma (ESCC) cells KYSE140, KYSE520, and TE1 were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). All cells were subjected to mycoplasma examination and cytogenetically test and cultured with completed culture medium (basic medium supplemented with 10% fetal bovine serum) according to the standard protocols. The compound isoliquiritigenin was purchased from Selleck Chemicals (Houston, TX). Recombinant human EGF was product of R&D. The primary antibodies used in this study including anti-p-EGFR (Tyr1068) (#3777), anti-EGFR (#4267), anti-p-Akt (S473) (#4060), anti-Akt (#4691), anti-p-ERK1/2 (Thr202/Tyr204) (#4370), anti-ERK1/2(#4695), anti-c-Jun (#9165), anti-JunB (#3746), anti-JunD (#5000), anti-FosB(#4691), anti-c-Fos (#2251), anti-Fra1(#5281), anti-Cyclin D1(#55506), anti-β-actin (#3700), and anti-Histone H3 Ser10 (#53348) were products of Cell Signaling Technology (Danvers, MA). Lentivirus plasmids (pLKO.1-shEGFR) were purchased from Thermo Scientific (Huntsville, AL, USA).
Ye L., Zhang J., Zhang Y., Gu B., Zhu H, & Mao X. (2020). Isoliquiritigenin Suppressed Esophageal Squamous Carcinoma Growth by Blocking EGFR Activation and Inducing Cell Cycle Arrest. BioMed Research International, 2020, 9259852.
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