Animals were anesthetized with ketamine (87.5 mg/kg, i.p.), and xylazine (5 mg/kg, i.p.) administered in a volume of 1 mL/kg. Catheters (SILASTIC tubing, ID 0.51 mm, OD 0.94 mm, Dow Corning, Midland, MI) were implanted and secured into the jugular vein with suture thread (Surgical Specialties Corp., Wyomissing, PA). The catheter tubing was passed subcutaneously and exited from the back where it was connected to a cannula (Plastics One, Roanoke, VA, USA) embedded in a rubber harness (Instech, Plymouth Meeting, PA, USA). This harness was worn by the rat for the duration of the experiment. Carprofen (5 mg/kg, subcutaneous) was administered on the day of surgery and for the three following days for analgesia. Heparin (100 units/mL in 0.1 mL) was administered for the duration of the experiment. Catheter patency was tested periodically with methohexital sodium (10 mg/mL; Eli Lilly, Indianapolis, IN, USA).
Rubber harness
Manufactured by Instech
Sourced in United States
The Rubber Harness is a versatile laboratory equipment designed to provide a secure and reliable connection between various components. It serves as a durable and flexible interface, enabling the integration of different equipment and samples within a controlled environment. The core function of the Rubber Harness is to establish a stable and reliable physical connection, facilitating the seamless transfer of materials, liquids, or signals as required by laboratory protocols.
Automatically generated - may contain errors
9 protocols using rubber harness
1
Jugular Vein Catheterization in Rats
2
Jugular Catheter Implantation in Rats
3
Rat Jugular Vein Catheterization for Self-Administration
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Rats were anesthetized using ketamine (87.5 mg/kg, IP) and xylazine (5 mg/kg, IP). Ketorolac (2 mg/kg, IP) was administered post-operatively and 3 days following surgery for analgesia. Catheters (SILASTIC silicon tubing, ID 0.51 mm, OD 0.94 mm, Dow Corning, Midland, MI) were implanted in the jugular vein, secured with sutures, and passed subcutaneously between the shoulder blades to exit through the skin on the back. Catheter tubing was connected to a stainless-steel cannula (Plastics One, Roanoke, VA, USA) embedded in a rubber harness (Instech, Plymouth Meeting, PA, USA) that was worn for the duration of self-administration. The antibiotic cefazolin (100 mg/kg) was administered IV (0.1 mL) for 3 days post-surgery. Catheters were flushed with heparin (100 IU/mL; 0.1 mL) before and after each self-administration. Catheter patency was tested periodically with methohexital sodium (10 mg/mL; Eli Lilly, Indianapolis, IN, USA), which results in a temporary loss of muscle tone.
4
Jugular Vein Catheterization and Stereotaxic Surgery in Rats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Rats were anesthetized with ketamine (87.5 mg/kg, IP) and xylazine (5 mg/kg, IP). A catheter made from SILASTIC silicon tubing (ID 0.51 mm, OD 0.94 mm, Dow Corning, Midland, MI, United States) was implanted in the jugular vein and secured with suture thread. The catheter exited an incision on the back where it attached to a stainless-steel cannula inside a rubber harness (Instech, Plymouth Meeting, PA, United States). Rats were next placed into a stereotaxic frame (Stoelting, Wood Dale, IL, United States). A unilateral 22 gauge stainless guide cannula (Synaptech, Marquette, MI, United States) was implanted 2 mm above the NA core according to the coordinates: AP + 1.2 mm, ML ± 1.6 mm, DV −5.5 mm (Paxinos and Watson, 2006 ). Rats were administered the analgesic ketorolac (2 mg/kg, i.p.) on the day of surgery and for 3 days post-surgery. Catheter patency was maintained with heparinized saline (100 U/mL) daily and periodically verified with methohexital sodium (10 mg/mL i.v.; Eli Lilly, Indianapolis, IN, United States).
5
Intravenous Drug Self-Administration in Rats
6
Jugular Vein Catheterization in Rats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Rats were anesthetized using ketamine (87.5 mg/kg, IP) and xylazine (5 mg/kg, IP). Ketorolac (2 mg/kg, IP) was administered post-operatively and 3 days following surgery for analgesia. Catheters (SILASTIC silicon tubing, ID 0.51 mm, OD 0.94 mm, Dow Corning, Midland, MI) were implanted in the jugular vein, secured with sutures, and passed subcutaneously between the shoulder blades to exit through the skin on the back. Catheter tubing was connected to a stainless-steel cannula (Plastics One, Roanoke, VA, USA) embedded in a rubber harness (Instech, Plymouth Meeting, PA, USA) that was worn for the duration of self-administration. The antibiotic cefazolin (100 mg/kg) was administered IV (0.1 mL) for 3 days post-surgery. Catheters were flushed with heparin (100 IU/mL; 0.1 mL) before and after each self-administration. Catheter patency was tested periodically with methohexital sodium (10 mg/mL; Eli Lilly, Indianapolis, IN, USA), which results in a temporary loss of muscle tone.
7
Surgical Procedures for Rodent Self-Administration Studies
8
Surgical Implantation of Intravenous Catheters and Stereotactic Cannulae for Rodent Self-Administration Studies
9
Surgical Procedures for Rat Addiction Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For both experiments, rats were anesthetized with ketamine (males 87.5 mg/kg, IP; females 60 mg/kg, IP) and xylazine (males and females 5 mg/kg, IP) prior to surgery. Carprofen (1 mg/kg, SC) was administered as an analgesic for three days following surgery. Catheters (SILASTIC silicon tubing, ID 0.51 mm, OD 0.94 mm, Dow Corning, Midland, MI) were implanted in the jugular vein and secured via suture thread. Catheters passed subcutaneously through the shoulder region and exited the back of the animal. The catheter was secured to a cannula (Plastics One, Roanoke, VA, USA) embedded in a rubber harness (Instech, Plymouth, Meeting, PA, USA). This harness was worn for the duration of the self-administration portion of the study. Catheters were flushed daily with 0.2 mL of heparinized saline (100 IU/mL) and catheter patency was verified periodically via intravenous administration of methohexital sodium (10 mg/mL, IV), which produces a temporary loss of muscle tone.
Rats used for Experiment 2 also underwent stereotaxic implantation of bilateral guide cannulas (Plastics One, Roanoke, VA, USA) 2 mm above the NA core (AP 1.2 mm, ML 2.5 mm, DV -5.5 mm). Cannulas were secured using dental cement and stainless-steel skull screws, and a stylet was inserted into the cannula until microinjections were administered prior to the reinstatement tests.
Rats used for Experiment 2 also underwent stereotaxic implantation of bilateral guide cannulas (Plastics One, Roanoke, VA, USA) 2 mm above the NA core (AP 1.2 mm, ML 2.5 mm, DV -5.5 mm). Cannulas were secured using dental cement and stainless-steel skull screws, and a stylet was inserted into the cannula until microinjections were administered prior to the reinstatement tests.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!