Animal studies were performed according to protocols reviewed and approved by the Institutional Animal Care and Use Committee of University of Florida and University of Texas Health Science Center at San Antonio. Female nude mice at 4–6-weeks old were purchased from the Jackson Laboratory. 5×105 ES-2-BLI cell were intravenously injected into the mice at Day 0. For the SRMS knockdown experiment, vehicle control or cisplatin was given starting from Day 3 at 10 mg/kg at the first week. For the pLX4720 efficacy experiment, vehicle control or pLX4720 was started on Day 3 at 25 mg/kg, daily; cisplatin administration was started on Day 7 at 2.5 mg/kg per week for ES-2 parental cells and 5mg/kg for cisplatin resistant cells. Tumor growth was monitored by bioluminescence imaging (BLI) as previously described(51 (link), 55 (link)).
Female nude mice
Manufactured by Jackson ImmunoResearch
Sourced in United States, Montenegro
Female nude mice are a type of laboratory mouse that lacks a functional immune system. They are commonly used in biomedical research to study various diseases, test new treatments, and evaluate the efficacy of drugs and therapies.
Automatically generated - may contain errors
Lab products found in correlation
Matrigel, by Thermo Fisher Scientific (2 mentions)
Nci n87, by American Type Culture Collection (2 mentions)
Mycoalert testing kit, by Lonza (2 mentions)
Dmem medium, by Jackson ImmunoResearch (2 mentions)
Female nu nu nude mice, by Charles River Laboratories (1 mentions)
Prolab isopro rmh 3000, by LabDiet (1 mentions)
Ivis lumina 3 in vivo imaging system, by PerkinElmer (1 mentions)
Matrigel, by BD (1 mentions)
Nude mice, by Jackson ImmunoResearch (1 mentions)
C57bl 6 mice, by Taconic Biosciences (1 mentions)
Hbss matrigel, by Corning (1 mentions)
Female c57bl 6 mice, by Jackson ImmunoResearch (1 mentions)
Balb c, by Jackson ImmunoResearch (1 mentions)
31 protocols using female nude mice
1
Evaluating Ovarian Cancer Therapies in Nude Mice
2
Xenograft Mouse Model of AKT1 Signaling
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DLD-1-AKT1/2−/− (2 × 106) cells stably expressing AKT1-WT or AKT1-R391K mutant or DLD-1 cells depleted PRMT5 and/or overexpressing myr-AKT1 were injected into the flank of 5-week-old female nude mice (The Jackson Laboratory). Tumor size was measured every 2 days with an electronic caliper. The tumor volume was calculated using the following formula: L × W2 × 0.5, where L is the longest diameter and W is the shortest diameter. After 19–21 days, mice were killed. The solid tumors were dissected and weighed. All mice are housed in 22 °C, 50–60%, humidity and a 12 h-light/12 h-dark cycle. All mouse experiments were conducted under Protocol #IACUC-2018-00604 approved by the MUSC Institutional Animal Care and Use Committee.
3
Murine Xenograft Tumor Model
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Female nude mice were obtained from Jackson Laboratory. Experimental protocols were approved by the Institutional Animal Care and Use Committee at Wake Forest health Science. Female NU/NU Nude Mice from Charles River Laboratories were used for animal experiments. Mice were housed in a 12h light/12 h dark cycle with temperature-controlled room. The animal rooms are provided with 100% fresh, HEPA filtered air at 10-15 air changes per hour. Room temperatures are controlled by reheat units within each room, and are maintained within the range of 70°F ± 2° F. The humidity levels are controlled globally, and it is maintained between 30-70%. The mice were fed with a standard chow (Prolab Isopro RMH 3000, 5P00, LabDiet) and water ad libitum. Approximately 1 million cells were subcutaneously injected into the mammary fat pad of 7- to 9-week-old mice. Tumor length (L) and width (W) were measured by caliper, and tumor size was calculated using the formula, 1/2* LW2 (link). The maximum tumor size is 2cm of the length and maximal tumour size/burden was not exceeded in this study. Tumor growth were also monitored by bioluminescence imaging using the IVIS lumina III in vivo imaging system (Perkin Elmer). Living Image (Caliper Life Science) version 4.7.3 was used to analyze the bioluminescence level. Tumor wet weight were measured at the endpoint.
