Emtricitabine 13c 15n2

The following reagents were obtained through the National Institutes of Health (NIH) AIDS Reagent Program, Division of AIDS, National Institute of Allergy and Infectious Diseases (NIAID), tenofovir, emtricitabine, and dolutegravir. Tenofovir-d6 and emtricitabine-¹³C ¹⁵N2 were obtained from Toronto Research Chemical Inc. (Ontario, Canada). Dolutegravir-d5 was purchased from BDG Synthesis (Wellington, NZ). Human brain microvascular endothelial cells, hCMEC/D3, were generously provided by Dr. Babette Weksler of Weill Cornell Medical College, Cornell University, New York, NY. These cells were grown in EBM-2 medium (Lonza, Walkersville, MD) and supplemented as described previously [29 (link)] and all experiments were used between passage number 28 and 32. Primary human pericytes and astrocytes, as well as the appropriate culture medium were purchased from ScienCell, Inc. (Carlsbad, CA). Primary human microglia and medium were obtained from Celprogen, Inc. (Torrance, CA). Primary human brain endothelial cells and medium were purchased from Cell Systems, Inc. (Kirkland, WA). Recombinant HIV-1 Tat was obtained from ImmunoDX, Inc. (Woburn, MA). All other compounds were purchased from Fisher Scientific.