Oasis hlb 3 cm3 60 mg

We determined urinary OPs and PYR insecticide levels using a method from a previous study with some modi cations (Olsson et al. 2004 (link)). Before the analysis, 2 ml of samples brought to room temperature were taken into test tubes and 25 µl of the isotope labeled standarts of analyzed pesticides we prepared were added to it. 10 µl of β-glucuronidase enzyme was added to hydrolyze sulfate-bound or glucuronide metabolites. 1 mL of 0.2M acetate buffer was added to each urine sample. Before extracted by solid-phase extraction (SPE) (Oasis HLB 3 cm 3 /60 mg, Waters, MA, USA ), all samples were incubated at 37°C for 17 hours. The SPE cartridge was conditioned with 1mL of methanol and then 1mL of 1% acetic acid. Sample was loaded into the cartridge and slowly passed through the SPE cartridge. To reduce interfering substances, the cartridges were washed with 1mL of water with 5% methanol with 1% acetic acid. SPE cartridges were dried under vacuum for 30 seconds. After elution with 1.5 mL of methanol, 2 mL of acetonitrile was added to it, and after it was evaporated to dryness, it was dissolved with 50 µL of acetonitrile and transferred to the LC/MS-MS device for analysis by taking it into vials with insert.
Chromatographic and mass spectrometric conditions