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On target sirna smartpools

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ON-TARGET siRNA SMARTpools are a collection of four individual siRNA duplexes designed to target a specific gene. The siRNA sequences are optimized for efficient knockdown of target gene expression.

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Lab products found in correlation

Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions) Succinylacetone, by Merck Group (2 mentions) Mannitol, by Merck Group (2 mentions) Isoproterenol, by Merck Group (2 mentions) 8 br camp, by Merck Group (2 mentions) Protoporphyrin 9, by Merck Group (2 mentions) Cl 316 243, by Cayman Chemical (2 mentions) Forskolin, by Chem-Impex (2 mentions) Insulin novolin, by Novo Nordisk (2 mentions) Gibco branded cell culture products, by Thermo Fisher Scientific (2 mentions) Hemin, by Merck Group (2 mentions) Hek293t cell, by American Type Culture Collection (2 mentions) Oligomycin a, by Merck Group (2 mentions) Antimycin a, by Merck Group (2 mentions) Norepinephrine, by Merck Group (2 mentions) Complete edta free protease inhibitor cocktail, by Roche (2 mentions) Human mdm2 sirna, by Horizon Discovery (1 mentions) Sigenome control pool, by Horizon Discovery (1 mentions) Neon transfection system, by Thermo Fisher Scientific (1 mentions) Nis elements software, by Nikon (1 mentions)

Spelling variants of this product

SMARTpool (292 citations) SMARTpool siRNA (284 citations) SiRNAs (161 citations) ON-TARGET (46 citations) ON-TARGET SMARTpool (12 citations) ON-TARGET SMARTpool siRNA (11 citations) ON-TARGET plus SMARTpools siRNAs (2 citations)

3 protocols using on target sirna smartpools

1

Heme Regulation of Cellular Metabolism

Cited 1 time
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Hemin, protoporphyrin IX, oligomycin A, carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP), rotenone, antimycin A, 3-isobutyl-1-methylxanthine (IBMX), BSA, mannitol, norepinephrine, isoproterenol, 8-Br-cAMP, and succinylacetone were purchased from Sigma-Aldrich. CL 316,243 was obtained from Cayman Chemical. Forskolin was obtained from Chem Impex International. Insulin (Novolin) was purchased from Novo-Nordisk. Complete EDTA-free protease inhibitor cocktail was obtained from Roche. DMEM and other Gibco-branded cell culture products were purchased from Thermo Fisher. Heme-depleted FBS was prepared by treating FBS with 20 mM ascorbic acid for 16 hr, followed by 24 hr dialysis against PBS. Heme depletion was verified by measuring optical absorbance at 405 nm. CPAG-1 was synthesized as previously reported5 (link). ON-TARGET siRNA SMARTpools against human PGRMC2 (L-010639-00-0005), PGRMC1 (L-010642-00-0005), and mouse Nr1d1 (L-051721-00-0005), and BACH1 (L-042956-01-0005), as well as a Non-targeting Pool (D-001810-10-05) were purchased from Dharmacon. HEK293T cells were obtained from ATCC (CRL-3216) having undergone short-tandem repeat verification. Cells were routinely tested for mycoplasma and were never positive.
Galmozzi A., Kok B.P., Kim A.S., Montenegro-Burke J.R., Lee J.Y., Spreafico R., Mosure S., Albert V., Cintron-Colon R., Godio C., Webb W.R., Conti B., Solt L.A., Kojetin D., Parker C.G., Peluso J.J., Pru J.K., Siuzdak G., Cravatt B.F, & Saez E. (2019). PGRMC2 is an Intracellular Heme Chaperone Critical for Adipocyte Function. Nature, 576(7785), 138-142.
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2

Heme Regulation of Cellular Metabolism

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Hemin, protoporphyrin IX, oligomycin A, carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP), rotenone, antimycin A, 3-isobutyl-1-methylxanthine (IBMX), BSA, mannitol, norepinephrine, isoproterenol, 8-Br-cAMP, and succinylacetone were purchased from Sigma-Aldrich. CL 316,243 was obtained from Cayman Chemical. Forskolin was obtained from Chem Impex International. Insulin (Novolin) was purchased from Novo-Nordisk. Complete EDTA-free protease inhibitor cocktail was obtained from Roche. DMEM and other Gibco-branded cell culture products were purchased from Thermo Fisher. Heme-depleted FBS was prepared by treating FBS with 20 mM ascorbic acid for 16 hr, followed by 24 hr dialysis against PBS. Heme depletion was verified by measuring optical absorbance at 405 nm. CPAG-1 was synthesized as previously reported5 (link). ON-TARGET siRNA SMARTpools against human PGRMC2 (L-010639-00-0005), PGRMC1 (L-010642-00-0005), and mouse Nr1d1 (L-051721-00-0005), and BACH1 (L-042956-01-0005), as well as a Non-targeting Pool (D-001810-10-05) were purchased from Dharmacon. HEK293T cells were obtained from ATCC (CRL-3216) having undergone short-tandem repeat verification. Cells were routinely tested for mycoplasma and were never positive.
Galmozzi A., Kok B.P., Kim A.S., Montenegro-Burke J.R., Lee J.Y., Spreafico R., Mosure S., Albert V., Cintron-Colon R., Godio C., Webb W.R., Conti B., Solt L.A., Kojetin D., Parker C.G., Peluso J.J., Pru J.K., Siuzdak G., Cravatt B.F, & Saez E. (2019). PGRMC2 is an Intracellular Heme Chaperone Critical for Adipocyte Function. Nature, 576(7785), 138-142.
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3

Wound Healing Assay with siRNA Knockdown

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Cells (800,000/well) were plated in a six-well plate one night before the experiment. Scratches were created using a 200-μl pipette tip. Cells were then rinsed three times with fresh medium. Wound closure was observed within the scrape line and photographed by phase-contrast microscopy. Wound area was measured and quantified by using NIS-Elements software (Nikon Instruments, Melville, NY, USA). Thirty fields per condition were recorded, and three independent experiments were performed. Transient electroporation of small interfering RNA (siRNA) was carried out using an Invitrogen Neon transfection system (Life Technologies) with ON-TARGET siRNA smartpools obtained from Dharmacon (Lafayette, CO, USA): siGENOME™ Control Pool (catalogue no. D-001206-13-20), human mdm2 siRNA (catalogue no. L-003279-00), and human mdm4 siRNA (catalogue no. L-006536-02-0005).
Gao C., Xiao G., Piersigilli A., Gou J., Ogunwobi O, & Bargonetti J. (2019). Context-dependent roles of MDMX (MDM4) and MDM2 in breast cancer proliferation and circulating tumor cells. Breast Cancer Research : BCR, 21, 5.
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