Csb e14247r

The levels of LPS, IL-6, TNF-α, IL-12, 8-Oxo-dG, ATP, and Citrate synthase (CS) were measured in the plasma and myocardial tissues of rats. LPS (CSB-E14247R, CUSABIO), IL-6 (CSB-E04640R, CUSABIO), TNF-α (CSB-E11987R, CUSABIO), IL-12 (CSB-E07362R, CUSABIO), 8-oxo-dG (ML059056, MLBIO), ATP (A095-1-1, Nanjing Jiancheng Bioengineering Institute), and CS (A108, Nanjing Jiancheng Bioengineering Institute) assay kit were applied to detect LPS, IL-6, TNF-α, IL-12, 8-oxo-dG, ATP, and CS levels according to the introductions. The concentration of LPS, IL-6, TNF-α, IL-12, 8-oxo-dG, ATP, and CS was calculated by using the standard curve provided by DYNATECHMR7000 micrometer.

Blood samples were collected in blood collection tubes coated with K2EDTA (BD, Cat# 36 784), and centrifuged for 15 min at 4 °C, 12 000 × g. Plasma GLP‐1, ghrelin, leptin, and LPS levels were determined by using corresponding ELISA kits CSB‐E08117r, CSB‐E09816 and CSB‐E07433r, and CSB‐E14247r (CUSABIO), respectively, according to the manufacturer's instructions.