Phytohormone contents were measured by the method described previously [37] . Extracted samples were subjected to measurement on a triple quadrupole LC-MS/MS 6410 (Agilent Technologies, USA) equipped with a Zorbax SB-C18 column [2.1 mm id×50 mm, (1.8 µm), Agilent Technologies]. Hormone amounts were calculated from the ratio of endogenous hormone peak and known amount of internal standards spike, and related to actual fresh mass of the samples used for extraction.
Triple quadrupole lc ms ms 6410
Manufactured by Agilent Technologies
Sourced in United States
The Agilent Triple Quadrupole LC-MS/MS 6410 is a high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. It is designed for the sensitive and selective analysis of a wide range of analytes in complex matrices. The system combines the separation capabilities of liquid chromatography with the precise mass detection and quantification of a triple quadrupole mass spectrometer.
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3 protocols using triple quadrupole lc ms ms 6410
1
Phytohormone Quantification via LC-MS
2
Quantifying Phytohormones via LC-MS/MS
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Phytohormone contents were determined using a method described previously by Kiba et al. (2012) (link). Extracted samples were measured on a triple-quadrupole LC-MS/MS 6410 (Agilent Technologies) equipped with a Zorbax SB-C18 column (2.1 mm id×50 mm, 1.8 µm; Agilent Technologies). The amounts of hormones were calculated from the ratios of the endogenous hormone peaks and the known amounts of internal standards, and expressed in relation to the fresh weight of the samples used for extraction.
3
Phytohormone Quantification by LC-MS/MS
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Phytohormone methods followed standard procedure described in Fukumoto et al. (2013) (link).
Phytohormone contents were measured on a triple quadrupole LC-MS/MS 6410 (Agilent Technologies, Santa Clara, CA, USA) equipped with a Zorbax SB-C18 column [2.1 mm id × 50 mm, (1.8 µm), Agilent Technologies].
Phytohormone contents were measured on a triple quadrupole LC-MS/MS 6410 (Agilent Technologies, Santa Clara, CA, USA) equipped with a Zorbax SB-C18 column [2.1 mm id × 50 mm, (1.8 µm), Agilent Technologies].
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