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Cd11a clone m17 4

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CD11a (clone M17/4) is a monoclonal antibody that recognizes the alpha subunit of the leukocyte-associated antigen-1 (LFA-1) integrin. LFA-1 is a cell surface receptor involved in cell-cell adhesion and is expressed on various leukocyte populations. The CD11a (clone M17/4) antibody can be used for the identification and analysis of cells expressing the LFA-1 integrin.

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Lab products found in correlation

Cd44 clone im7, by BioLegend (2 mentions) Tnfα clone mp6 xt22, by BioLegend (1 mentions) Ifnγ clone xmg1, by BioLegend (1 mentions) Cd8 clone 53 6, by Thermo Fisher Scientific (1 mentions) Pd 1 clone j43, by Thermo Fisher Scientific (1 mentions) Thy1.1 clone his51, by Thermo Fisher Scientific (1 mentions) Cytofix cytoperm, by BD (1 mentions) Lsr 2, by BD (1 mentions) Monensin, by BD (1 mentions) Pd 1 clone rmp1 30, by BioLegend (1 mentions) Anti mouse ifnγ clone xmg1, by Thermo Fisher Scientific (1 mentions) Anti cd44, by BD (1 mentions) Anti cd4, by BD (1 mentions) Anti cxcr5 clone 2g 8, by BD (1 mentions) Anti tbet clone 4b10, by BioLegend (1 mentions) Anti cxcr3, by BD (1 mentions) Cxcr3 clone cxcr3 173, by BioLegend (1 mentions) Anti tnf clone mp6 xt22, by Thermo Fisher Scientific (1 mentions) Anti il 2 clone jes6 5h4, by Thermo Fisher Scientific (1 mentions) Cd25 clone pc61, by BioLegend (1 mentions)

Close spelling variants of this product

CD11a (7 citations) CD11a (M17/4; (3 citations) Anti- mouse CD11a (clone M17/4) (2 citations)

4 protocols using cd11a clone m17 4

1

Characterization of LCMV-specific CD8+ T cells

Cited 1 time
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The following mAb were purchased from eBioscience or Biolegend and used in an appropriate combination of fluorochromes: CD8 (clone 53-6.7; eBioscience), PD-1 (clone J43; eBioscience), LAG-3 (clone eBioC9B7W; eBioscience), 2B4 (clone eBio244F4; eBioscience), CD11a (clone M17/4; eBioscience), Thy1.1 (clone HIS51; eBioscience), IFNγ (clone XMG1.2; Biolegend) and TNFα (clone MP6-XT22; Biolegend). All LCMV-specific peptides were synthesized by Bio-Synthesis Inc (Bio-Synthesis, Louisville, TX): GP276-286 (SGVENPGGYCL) and GP33-41 (KAVYNFATM) (52 (link)). p:MHC class I tetramer H-2Db GP276 and GP33 were made and used as previously described (25 (link), 48 (link)).
Condotta S.A., Khan S.H., Rai D., Griffith T.S, & Badovinac V.P. (2015). Poly-microbial sepsis increases susceptibility to chronic viral infection and exacerbates CD8+ T cell exhaustion. Journal of immunology (Baltimore, Md. : 1950), 195(1), 116-125.
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2

Multi-parameter T and B Cell Analysis

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T cells were stained with H2-Kb/GP61–80 tetramers (NIH) and antibodies against mouse CD4 (clone GK1.5, Biolegend), CD11a (clone M17/4, eBioscience), CD25 (clone PC61, Biolegend), CD44 (clone IM7, Tonbo), Ly6C (clone HK1.4, Biolegend), CXCR3 (clone CXCR3-173, Biolegend), and PD-1 (clone RMP1-30, Biolegend). B cells were stained with CD19 (clone 6D5, Tonbo), T and B cell activation antigen (clone GL-7, BD Biosciences), CD95 (clone Jo2, BD Biosciences), IgD (clone 11-26c.2a, BD Biosciences), and IgM (clone RMM-1, eBioscience). Cells were stimulated with either GP61–80 peptide antigen or PMA and ionomycin in the presence of monensin, permeabilized with cytofix/cytoperm (BD Bioscience), and stained with anti-mouse IFN-γ (clone XMG1.2, eBioscience), anti-IL-2 (clone JES6-5H4, eBioscience), and anti-TNF (clone MP6-XT22, eBioscience). For Tfh analyses, cells were stained with anti-CXCR5 (clone 2G8, BD Biosciences) followed by staining with fluorochrome-conjugated anti-CD4, anti-CD44, anti-Ly6C, anti-CXCR3, and anti-PD-1. Anti-T-bet (clone 4B-10, Biolegend) and anti-Bcl-6 (clone K112-91, BD Biosciences) were applied after fixation and permeabilization of cells using FoxP3/Transcription Factor Staining Buffer Kits (Tonbo). Data were acquired using either Stratedigm S1200Ex or BD LSR II flow cytometers and analyzed using FlowJo software (Tree Star, Inc.).
Zander R.A., Vijay R., Pack A.D., Guthmiller J.J., Graham A.C., Lindner S.E., Vaughan A.M., Kappe S.H, & Butler N.S. (2017). Th1-like Plasmodium-specific memory CD4+ T cells unexpectedly support humoral immunity. Cell reports, 21(7), 1839-1852.
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3

