Bx 795 s1274

Human CCA cell lines were HuCCT1 (JCRB, Osaka, Japan), HCCC9810, and RBE (Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China). HIBEpiC (ScienCell, Carlsbad, CA, USA) was normal human intrahepatic biliary epithelial cell line. The cells were cultured in RPMI‐1640 medium (Invitrogen, Waltham, MA, USA) containing 10%‐20% fetal bovine serum (Biological Industries, Cromwell, CT, USA), penicillin (Invitrogen) (100 U/mL), and streptomycin (Invitrogen) (100 U/mL). BX‐795 (S1274) and CPI‐613 (S1106) were purchased from Selleck Chemicals (Houston, TX, USA). N‐acetyl‐L‐cysteine (NAC, A7250) and Honokiol (H4914) were commercially purchased from Sigma‐Aldrich (St. Louis, MO, USA).

The mouse anti-Map-LC3 antibody (sc-376404), the rabbit polyclonal anti-mouse AIM2 antibody (sc-137967) and the goat polyclonal antibody TMEM173 (M-12) (sc-241049) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The rabbit anti-mouse β-actin (AP0060) was obtained from Bioworld Technology (Nanjing, Jiangsu, China). The rabbit Anti-NAK/TBK-1 antibody (ab40676) and rabbit anti-NAK/TBK-1 (phosphor S172) antibody (ab109272) were obtained from Abcam (Cambridge, UK). The rabbit TMEM173 polyclonal antibody (19851-1-AP) and rabbit IRF3 polyclonal antibody (11312-1-AP) are from Protein Tech (Wuhan, Hubei, China). The phosphor-IRF3 (Ser396) (4D4G) and the Acetylated-Lysine Antibody (9441) were from Cell Signaling Technology (Boston, Mass, USA). The goat anti-rabbit secondary antibody, rabbit anti-mouse secondary antibody and donkey anti-goat secondary antibody were obtained from Santa Cruz Biotechnology, Beijing ZSGB Biotechnology and Beijing Cowin Biotechnology (Beijing, China), respectively. The BX-795 (S1274) was from Selleckchem. The Fast Protein Precipitation and Concentration Kit were purchased from Beyotime Institute of Biotechnology (Shanghai, China). Reagents and apparatus used in immunoblotting assays were obtained from Bio-Rad (Hercules, CA, USA). The mouse interferon-β ELISA kit (CSB-E04945m) was from Cusabio (Wuhan, Hubei, China).