For Py-GC/MS measurements, a combination of an EGA/PY-3030D multi-shot pyrolyzer (Frontier Lab, Fukushima, Japan), two GC 2010 Plus gas chromatographs with two fused silica capillary columns (Ultra Alloy columns: UA5-30M-0.25F and UACW-20M-0.25F; length of 30 m and 20 m respectively, inside diameter 0.25 mm, film thickness 0.25 μm) and a GCMS-QP2010 Ultra mass spectrometer (Shimadzu, Kyoto, Japan) was used. For the measurement the Shimadzu software MDGC Analysis GC solution 2.41.00. SU1 was used, evaluation was performed with Shimadzu GC/MS solution 2.72.70 (link) The lignin sample (0.5 mg) was placed in a stainless-steel pyrolysis crucible (PY1-EC50F) and pyrolyzed at 550 °C. For GC separation, a temperature program (1 min at 75 °C, then 280 °C for 25 min with a heating rate of 7 °C min−1) was used for the first column, the second one was isothermally tempered at 200 °C. The carrier gas used was helium 4.0 (Westfalen AG, Münster, Germany). For the MS detection, ionization was carried out via electron impact (EI) at 70 eV. Ions were detected by their mass/charge ratio (m/z); the measuring range was 35–740 u.
Gcms qp2010 ultra mass spectrometer
Manufactured by Shimadzu
Sourced in Japan
The GCMS-QP2010 Ultra is a high-performance gas chromatography-mass spectrometry (GC-MS) system. It is designed for the analysis of a wide range of organic compounds. The GCMS-QP2010 Ultra combines a gas chromatograph with a quadrupole mass spectrometer to provide rapid and accurate identification and quantification of sample components.
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9 protocols using gcms qp2010 ultra mass spectrometer
1
Py-GC/MS Analysis of Lignin
2
Quantifying Short-Chain Fatty Acids in Serum
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Blood samples were harvested from mice, and the serum was used for measurement of SCFA concentration. Samples were prepared as previously described (Fellows et al., 2018 (link)). Chromatographic analyses were performed using a GCMS-QP2010 Ultra mass spectrometer (Shimadzu; Thermo Fisher Scientific) and a 30 m × 0.25 mm fused-silica capillary Stabilwax column (Restek Corp.) coated with 0.25 µm polyethylene glycol. Samples (100 µl) were injected at 250°C using a 25:1 split ratio. High-grade pure helium was used as carrier gas at 1.0 ml/min constant flow. Mass conditions were as follows: ionization voltage, 70 eV; ion source temperature, 200°C; full scan mode, 35–500 m/z with 0.2-s scan velocity. The runtime was 11.95 min.
3
GC-MS Analysis of Crude Extracts
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To investigate the chemical fingerprinting of different crude extracts, the GC-MS analysis was performed using the Shimadzu GC-2010 Plus apparatus gas chromatograph was equipped with Shimadzu GCMS-QP2010 Ultra mass spectrometer and used Rxi 5MS steel column (30 mm × 0.25 mm × 0.25 µm). The carrier gas used was helium at constant pressure (37.1 kPa), with the oven temperature programmed from 50 °C and raised at 3 °C/min to a final temperature of 300 °C (hold time 10 min). The injector temperature was 250 °C, with an injection volume of 1 µL. The ion source temperature was 250 °C at the interface of 250 °C. The solvent cut time was 3 min and the total run time was 93 min. A continuous flow rate of 1 mL/min was employed for the carrier gas (helium) before a 1 mL sample was injected. In full scan mode, the mass spectral scan range was adjusted from 40 to 700 (m/z). The chemicals were identified using the National Institute of Standards and Technology (NIST) database. Their retention index was matched to those of legitimate samples deposited on Wiley. This was similar to the strategy employed by [61 (link)] with some modifications.
4
Spectroscopic Characterization of Organic Compounds
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The NMR (1H,
400 MHz and 13C, 100 MHz) spectra were recorded on a Bruker
AVANCE II spectrometer in DMSO-d6 using
tetramethylsilane as an internal standard. The 13C NMR
spectra were recorded in a mode of attached proton test with full
proton decoupling. The mass spectra (EI) were obtained by means of
a SHIMADZU GCMS-QP2010 Ultra mass spectrometer. The infrared spectra
were recorded on a Bruker Alpha FT-IR spectrometer. The elemental
analysis was fulfilled on a CHNS/O analyzer. The reactions course
was monitored by thin-layer chromatography (0.25 mm silica gel plates,
Merck 60F 254). Column chromatography was carried out on 220–440
mesh silica gel (60, 0.035–0.070 mm).
400 MHz and 13C, 100 MHz) spectra were recorded on a Bruker
AVANCE II spectrometer in DMSO-d6 using
tetramethylsilane as an internal standard. The 13C NMR
spectra were recorded in a mode of attached proton test with full
proton decoupling. The mass spectra (EI) were obtained by means of
a SHIMADZU GCMS-QP2010 Ultra mass spectrometer. The infrared spectra
were recorded on a Bruker Alpha FT-IR spectrometer. The elemental
analysis was fulfilled on a CHNS/O analyzer. The reactions course
was monitored by thin-layer chromatography (0.25 mm silica gel plates,
Merck 60F 254). Column chromatography was carried out on 220–440
mesh silica gel (60, 0.035–0.070 mm).