Multiparametric Flow Cytometry Analysis

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Fluorescently labeled monoclonal antibodies against the following mouse antigens were used: CD3 (clone 145–2 C11, BD Biosciences), CD4 (clone RM4-5, BioLegend), CD8a (clone 53–6.7, BioLegend), CD8b (clone YTS156.7.7, BioLegend), CD11a (clone M17/4, eBioscience), CD127 (clone A7R34, Thermo Fisher), KLRG-1 (clone 2F1, Thermo Fisher), CD44 (clone IM7, BioLegend), CD49a (clone Ha31/8, BD Biosciences), CD62L (clone MEL-14, BioLegend), CD69 (clone H1.2F3, BD Biosciences), CD38 (clone 90, Thermo Fisher) and CD103 (clone 2E7, Thermo Fisher). Cells were stained according to our previously published protocol.33 (link) 7-AAD (A1310, Invitrogen) staining was used to exclude dead cells. E7-specific CD8+ T cells were quantified using MHC class I tetramers for the RAHYNIVTF epitope. Flow cytometric acquisition was performed on a BD Fortessa flow cytometer (BD Biosciences).
van der Gracht E.T., Schoonderwoerd M.J., van Duikeren S., Yilmaz A.N., Behr F.M., Colston J.M., Lee L.N., Yagita H., van Gisbergen K.P., Hawinkels L.J., Koning F., Klenerman P, & Arens R. (2020). Adenoviral vaccines promote protective tissue-resident memory T cell populations against cancer. Journal for Immunotherapy of Cancer, 8(2), e001133.
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4

Comprehensive Flow Cytometry Antibody Panel

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For flow cytometry, Abs to CD16/CD32 (FcγRII/III; Clone 2.4G2), Ly6C (Clone AL-21), CD80 (Clone 16–10A1), IL-2 (Clone JES6–5H4), CD4 (Clone RM4–5), TNF (Clone MP6-XT22), CD62L (Clone MEL-14), NK1.1 (Clone PK136), B220 (Clone RA3–6B2), CD11b (Clone M1/70) and CD3ε (Clone 17A2) were purchased from BD PharMingen; IFNγ (Clone XMG1.2), CD49d (Clone R1–2), CD3ε (Clone 145–2C11), CD115, CD44 (Clone 1M7), CD86 (Clone GL-1) and I-A/I-E (Clone M5/114.15.2) were purchased from BioLegend; and CD11c (Clone N418), CD69 (Clone H1.2F3), CD3ε (Clone 145–2C11), Siglec H (Clone eBio440c), CD3ε (Clone 17A2), CD11b (Clone M1/70), Ly49H (Clone 3D10), Ly6C/6G (Gr-1; Clone RB6–8C5), CD11a (Clone M17/4) and CD28 (Clone 37.51) were purchased from eBioscience. The following Abs were purchased from Invitrogen: CD8α (Clone 5H10), F4/80 (Clone BM8) and CD45 (Clone 30-F11). For ELISA, purified mouse IgM, IgG1, IgG2b and IgG2c, and polyclonal nonconjugated and alkaline phosphatase (AP)-conjugated goat anti-mouse Ig isotypes were purchased from Southern Biotech.
Feng Y., Livingston-Rosanoff D., Roback L., Sundararajan A., Speck S.H., Mocarski E.S, & Daley-Bauer L.P. (2018). Remarkably Robust Antiviral Immune Response Despite Combined Deficiency in Caspase-8 and RIPK3. Journal of immunology (Baltimore, Md. : 1950), 201(8), 2244-2255.
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