Mcherry l 7203

Manufactured by TriLink

MCherry (L-7203) is a fluorescent protein derived from the mCherry variant of the red fluorescent protein. It exhibits bright red fluorescence with excitation and emission maxima at 587 nm and 610 nm, respectively. MCherry can be used as a genetically encoded fluorescent marker in a variety of biological applications.

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Lab products found in correlation

Pierce d luciferin, by Thermo Fisher Scientific (2 mentions) 1 2 dioleoyl sn glycero 3 ethylphosphocholine, by Avanti Polar Lipids (1 mentions) 1 2 distearoyl sn glycero 3 phosphocholine dspc, by Avanti Polar Lipids (1 mentions) 1 2 distearoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) Cholesterol, by MP Biomedicals (1 mentions)

2 protocols using mcherry l 7203

Lipid-based Formulations for mRNA Delivery

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Dlin-MC3-DMA (MC3) and Dlin-KC2-DMA (KC2) were custom synthesized. 1,2-dioleyloxy-3-dimethylaminopropane (DODMA), 1,2-dioleoyl-3-trimethylammonium-propane (chloride salt) (DOTAP), N-(4-carboxybenzyl)-N,N-dimethyl-2,3-bis(oleoyloxy)propan-1-aminium (DOBAQ), 1,2-di-O-octadecenyl-3-trimethylammonium propane (chloride salt) (DOTMA), Dimethyldioctadecylammonium (Bromide Salt) (DDAB), 1,2-distearoyl-sn-glycero-3-ethylphosphocholine (chloride salt) (18:0 EPC), 1,2-dioleoyl-sn-glycero-3-ethylphosphocholine (chloride salt) (18:1 EPC), 1,2-distearoyl-3-dimethylammonium-propane (18:0 DAP), 1,2-stearoyl-3-trimethylammonium-propane (chloride salt) (18:0 TAP), and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC)were acquired from Avanti Polar Lipids. Cholesterol and 1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (DMG-PEG2k) was purchased from MP Biomedicals and NOF America Corporation, respectively. Pierce D-Luciferin was purchased from Thermo Fisher Scientific. Firefly luciferase (L-7202), EGFP (L-7201), and mCherry (L-7203) mRNA were obtained from Trilink Biotechnologies.

Patel S., Ryals R.C., Weller K.K., Pennesi M.E, & Sahay G. (2019). Lipid nanoparticles for delivery of messenger RNA to the back of the eye. Journal of controlled release : official journal of the Controlled Release Society, 303, 91-100.

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Formulating Lipid-Based Nanoparticles

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Dlin-MC3-DMA (MC3) was purchased through BioFine International Inc. 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) was acquired from Avanti Polar Lipids. Cholesterol and 1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (DMG-PEG2k) was purchased from MP Biomedicals and NOF America Corporation, respectively. Pierce D-Luciferin was purchased from Thermo Fisher Scientific. Firefly luciferase (L-7202), Cre (L-7211) and mCherry (L-7203) mRNA were all purchased from Trilink Biotechnologies.

Ryals R.C., Patel S., Acosta C., McKinney M., Pennesi M.E, & Sahay G. (2020). The effects of PEGylation on LNP based mRNA delivery to the eye. PLoS ONE, 15(10), e0241006.

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