Phenazine methosulfate fluoride pmsf

Dexamethasone (Dex), aluminum chloride, folin–ciocalteau reagent, 2,2-diphenyl-1-picrylhydrazyl (DPPH), sodium nitroprusside, Griess reagent (sulphanilamide and N-(1-Naphthyl) ethylenediamine dihydrochloride), tris-HCL, pyrogallol, thiobarbituric acid, trichloroacetic acid, 5,5-dithio-bis-2-nitrobenzoic acid (DTNB), cumene H₂O₂, reduced glutathione (GSH), and phenazine methosulfate fluoride (PMSF) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The total protein assay kit was purchased from BioMED (Cairo, Egypt). Total RNA extraction kit, cDNA synthesis kit, and SYBR green with low ROX were purchased from Enzynomics (Daejeon, Republic of Korea). Primers and antibodies were obtained from Willowfort (Nottingham, UK) and Cell Signaling Technology (Danvers, Massachusetts, USA), respectively. Other chemicals were of analytical grades.

Glycine, phenazine methosulfate fluoride (PMSF), Bradford reagent, and Tris-HCl were purchased from Sigma (Sigma Chemical Co., USA). Carbopol 934, propylene glycol, gentamicin sulfate, l-hydroxyproline, and p-dimethyl-amino-benzaldehyde were obtained from Himedia (Himedia Pvt. Labs., India). bFGF, COL3A1, Smad-2, Smad-3, Smad-4, and Smad-7, and β-actin primary and secondary antibodies and BCIP- (5-bromo-4-chloro-3′-indolyphosphate p-toluidine salt-) NBT reagents were procured from Santa Cruz (Santa Cruz, USA). PVDF membrane was from Millipore (Millipore Corp., USA) and Western Max-HRP-Chromogenic detection kit was purchased from Amresco (Amresco, USA). All other chemicals and reagents not mentioned were of the analytical grade.