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Dimethylformamide dmf

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Dimethylformamide (DMF) is a clear, colorless, and volatile liquid commonly used as a solvent in various laboratory applications. It has a high boiling point and is miscible with water and many organic solvents. DMF is a versatile compound that serves as an effective medium for chemical reactions, extractions, and other analytical procedures in the laboratory setting.

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Lab products found in correlation

N methylmorpholine, by Merck Group (4 mentions) Acetic anhydride, by Thermo Fisher Scientific (2 mentions) Glacial acetic acid, by Thermo Fisher Scientific (2 mentions) Diisopropylethylamine, by Merck Group (1 mentions) N methylmorpholine nmm, by Merck Group (1 mentions)

5 protocols using dimethylformamide dmf

1

Solid-Phase Peptide Synthesis Protocol

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Fmoc-protected amino acids, piperidine, trifluoroacetic acid (TFA), 2-chlorotrityl chloride resin, 2 - (6 - chloro - 1H - benzotriazole - 1 - yl) - 1,1,3,3 - tetramethylaminium hexafluorophosphate (HCTU), and 1, 1-[bis(dimethylamino) methylene]-1H-1,2,3-triazolo[4,5-b] pyridinium 3-oxide hexafluorophosphate (HATU) were purchased from Advanced ChemTech (Louisville, KY). Diisopropylethylamine (DIEA), triisopropylsilane (TIPS), and N-methylmorpholine (NMM) were purchased from Sigma Aldrich (St. Louis, MO). Dimethylformamide (DMF) was purchased from Protein Technologies (Tucson, AZ). Dichloromethane (DCM), methanol (MeOH), and acetonitrile (CH3CN) were purchased from VWR International, LLC (Radnor, PA). 4-(Fmoc-2-aminoethyl)-6-dibenzofuranpropionic acid (Fmoc-DBF) was purchased from Chem-Implex International Inc. (Wood Dale, IL).
Dahal A., Parajuli P., Singh S.S., Shrestha L., Sonju J.J., Shrestha P., Chatzistamou I, & Jois S. (2022). Targeting protein–protein interaction for immunomodulation: A sunflower trypsin inhibitor analog peptidomimetic suppresses RA progression in CIA model. Journal of pharmacological sciences, 149(3), 124-138.
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2

Solid-Phase Peptide Synthesis Protocol

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Fmoc-protected amino acids, 2-(6-Chloro-1H-benzotriazole-1-yl)-1,−1,3,3-tetramethylaminium hexafluorophosphate (HCTU), trifluoroacetic acid (TFA), and rink amide SS resin were purchased from Advanced ChemTech, Louisville, Kentucky. Nα-Fmoc-Nδ-allyloxycarbonyl-l-ornithine (Fmoc-Orn[Aloc]-OH) was purchased from Chem-Impex International, Inc, Wood Dale, Illinois. 1-Hydroxy-6-(trifluoromethyl)benzotriazole (HOBt) and (Ethyl cyano[hydroxyimino]acetato)-tri-(1-pyrrolidinyl)-phosphonium (PyBOP) were purchased from Novabiochem, Billerica, Massachusetts. Dimethylformamide (DMF) was purchased from Protein Technologies, Tucson, Arizona. Diisopropylethylamine (DIEA), triisopropylsilane (TIPS), tetrakis(triphenylphosphine)palladium(0) (palladium), 5(6)-carboxyfluorescein (FAM), chloroform (CHCl3), methanol (MeOH), dichloromethane (DCM), and N-methylmorpholine (NMM) were purchased from Sigma-Aldrich, St. Louis, Missouri. Glacial acetic acid was purchased from Alfa Aesar, Ward Hill, Massachusetts. Acetic anhydride was purchased from Fisher Scientific, Fair Lawn, New Jersey.
Safa N., Anderson J.C., Vaithiyanathan M., Pettigrew J.H., Pappas G.A., Liu D., Gauthier T.J, & Melvin A.T. (2018). CPProtectides: Rapid uptake of well-folded β-hairpin peptides with enhanced resistance to intracellular degradation. Peptide science (Hoboken, N.J.), 111(2), e24092.
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3