4
Nude Mice Anesthesia Protocol
5
NCI-N87 Cell Line Maintenance and Xenograft Model
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The NCI-N87 cell line was purchased from ATCC and maintained according to ATCC recommendations. Cells were stored at 37°C in 5% CO2 and passaged 2 to 3 times per week up to passage 50 using RPMI1640 (NCI-N87) each supplemented with 10% (v/v) FBS, 50 U/mL penicillin and 50 μg/mL streptomycin. Mycoplasma tests were performed annually using Mycoalert Testing Kit (Lonza). All animal studies were performed in accordance with and approval from the University of Michigan Institutional Animal Care and Use Committee and Association for Assessment and Accreditation of Laboratory Animal Care International guidelines in 6- to 8-week-old female nude mice (Jackson Labs). Briefly, 5 million NCI-N87 cells were injected subcutaneously into the left flank in 50% v/v Matrigel (Fisher Scientific) and treated as described below.
6
Evaluating GO-203 in a Murine Tumor Model
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Six-week-old female nude mice (The Jackson Laboratory) were injected subcutaneously into the flank with 5×106 tumor cells in 100 μL of a 1:1 solution of medium and Matrigel (BD Biosciences). When the mean tumor volume reached 100–150 mm3, the mice were pair-matched into groups and treated intraperitoneally with PBS or GO-203 (12 μg/g body weight) daily. Tumor measurements and body weights were recorded twice per week. These studies were conducted in accordance with the ethical regulations required for approval by the Dana-Farber Cancer Institute Animal Care and Use Committee (IACUC) under protocol 03–029.
7
Xenograft Mouse Tumor Study Protocol
8
Combination Targeted Therapy for TNBC
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All in vivo studies were approved by, and conducted in accordance with the guidelines of the IACUC at Houston Methodist Research Institute. MDA-MB-231 cells (5 million cells per mouse) were injected into the mammary fat pad of female nude mice (The Jackson Laboratory, Bar Harbor, ME). Tumor growth was monitored by external caliper measurement. General condition of the mice was monitored daily. Treatment was initiated when mean tumor volume was approximately 200 mm3 for all groups. Mice (n=10 per cohort) were treated with ABT-888 (25 mg/kg, diluted in sterile, acidified normal saline (pH 4.0) P.O. daily, 5 days per week), vorinostat (30 mg/kg diluted in DMSO, i.p. daily, 5 days per week) or ABT-888 and vorinostat for 3 weeks. Mice were humanely euthanized when tumor volumes exceeded 1500 mm3. Survival of the mice is reported by a Kaplan Meier plot [27 (link), 33 ]. For biomarker analysis, a cohort of mice was treated with ABT-888 and/or VS for 1 week and then humanely euthanized. Tumors were excised and cell lysates were prepared for immunoblot analysis.
9
Nude Mice Xenograft Tumor Model
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Female nude mice were purchased from the Jackson lab and randomly assigned into 3 groups (3 mice/group). All animal experiments were conducted in accordance with NIH regulations and approved by the Institutional Animal Care and Use Committee of the University of South Carolina (Protocol No. 100851, approval date: 23 September 2015). Total 5 million HCT 116 cells suspended in PBS were subcutaneously injected into mice. When the tumor volume reached about 30 mm3, the mice were anesthetized with 3% of isoflurane and treated with LBA melittin nanoparticle at 2 mg/kg via intratumoral injection. For control groups, the same amount of buffer used in preparing complex or non-targeted nanoparticles was employed.
10
Radioresistant Tumor Inoculation and Treatment
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For the subcutaneous tumor inoculation, 5 × 106 wild-type U251 cells in 200 μl PBS were injected into 6 weeks old female nude mice (The Jackson Laboratory, Stock No: 002019) in the right flanks (n = 7 per group). Tumor volumes were estimated and calculated using the formula for a spheroid: V = Length x width2/2. Tumors were protruded for fractionated irradiation (2 Gy/day) when tumor volumes reached approximately 200 mm3 (tumors received radiation at day 16, day 17, day 18, day 19 and day 20, respectively). Tumor volumes shrank but re-reached approximately 200 mm3 8 days after RT, and the regrowing tumors were used as radioresistant tumors. Cryptotanshinone (25 mg/kg, 20 mg/ml in 25 μl DMSO), selumetinib (25 mg/kg, 20 mg/ml in 25 μl DMSO) or cryptotanshinone combined with selumetinib was then intratumorally injected 4 h before tumors receiving RT (2 Gy/day for 2 days). The inhibitors were administered every other day till the end of experiment. Tumor volumes were estimated and calculated at the indicated times.
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