Cell lysates were prepared in RIPA buffer and immunoblotting was performed as previously described (19 (link)). Nuclear fractionation was performed using the REAP cellular fractionation method, as described in (35 (link)). Antibodies used were as follows: SPHK1 (Bethyl Laboratories, Montgomery, TX), SGPL1 and HIF1α (Abcam, Cambridge, MA), HIF2α (Novus Biologicals, Littleton, CO), phospho-AKT (S473), AKT, SOX2, phospho-AMPK (T172), AMPK, c-MYC, OCT-1, PKM1/2, β-Tubulin, Histone H3, cleaved caspase 3, Snail, V5-tag and β-Actin (Cell Signaling Technology, Beverly, MA).
Oct 1
Manufactured by Cell Signaling Technology
The OCT-1 is a lab equipment product that is used for optical coherence tomography (OCT) imaging. It provides high-resolution, cross-sectional imaging of biological tissues. The core function of the OCT-1 is to capture and process light reflections from internal tissue structures to generate detailed images.
Automatically generated - may contain errors
Lab products found in correlation
β actin, by Cell Signaling Technology (2 mentions)
Pkm1 2, by Cell Signaling Technology (1 mentions)
C myc, by Cell Signaling Technology (1 mentions)
Histone h3, by Cell Signaling Technology (1 mentions)
Snail, by Cell Signaling Technology (1 mentions)
V5 tag, by Cell Signaling Technology (1 mentions)
Phospho ampk, by Cell Signaling Technology (1 mentions)
β tubulin, by Cell Signaling Technology (1 mentions)
Phospho akt, by Cell Signaling Technology (1 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Hif 2α, by Novus Biologicals (1 mentions)
Ubiquitin, by Cell Signaling Technology (1 mentions)
P creb, by Cell Signaling Technology (1 mentions)
Pe isotype control, by BioLegend (1 mentions)
P src, by Cell Signaling Technology (1 mentions)
Nfat1, by Cell Signaling Technology (1 mentions)
Methamphetamine hydrochloride, by Merck Group (1 mentions)
Gw182, by Santa Cruz Biotechnology (1 mentions)
P nf κb, by Cell Signaling Technology (1 mentions)
Fitc isotype control, by BioLegend (1 mentions)
2 protocols using oct 1
1
Immunoblotting and Nuclear Fractionation
2
HIV-1 BaL Infection Pathway Analysis
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HIV-1 BaL was obtained from the NIH AIDS Research and Reference Reagent Program, National Institute of Allergy and Infectious Diseases, NIH. The HIV-1 BaL strain has been shown to efficiently infect, via the CCR5 co-receptor, and replicate within CD4+ T-cells49 (link). Argonaute-1, p-NFκB, NFκB, p-ERK, p-Src, p-Akt, NFAT1, p-CREB, CREB, Oct-1, Ubiquitin, and β-Actin antibodies were obtained from Cell Signaling Technology (Danvers, MA). GW182, D1DR, D2DR, D3DR, D4DR, Sigma-1 Receptor, and GAPDH antibodies were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). FITC-conjugated p24 GAG (6604665) antibody was obtained from Beckman Coulter, Inc. (Brea, CA). PE-conjugated CD45RO, PE-conjugated HLA-DR, FITC-conjugated CD45RA, FITC-conjugated CD25, FITC-conjugated CD69, PE isotype control, and FITC isotype control antibodies were purchased from Biolegend (San Diego, CA). p-NFAT1 antibody was obtained from Thermo Fisher Scientific (Waltham, MA). Fluo-4, AM was purchased from Invitrogen (Carlsbad, CA). Methamphetamine hydrochloride and sigma-1 receptor inhibitor (BD1047) were purchased from Sigma Aldrich (St. Louis, MO).
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