Solid-Phase Peptide Synthesis Protocol

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Fmoc-protected amino acids, piperidine, trifluoroacetic acid (TFA), 2-chlorotrityl chloride resin, 2 - (6 - chloro - 1H - benzotriazole - 1 - yl) - 1,1,3,3 - tetramethylaminium hexafluorophosphate (HCTU), and 1, 1-[bis(dimethylamino) methylene]-1H-1,2,3-triazolo[4,5-b] pyridinium 3-oxide hexafluorophosphate (HATU) were purchased from Advanced ChemTech (Louisville, KY). Diisopropylethylamine (DIEA), triisopropylsilane (TIPS), and N-methylmorpholine (NMM) were purchased from Sigma Aldrich (St. Louis, MO). Dimethylformamide (DMF) was purchased from Protein Technologies (Tucson, AZ). Dichloromethane (DCM), methanol (MeOH), and acetonitrile (CH3CN) were purchased from VWR International, LLC (Radnor, PA). 4-(Fmoc-2-aminoethyl)-6-dibenzofuranpropionic acid (Fmoc-DBF) was purchased from Chem-Implex International Inc. (Wood Dale, IL).
Dahal A., Parajuli P., Singh S.S., Shrestha L., Sonju J.J., Shrestha P., Chatzistamou I, & Jois S. (2022). Targeting protein–protein interaction for immunomodulation: A sunflower trypsin inhibitor analog peptidomimetic suppresses RA progression in CIA model. Journal of pharmacological sciences, 149(3), 124-138.
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4

Peptide Synthesis with Fluorescent Labeling

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Fmoc-protected amino acids, 2-(6-Chloro-1H-benzotriazole-1-yl)-1, -1,3,3-tetramethylaminium hexafluorophosphate (HCTU), trifluoroacetic acid (TFA), and rink amide SS resin, were purchased from Advance ChemTech, Louisville, KY, USA. Nα-Fmoc-Nδ-allyloxycabonyl-L-ornithine (Fmoc-Orn[Aloc]-OH) was purchased from Chem-Impex International, Inc., Wood Dale, IL, USA. 1-Hydroxy-6-(trifluoromethyl) benzotriazole (HOBt) and (Ethyl cyano[hydroxyimino]acetato)-tri-(1-pyrrolidinyl)-phosphonium (PyBOP) were purchased from Novabiochem, Billerica, MA, USA. Dimethylformamide (DMF) was purchased from Protein Technologies, Tucson, AZ, USA. Diisopropylethylamine (DIEA), triisopropylsilane (TIPS), tetrakis(triphenylphosphine) palladium (0) (palladium), 5(6)-carboxyfluorescein (FAM), chloroform (CHCl3), methanol (MeOH), dichloromethane (DCM), and N-methylmorpholine (NMM) were purchased from Sigma-Aldrich, St. Louis, MO, USA. Glacial acetic acid was purchased from Alfa Aesar, Ward Hill, MA, USA. Acetic anhydride was purchased from Fisher Scientific, Fair Lawn, NJ, USA. Commercial peptide ARG (FAM-RRRRRRRRR) was purchased from AnaSpec, Fremont, CA, USA. All the salts used for the preparation of assay buffers were purchased from Sigma Aldrich, St. Louis, MO, USA.
Hymel H.C., Rahnama A., Sanchez O.M., Liu D., Gauthier T.J, & Melvin A.T. (2022). How Cargo Identity Alters the Uptake of Cell-Penetrating Peptide (CPP)/Cargo Complexes: A Study on the Effect of Net Cargo Charge and Length. Cells, 11(7), 1195.
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5

Solid-Phase Peptide Synthesis Protocol

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Fmoc-protected amino acids, piperidine, trifluoroacetic acid (TFA), 2-chlorotrityl chloride resin, 2 - (6 - chloro - 1H - benzotriazole - 1 - yl) - 1,1,3,3 - tetramethylaminium hexafluorophosphate (HCTU), and 1, 1-[bis(dimethylamino) methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxide hexafluorophosphate (HATU) were purchased from Advanced ChemTech (Louisville, KY). Diisopropylethylamine (DIEA), triisopropylsilane (TIPS), and N-methylmorpholine (NMM) were purchased from Sigma Aldrich (St. Louis, MO). Dimethylformamide (DMF) was purchased from Protein Technologies (Tucson, AZ). Dichloromethane (DCM), methanol (MeOH), and acetonitrile (CH3CN) were purchased from VWR International, LLC (Radnor, PA).
Parajuli P., Sable R., Shrestha L., Dahal A., Gauthier T., Taneja V, & Jois S. (2020). Modulation of costimulatory signal from CD2-CD58 proteins by a grafted peptide. Chemical biology & drug design, 97(3), 607-627.
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