5
GC-MS Analysis of Volatile Compounds
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Analyses were performed on a chromatograph Shimadzu GC-2010 PLUS (Shimadzu, Kyoto, Japan) interfaced to a Shimadzu GC-MS-QP2010 ULTRA mass spectrometer (Shimadzu, Kyoto, Japan) and fitted with a capillary column Rxi-5MS (Restek, Bellefonte, PA, USA), (5%-phenyl)-methylpolisiloxane 33 m × 0.25 mm i.d., film thickness 0.25 µm). The conditions of chromatographic separation were the same as for the GC (FID) analysis. The temperature of the injector and detector was 250 °C. The flow rate of carrier gas (helium) was 1 mL/min, split 1:20. At least 2 repetitions (n ≥ 2) per analysis were performed. The temperature of ion source was 220 °C. Mass spectra in electron mode were generated at 70 eV, 0.97 scans/second, mass range 33–400 m/z.
6
GC-MS Analysis of Black Mulberry Seed Oil
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Before chemical analysis of the black mulberry seed oil, fatty acids were converted to methyl esters, according to Tadic et al. [28 (link)]. The chemical composition of the seed oil was analyzed using the GC and GC/MS technique according to the method by Tadic et al. [28 (link)]. A Shimadzu GCMSQP2010 ultra mass spectrometer fitted with a flame ionic detector and coupled with a GC2010 gas chromatograph was used for GC/MS analyses. Separation was performed using an InertCap5 capillary column (60.0 m × 0.25 mm × 0.25 µm). Helium (He), at a split ratio of 1:5 and a linear velocity of 35.2 cm/s, was used as a carrier gas. The temperature of the ion source was 200 °C, the injector temperature was 250 °C and the detector temperature was 300 °C, while the column temperature was linearly programmed from 40 to 260 °C (at a rate of 4 °C/min), from 260 to 310 °C (at a rate of 10 °C/min) and, after reaching 310 °C, kept isothermally for 10 min. Derivatized samples were dissolved in the methylene chloride and injected in an amount of 1 µL.
7
GC-MS Analysis of Organic Compounds
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Analyses were performed on a Shimadzu GC-2010 PLUS chromatograph (Shimadzu, Kyoto, Japan) interfaced w a Shimadzu GC-MS-QP2010 ULTRA mass spectrometer (Shimadzu, Kyoto, Japan) and fitted with a capillary column Rxi-5MS (Restek, Bellefonte, PA, USA), (5%-phenyl)-methylpolysiloxane 33 m × 0.25 mm i.d., film thickness of 0.25 µm).
The conditions of chromatographic separation were the same as those for GC (FID) analysis. The temperature of the injector and detector was 250 °C. The flow rate of the carrier gas (helium) was 1 mL/min, split 1:20. At least 2 replicates (n ≥ 2) per analysis were performed. The temperature of the ion source was 220 °C. Mass spectra in the electron mode were generated at 70 eV, with 0.97 scans/second and a mass range of 33–400 m/z.
The conditions of chromatographic separation were the same as those for GC (FID) analysis. The temperature of the injector and detector was 250 °C. The flow rate of the carrier gas (helium) was 1 mL/min, split 1:20. At least 2 replicates (n ≥ 2) per analysis were performed. The temperature of the ion source was 220 °C. Mass spectra in the electron mode were generated at 70 eV, with 0.97 scans/second and a mass range of 33–400 m/z.
8
GC-MS Analysis of Volatile Compounds
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Analyses were performed on a chromatograph Shimadzu GC-2010 PLUS (Shimadzu, Kyoto, Japan) interfaced to a Shimadzu GC-MS-QP2010 ULTRA mass spectrometer (Shimadzu, Kyoto, Japan) and fitted with a capillary column Rxi-5 MS (Restek, Bellefonte, PA, USA), (5%-phenyl)-methylpolysiloxane 33 m × 0.25 mm i.d., film thickness 0.25 µm).
The conditions of chromatographic separation were the same as for GC (FID) analysis. The temperature of the injector and detector was 250 °C. The flow rate of carrier gas (helium) was 1 mL/min, split 1:20. At least 2 replicates per analysis were performed. The temperature of ion source was 220 °C. Mass spectra in electron mode were generated at 70 eV, 0.97 scans/second, mass range 33–400 m/z.
The conditions of chromatographic separation were the same as for GC (FID) analysis. The temperature of the injector and detector was 250 °C. The flow rate of carrier gas (helium) was 1 mL/min, split 1:20. At least 2 replicates per analysis were performed. The temperature of ion source was 220 °C. Mass spectra in electron mode were generated at 70 eV, 0.97 scans/second, mass range 33–400 m/z.
9
GC/MS Analysis of Essential Oil and Hydrolat
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The chemical composition of the essential oil and hydrolat extraction parties was analyzed using GC/MS technique. GC/MS analyses were performed on a Shimadzu GCMS-QP2010 ultra mass spectrometer fitted with a flame ionic detector and coupled with a GC2010 gas chromatograph. The InertCap5 capillary column (60.0 m×0.25 mm×0.25 µm) was used for separation. Helium (He), at a split ratio of 1:5 and a linear velocity of 35.2 cm/s was used as a carrier gas. Initially, the oven temperature was 60 °C, which was held for 4 min, then increased to 280 °C at a rate of 4 °C/min, and held for 10 min. The injector and detector temperatures were 250 °C and 300 °C, respectively. The ion source temperature was 200 °C. The identification of the constituents was performed by comparing their mass spectra and retention indices (RIs) with those obtained from authentic samples and/or listed in the NIST/Wiley mass-spectra libraries, using different types of search (PBM/NIST/AMDIS) and available literature data (Adams, 2007) .
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