Picropodophyllin

Manufactured by Selleck Chemicals

Sourced in United States

Picropodophyllin is a chemical compound used in laboratory settings. It is a natural product isolated from the roots of the mayapple plant (Podophyllum peltatum). The compound has a molecular formula of C22H22O8 and is commonly employed as a research tool in various scientific investigations.

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Lab products found in correlation

8 protocols using picropodophyllin

Rehmannia glutinosa Extraction and Compound Procurement

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RR was purchased from Shanghai Dekang Pharmaceutical Co. Ltd. (Shanghai, China), and identified as the root of Rehmannia glutinosa Libosch by Professor Qiao-Yan Zhang. The voucher specimen (No. 2015007) was deposited in the herbarium of the Department of Pharmacognosy of the Second Military Medical University (Shanghai, China). RR was extracted with 8-fold water x 3. Then, the mixture was filtered and the filtrate was evaporated under reduced pressure. CAT and ACT were obtained from Yuanye Biological Technology Co. Ltd. (Shanghai, China). ECH was obtained from Chenguang Biological Technology Co. Ltd. (Baoji, China). Metformin hydrochloride tablets were purchased from Shiguibao Pharmaceutical Co. Ltd. (Shanghai, China). Alendronate sodium tablets were obtained from MSD pharmaceutical Co. Ltd. (Hangzhou, China). Streptozotocin was obtained from Sigma-Aldrich (St Louis, MO, USA). Picropodophyllin was obtained from Selleck (Shanghai, China). DPD, OCN, and IGF-1 ELISA kits were purchased from Xinyu Biological Technology Co. Ltd. (Shanghai, China). ALP and TRAP kits were purchased from Jiancheng Bioengineering Institute (Nanjing, China). The BCA Kit was purchased from Beyotime Institute of Biotechnology (Shanghai, China).

Gong W., Zhang N., Cheng G., Zhang Q., He Y., Shen Y., Zhang Q., Zhu B., Zhang Q, & Qin L. (2019). Rehmannia glutinosa Libosch Extracts Prevent Bone Loss and Architectural Deterioration and Enhance Osteoblastic Bone Formation by Regulating the IGF-1/PI3K/mTOR Pathway in Streptozotocin-Induced Diabetic Rats. International Journal of Molecular Sciences, 20(16), 3964.

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Functional Regulation of miRNA Targets

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Hsa-miR-483-5p mimic, YM00473215-ADA, mimic control, YM00479902-ADA, inhibitors hsa-miR-675-5p, 427419-00, hsa-miR-675-3p, 427420-00, hsa-miR-483-5p, 427155-00, hsa-miR-483-3p, 427154-00, hsa-miR-204-5p, 426934-00, hsa-miR-135-5p, 426790-00, hsa-miR-10a-5p, 426651-00, hsa-miR-9-5p, 427460-00, hsa-miR-9-3p, 427461-00, Control B, 199021-00 are from Qiagen, Germantown, MD, USA. Ceritinib, S7083, linsitinib, S1091, entrectinib, S7998, GSK1904529A, S1093, picropodophyllin, S7668 were obtained from Selleck Chemicals, Houston, TX, USA; GSK1838705A, SML0995, was purchased from Millipore-Sigma, Burlington, MA, USA. The primers used for miRNA and mRNA quantification were miR-99b-5p, 4,427,975, miR-483-5p, 4,427,975, miR-675-5p, 4,427,975, IGF-2, 4,331,182, GAPDH, 4,331,182, ThermoFisher Scientific, Waltham, MA, USA.

Uhlmann E.J., Mackel C.E., Deforzh E., Rabinovsky R., Brastianos P.K., Varma H., Vega R.A, & Krichevsky A.M. (2023). Inhibition of the epigenetically activated miR-483-5p/IGF-2 pathway results in rapid loss of meningioma tumor cell viability. Journal of Neuro-Oncology, 162(1), 109-118.

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DLBCL Cell Line Culture and Inhibitors

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Human DLBCL cell lines (LY1, LY8, LY3, and Val) were routinely cultured in Iscove’s modified Dulbecco’s medium (IMDM) with 10% heat-inactivated fetal bovine serum (Gibco, MD, USA). The medium contained a 1% penicillin/streptomycin mixture and 2 mM glutamine. Verteporfin (VP; a YAP inhibitor, SML0534) was obtained from Sigma (MO, USA). Recombinant human IGF-1 was obtained from PeproTech (100-11, NJ, USA). Doxorubicin (S1208), AG1024 (an IGF-1R inhibitor, S1234), and picropodophyllin (PPP; an IGF-1R inhibitor, S7668) were purchased from Selleckchem (TX, USA).

Zhou X., Chen N., Xu H., Zhou X., Wang J., Fang X., Zhang Y., Li Y., Yang J, & Wang X. (2020). Regulation of Hippo-YAP signaling by insulin-like growth factor-1 receptor in the tumorigenesis of diffuse large B-cell lymphoma. Journal of Hematology & Oncology, 13, 77.

