Av 400 nmr spectrometer

All reagents were purchased from commercial sources and used without further purification unless otherwise stated. All experimental water used was deionized water.
¹H and ¹³C NMR spectra were recorded on a Bruker AV 400 NMR spectrometer (Bruker, Switzerland) by using deuterated chloroform as solvent and tetramethylsilane as internal standard. High resolution mass spectrometry (HRMS) were obtained on a LCMS-IT-TOF high resolution mass spectrometer (Shimadzu, Japan). Fluorescence spectra were recorded on a HORIBA Fluorolog-3 high-sensitivity fluorescence spectrometer (HORIBA, Japan). Single crystal X-ray diffraction data were performed on a Bruker APEX-II CCD diffractometer. During the data collection process, the crystals was kept at 150 K.

4-Bromo-1,8-naphthalic, morpholine, 2-methoxyethanol, ethylenediamine, chloroacetyl chloride, trimethylamine, hydrazine monohydrate, benzotriazol-1-yloxytris(dimethylamino)phosphonium hexafluorophosphate, and anhydride were bought from Innochem (Beijing, China). The other reagents were purchased from J&K Chemicals. All reagents were analytical level and employed without further purification. Nitrate salts of metal ions (Cd²⁺, Ni²⁺, Co²⁺, Ba²⁺, Ca²⁺, Pb²⁺, Fe³⁺, Cu²⁺, Mg²⁺, Mn²⁺, Zn²⁺, K⁺, Sr²⁺, Cr³⁺, Al³⁺, Ag⁺) and the perchlorate salt of Hg²⁺ were used for appraising the combining capacity of ions to compounds. Deionized water was applied to the entire experiment.
¹H and ¹³C NMR spectra were obtained on a Bruker AV400 NMR spectrometer using CD₂Cl₂ as the solvent and tetramethylsilane (TMS) as an internal reference. Mass spectrometry was conducted on an Agilent 1100 ion trap MSD spectrometer. HPLC spectra were taken on an Agilent 1260. An Agilent UV-8454 spectrophotometer was applied to the absorption measurements. The fluorescence spectra were collected using a Hitachi F-4500 fluorescence spectrophotometer. The melting points were determined by a WRS-1B melting point apparatus. The staining of cells was performed using an OLYMPUS FV1000 confocal laser scanning microscope. The fluorescence quantum yield value was recorded by a machine named QY C11347-11.

NMR spectra were obtained using a Bruker AV400 NMR spectrometer. Atomic force microscopy (AFM, Shimadzu SPM-9700) and optical microscopy (Dino-lite microscope) measurements were also performed. Use an electronic digital caliper to obtain the length and thickness of coating. Conduct rheological measurements on an MCR-302 rheometer (Anton Paar). The Scanning electron microscopy (SEM) measurements, confocal luminescence images, storage modulus (G′) and loss modulus (G′) was determined as described previously [17 (link)].