Total RNA was isolated from 0.02 g of tissue using the Ribopure Kit (Ambion, UK) according to the manufacturers’ instructions. Contaminating DNA was removed by On-column PureLink® DNase I treatment (Ambion). The quantity, quality and integrity of the RNA samples were determined using a NanoDrop ND-1000 spectrophotometer (Labtech International Ltd.) and Agilent 2200 TapeStation system (Agilent Technologies); all had an RNA Integrity Number of >7.3. cDNA synthesis from 1 μg RNA was by SuperScript™ II Reverse Transcription Kit (Invitrogen, UK) using oligo-dT(15) primer and RNaseOUT (Invitrogen).
On column purelink dnase 1 treatment
Manufactured by Thermo Fisher Scientific
On-column PureLink® DNase I treatment is a lab equipment product designed to remove genomic DNA contamination from RNA samples during the purification process. It provides an efficient and convenient method to eliminate DNA without the need for additional cleanup steps.
Automatically generated - may contain errors
Lab products found in correlation
Ribopure kit, by Thermo Fisher Scientific (2 mentions)
Agilent 2200 tapestation system, by Agilent Technologies (2 mentions)
Superscript 2 reverse transcription kit, by Thermo Fisher Scientific (1 mentions)
Rnaseout, by Thermo Fisher Scientific (1 mentions)
Nanodrop nd 1000 spectrophotometer, by Agilent Technologies (1 mentions)
Rnalater, by Thermo Fisher Scientific (1 mentions)
2 protocols using on column purelink dnase 1 treatment
1
Total RNA Isolation and cDNA Synthesis
2
Isolation of High-Quality RNA from Abomasal Lymph Nodes
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Abomasal (gastric) lymph nodes (ALN) were removed immediately post mortem and stored at –80˚C in RNAlater (Ambion, UK). Total RNA was isolated using the Ribopure Kit (Ambion) according to the manufacturers’ instructions. Contaminating DNA was removed by On-column PureLink® DNase I treatment (Ambion). The quantity, quality and integrity of the RNA samples were determined using a NanoDrop ND-1000 spectrophotometer and Agilent 2200 TapeStation system; all had an RNA Integrity Number of > 7.5.
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