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Lacrimal Gland Explant Culture Assay

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Lacrimal gland explant cultures were prepared as previously described (28 (link)). Briefly, the whole eye, together with the adjacent ectoderm and mesenchyme, was dissected from E13.5 mouse embryos carrying Le-Cre. The explants were cultured on a membrane filter (HAWP01300, 0.45-μm pore, Millipore) and covered by Matrigel/DMEM/F12 mixture in the presence or absence of growth factors. Bovine serum albumin (BSA) or EGF (final concentration, 100 ng/ml) were first diluted in DMEM/F12 before mixed with Matrigel in 2:1 ratio. Ten microliter of the mixture was added on top of each explant. In each experiment, one side of embryo was treated with BSA and the other side with EGF. For inhibitor study, IGF inhibitor Picropodophyllin (10 μM; #S7668, Selleckchem), PI3K inhibitor LY294002 (1 mM) and mTOR inhibitor Torin (0.5 μM) were added to the media. After 2 days of incubation at 37°C with 5% CO₂, the explants were examined for GFP-expressing lacrimal gland buds.

Wang Q., Tao C., Hannan A., Yoon S., Min X., Peregrin J., Qu X., Li H., Yu H., Zhao J, & Zhang X. (2021). Lacrimal gland budding requires PI3K-dependent suppression of EGF signaling. Science Advances, 7(27), eabf1068.

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Insulin Receptor and Signaling Pathways

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Insulin and pork gelatin were obtained from Sigma‐Aldrich (St. Louis, MO). Antibodies to the insulin receptor β (IRβ) subunit (C‐terminus) and hydroxy‐2‐naphthalenylmethylphosphonic acid trisacetoxymethyl (HNMPA‐(AM)₃), an insulin receptor tyrosine kinase inhibitor, were obtained from Abcam (Cambridge, MA). The remaining antibodies were purchased from Cell Signaling Technology (Danvers, MA). SB203580 (a p38 inhibitor), wortmannin (a PI3K inhibitor), LY294002 (a PI3K inhibitor) and its control LY303511, rapamycin (an mTOR/p70^s6 kinase inhibitor), PD98059 (a MEK1 inhibitor), SP600125 (a JNK inhibitor) and picropodophyllin (PPP, an IGF1R inhibitor) were obtained from Selleck Chemicals (Houston, TX).

Wang G., Yin L., Peng Y., Gao Y., Gao H., Zhang J., Lv N., Miao Y, & Lu Z. (2019). Insulin promotes invasion and migration of KRASG12D mutant HPNE cells by upregulating MMP‐2 gelatinolytic activity via ERK‐ and PI3K‐dependent signalling. Cell Proliferation, 52(3), e12575.

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GH-IGF1 Signaling in Lung Cells

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GH (#86900, Sigma-Aldrich, St. Louis, MO, USA), IGF1 (#I8779 Sigma-Aldrich, St. Louis, MO, USA), and picropodophyllin (IGF1-R inhibitor; #S7668 Selleckchem, München, Germany) were used to analyze the role of GH–IGF1 signaling in MLE-12 and pLFs. First, 0.5–1.0 × 10⁶ cells were seeded per 10 cm dish in serum-rich medium (2% FBS for MLE-12, 10% for pLF) and incubated under normal growth conditions for 24 h (5% CO₂ at 37 °C). Prior to stimulation with GH and IGF1, cells were starved for 12 h using serum-reduced medium (0.2% FBS for MLE-12, 1% for pLF). Subsequently, cells were stimulated with GH (100 nmol/L or 200 nmol/L) diluted in 10 mmol sodium bicarbonate, IGF1 (10 ng/mL or 100 ng/mL) diluted in dH₂O, and IGF1-R inhibitor (20 nM/mL) diluted in ethanol. Controls were treated with the respective vehicle. Six independent experiments were performed; for mRNA or protein isolation, cells were collected 24 h after stimulation.

Vohlen C., Mohr J., Fomenko A., Kuiper-Makris C., Grzembke T., Aydogmus R., Wilke R., Hirani D., Dötsch J, & Alejandre Alcazar M.A. (2021). Dynamic Regulation of GH–IGF1 Signaling in Injury and Recovery in Hyperoxia-Induced Neonatal Lung Injury. Cells, 10(11), 2947.

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Evaluation of Candidate Inhibitors

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YM-254890 was purchased from Focus Biomolecules (10–1590) and Wako Pure Chemical Industries (257–00631). Linsitinib (OSI-906) was purchased from Selleck Chemical (S1091) and LC Laboratories (L-5814). Picropodophyllin (PPP) and Alpelisib (BYL719) were purchased from Selleck Chemical. IGF1 was purchased from Abcam.

Lapadula D., Lam B., Terai M., Sugase T., Tanaka R., Farias E., Kadamb R., Lopez-Anton M., Heine C.C., Modasia B., Aguirre-Ghiso J.A., Aplin A.E., Sato T, & Benovic J.L. (2023). IGF1R inhibition enhances the therapeutic effects of Gq/11 inhibition in metastatic uveal melanoma progression. Molecular cancer therapeutics, 22(1), 63-74.

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Anticancer Agents Sourcing Protocol

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All the chemicals employed in this study were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Anticancer agents, vinorelbine ditartrate (S4269), picropodophyllin (S7668), pacritinib (S8057), and SKLB610 (S6526) were obtained from Selleck Chemicals (Houston, TX, USA).

Mo H.Y., Wei Q.Y., Zhong Q.H., Zhao X.Y., Guo D., Han J., Noracharttiyapot W., Visser L., van den Berg A., Xu Y.M, & Lau A.T. (2022). Cytochrome P450 27C1 Level Dictates Lung Cancer Tumorigenicity and Sensitivity towards Multiple Anticancer Agents and Its Potential Interplay with the IGF-1R/Akt/p53 Signaling Pathway. International Journal of Molecular Sciences, 23(14), 7853.

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