Fasting peripheral venous blood samples were collected at study inclusion in all participants. Following centrifugation (2,200 rpm, 20 minutes, 4°C), ethylene-diamine-tetra-acetic acid-plasma samples were stored in multiple aliquots at −20°C until further analysis. Plasma oxLDL (Mercodia, Uppsala, Sweden) was determined by commercially available enzyme-linked immunosorbent assay (ELISA). Intra- and interassay coefficients of variation for oxLDL were 6.8% and 7.1%, respectively. Determination of CRP was performed by immunonephelometric technique (Dade Behring BNII Nephelopmeter, Marburg, Germany) with detection limit of 0.2 mg/L and a coefficient of variation of 4.7%.
Plasma oxldl
Manufactured by Mercodia
Sourced in Sweden, Germany
Plasma oxLDL is a laboratory equipment product that measures the concentration of oxidized low-density lipoprotein (oxLDL) in plasma samples. It provides quantitative data on the level of oxidized LDL, which is a biomarker associated with cardiovascular health.
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Lab products found in correlation
2 protocols using plasma oxldl
1
Plasma oxLDL and CRP Determination
2
Serum LOX-1 and Plasma oxLDL Measurement
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Non‐fasting venous blood was collected between 2017 and 2022 in 7.5 mL S‐Monovette Serum‐Gel (Sarstedt, Germany) containing a clotting activator/gel. Blood samples were centrifuged at 1.500g for 12 minutes and the serum was stored in aliquots at −80°C. Serum LOX‐1 (EHOLR1, Thermo Fisher Scientific, Germany) and plasma oxLDL (#10–1143‐01, Mercodia, Uppsala, Sweden) were determined in duplicates by ELISA according to the manufacturer's instructions. If necessary, serum was diluted 1:2 or 1:5 and 2 internal controls were run on every plate. The intervariation coefficient was 14.2% for plates 1–5, and 20% for plates 6–7, respectively. The mean variation between all duplicates was 1±4%.
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