The human U2OS cell line was grown in McCoy's 5A with L-glutamine medium (Cellgro, Corning) supplemented with 10% FBS (GenDEPOT) and 1% penicillin/streptomycin (Gibco). The 293T cell line was grown in DMEM (Cellgro, Corning) with 4.5 g/L glucose, L-glutamine, and sodium pyruvate medium supplemented with 10% FBS and 1% penicillin/streptomycin. Antibodies used are: Cezanne (Santa Cruz, sc-514402), RAP80 (Bethyl Laboratories, A300-763A), Abraxas (homemade), HA (Cell Signaling Technology, 3724s, 2367s), GFP (Invitrogen, A11122, A11120), K63 (EMD Millipore, 05-1308), ubiquitin (Santa Cruz, sc-8017), Ubc13 (Zymed, 37-1100), Ube2S (Cell Signaling Technology, 11878s), Lamin A (Sigma, L1293), GAPDH (Invitrogen, MA5-15738), BRCA1 (Santa Cruz, sc-6954), 53BP1 (Upstate, 05-726), γH2AX (Upstate, 05-636 JBW103), Rad18 (Abcam, ab188235), and pRPA32 (Bethyl, A300-245).
Ube2s
Manufactured by Cell Signaling Technology
Sourced in United States
Ube2S is an E2 ubiquitin-conjugating enzyme that functions in the ubiquitination pathway. It catalyzes the covalent attachment of ubiquitin to target proteins, which can lead to their degradation or alter their function.
Automatically generated - may contain errors
Lab products found in correlation
Brca1, by Santa Cruz Biotechnology (2 mentions)
Ubc13, by Zymo Research (2 mentions)
Ubiquitin, by Santa Cruz Biotechnology (1 mentions)
Mccoy s 5a, by Corning (1 mentions)
Rap80, by Fortis Life Sciences (1 mentions)
Lamin a, by Merck Group (1 mentions)
Rad18, by Abcam (1 mentions)
Prpa32, by Fortis Life Sciences (1 mentions)
Gapdh, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Corning (1 mentions)
Penicillin streptomycin, by Corning (1 mentions)
Ube2c, by Cell Signaling Technology (1 mentions)
Ecl western blotting detection system, by PerkinElmer (1 mentions)
Halt protease inhibitor cocktail, by Thermo Fisher Scientific (1 mentions)
Nitrocellulose membrane, by Bio-Rad (1 mentions)
Cyclin b1, by Santa Cruz Biotechnology (1 mentions)
Proliferating cell nuclear antigen (pcna), by Santa Cruz Biotechnology (1 mentions)
Flag tag (flag), by Merck Group (1 mentions)
γ tubulin, by Merck Group (1 mentions)
3 protocols using ube2s
1
Cell Culture and Antibody Usage
2
Protein Expression Analysis of MCF10A
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MCF10A protein was extracted using M-PER containing an EDTA-free Halt Protease Inhibitor Cocktail (Thermo Fisher Scientific, Massachusetts, USA), while proteins from all other cell lines were extracted using RIPA lysis buffer. Protein transfer was performed on a nitrocellulose membrane (Bio-Rad Laboratories, California, USA). Primary antibodies include: UBE2S (11878; Cell Signaling Technology, Massachusetts, USA; 1:1,000), UBE2C (14234; Cell Signaling Technology; 1:1,000), Numb (sc-136554; Santa Cruz Biotechnology, Texas, USA; 1:200), and ACTIN (3700; Cell Signaling Technology; 1:1,000). Protein was visualized with the ECL Western Blotting Detection System (PerkinElmer, USA), and a densitometry value was determined in terms of pixel intensity by ImageJ.
3
Antibodies Used in DNA Damage Response Study
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