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Cocaine hydrochloride c5776

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Cocaine hydrochloride (C5776) is a chemical compound commonly used in research and laboratory settings. It is a white crystalline powder that is the hydrochloride salt of the natural alkaloid cocaine. This product is intended for use in controlled research environments and should be handled with appropriate safety precautions.

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Lab products found in correlation

Caspase 1 p20, by Santa Cruz Biotechnology (1 mentions) Cd11b, by Novus Biologicals (1 mentions) Goat anti mouse hrp, by Santa Cruz Biotechnology (1 mentions) Goat anti rabbit hrp, by Santa Cruz Biotechnology (1 mentions) Il 1 beta, by Abcam (1 mentions) Mcc950 cp 456773, by Merck Group (1 mentions) Lps l2880, by Merck Group (1 mentions) Atp tlrl atpl, by InvivoGen (1 mentions) Actin, by Merck Group (1 mentions) C57bl 6j wt mice, by Jackson ImmunoResearch (1 mentions) Goat anti rabbit sc 2004, by Santa Cruz Biotechnology (1 mentions)

3 protocols using cocaine hydrochloride c5776

1

NLRP3 Inflammasome Activation Assay

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Antibodies and reagents used in this work were purchased from the indicated sources: NLRP3 (AdipoGen; AG-20B-0014); ASC (Novus; NB1–78978 for western blots and Adipogen; AG-25B-0006 for IHC), caspase-1 p20 (Santa Cruz; sc-398715), Iba-1 (Novus NB1001028 or Wako; 19–19741), CD11b (Novus; NB11089474), goat anti-mouse-HRP (Santa Cruz Biotechnology; sc-2005) and goat anti-rabbit-HRP (Santa Cruz Biotechnology; sc-2004); IL-1 beta (Abcam; ab9722); actin (Sigma-Aldrich; A1978). Cryopyrin/NLRP3 siRNA (sc-45470) was from Santa Cruz Biotechnology. MCC950/CP-456773 (Sigma; pz0280) was from Sigma-Aldrich. Cocaine hydrochloride (C5776) and LPS (L2880) was from Sigma-Aldrich. Sterile water was used to dissolve cocaine and LPS. ATP (tlrl-atpl) was from Invivogen. FAM-FLICA caspase assay kit (#97) was from ImmunoChemistry
Chivero E.T., Thangaraj A., Tripathi A., Periyasamy P., Guo M.L, & Buch S. (2021). NLRP3 inflammasome blockade reduces cocaine-induced microglial activation and neuroinflammation. Molecular neurobiology, 58(5), 2215-2230.
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2

Cocaine Addiction Study in Mice

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CX3CR1-GFP (002582) and WT C57BL/6J mice were purchased from Jackson lab (Bar Harbor, ME, USA) and kept in the lab. All the animals were housed under conditions of constant temperature and humidity on a 12 h light, 12 h dark cycle, with lights on at 07:00 h. Food and water were available ad libitum. All animal procedures were performed according to the protocols approved by the Institutional Animal Care and Use Committee of the University of Nebraska Medical Center (project IACUC # 18-030-04-FC, and the most recent three-year renewal approval date 8 March 2021) and the National Institutes of Health. Cocaine hydrochloride (C5776) was purchased from Sigma and was freshly dissolved in 0.9% saline.
Guo M.L., Chivero E.T., Callen S.E, & Buch S. (2021). NLRP3 Inflammasome Is Involved in Cocaine-Mediated Potentiation on Behavioral Changes in CX3CR1-Deficient Mice. Journal of Personalized Medicine, 11(10), 963.
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3

Molecular Mechanisms of miR-124 Regulation

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Cocaine hydrochloride (C5776), and 5-Azacytidine (5-AZA; A3656) were purchased from Sigma-Aldrich. TaqMan® microRNA assays for miR-124 (001182) quantification was purchased from Applied Biosystems; miR-124 mimic and inhibitor and negative control were purchased from Dharmacon; Lentivirus-miR-124 and control lentivirus were purchased from Biosettia (miR-LV084 and LV000). Antibodies to TLR4 (sc-10741), IRAK1 (sc-7883), TRAF6 (sc-7221), MyD88 (sc-11356) and KLF4 (sc-20691) were purchased from Santa Cruz Biotechnology. Goat anti-rabbit (sc-2004) and goat anti-mouse (sc-2005) secondary antibodies were also from Santa Cruz Biotechnology. Realtime qPCR primers for TNFα, IL6, IL1β, CCL2, TGFβ, IL4, IL10 and Arg1 were commercially available from Invitrogen.
Periyasamy P., Liao K., Kook Y.H., Niu F., Callen S.E., Guo M.L, & Buch S. (2017). Cocaine-mediated downregulation of miR-124 activates microglia by targeting KLF4 and TLR4 signaling. Molecular neurobiology, 55(4), 3196-3210.
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