Hplc grade acetonitrile mecn

Manufactured by Merck Group

Sourced in Germany

HPLC-grade acetonitrile (MeCN) is a highly pure organic solvent commonly used as a mobile phase component in high-performance liquid chromatography (HPLC) analyses. It has a low viscosity and UV transparency, making it suitable for HPLC applications.

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Lab products found in correlation

2 nbdg assay kits, by Cayman Chemical (2 mentions) Sephadex lh 20 gel, by GE Healthcare (2 mentions) Methanol, by Merck Group (2 mentions) Milli q system, by Merck Group (2 mentions) Mannitol, by Merck Group (1 mentions) Carboxy h2dcfda 5 and 6 carboxy 2 7 dichlorodihydrofluorescein diacetate, by Thermo Fisher Scientific (1 mentions) Streptozotocin (stz), by Merck Group (1 mentions) Milli q plus system, by Merck Group (1 mentions)

6 protocols using hplc grade acetonitrile mecn

HPLC-based Metabolite Analysis

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High performance liquid chromatography (HPLC)-grade solvents were used for chromatography and all the other chemicals were of analytical-reagent grade. HPLC-grade acetonitrile (MeCN) and methanol were purchased from Merck Chemical Company (Darmstadt, Germany). Sephadex LH-20 gel was obtained from GE Health Care (Uppsala, Sweden). The 2-NBDG assay kits were purchased from Cayman Chemical (Ann Arbor, MI, USA). Minimum Essential Medium-α (MEM-α) and fetal bovine serum (FBS, and antibiotics (100 U/mL penicillin and 100 μg/mL streptomycin)) were obtained from Hyclone (Logan, UT, USA).

Liu C., Liu H., Wen Y., Huang H., Hao J., Lv Y., Qin R, & Yang X. (2019). Aspernolide A Inhibits the Proliferation of Human Laryngeal Carcinoma Cells through the Mitochondrial Apoptotic and STAT3 Signaling Pathways. Molecules, 24(6), 1074.

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HPLC Reagent Preparation Protocol

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HPLC-grade acetonitrile (MeCN) was purchased from E. Merck (Darmstadt, Germany) and ammonia (AR grade) was obtained from Beihua Fine Chemicals Co., Ltd. (Beijing, China). The water used for HPLC was purified by a Milli-Q system (Millipore, Milford, MA, U.S.A.).

Tan P., Chen X., He R, & Liu Y. (2014). Analysis of the metabolites of mesaconitine in rat blood using ultrafast liquid chromatography and electrospray ionization mass spectrometry. Pharmacognosy Magazine, 10(38), 101-105.

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Isolation and Characterization of Aconitum Alkaloids

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Streptozotocin and mannitol were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Carboxy-H₂DCFDA (5-(and-6)-carboxy-2′,7′-dichlorodihydrofluorescein diacetate) and DHE were purchased from Life Technologies Corporation (USA). 2-(6-Amino-3-imino- 3H-xanthen-9-yl) benzoic acid methyl ester, hydrochloride (Rh123) was purchased from Dojindo Laboratories (Japan). All antibodies used in this study were from Abcam (Abcam, CA, USA). Aconitine, mesaconitine and hyperaconitine were prepared by our laboratory. These compounds were isolated from the ethyl acetate extract of Fuzi lateral root. HPLC-grade acetonitrile (MeCN) were purchased from E. Merck (Darmstadt, Germany) and ammonia (AR grade) was obtained from Beihua Fine Chemicals Co., Ltd. (Beijing, China). The water used for HPLC was purified by a Milli-Q system (Millipore, Milford, MA, USA). Streptozotocin (STZ) was procured from Sigma-Aldrich, USA.

Han J., Tan P., Li Z., Wu Y., Li C., Wang Y., Wang B., Zhao S, & Liu Y. (2014). Fuzi Attenuates Diabetic Neuropathy in Rats and Protects Schwann Cells from Apoptosis Induced by High Glucose. PLoS ONE, 9(1), e86539.

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Isolation and Characterization of Bioactive Compounds

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HPLC grade acetonitrile (MeCN) and MeOH were purchased from Merck (Darmstadt, Germany). Ultrapure water was prepared by using a Millipore Milli-Q-Plus system (Bedford, MA, USA). (3R, 5R, 6S, 7S, 10R)-7-(2-hydroxy-2-propyl)-10-methyl-4-methyleneperhy, dronaphthal-ene-3, 5, 6-triol acetone solvate; (+)-edusmance-4, (14)-ene-11, 13-diol; linarin; luteolin; apigenin; tricin; 5, 3’,4’-trimethyl-6, 7-dimethoxy flavones; cumambrin A; acacetin; angeloylcumambrin B were prepared and purified by our lab. The purity was > 98%. The chemical structures of all reference substances are shown in Figure 2.

Fu L., Wang P., Sun Y., Wang Y., Zhao J., Ye Y., Zhang Y, & Bi Y. (2015). High performance liquid chromatography time of flight electrospray ionization mass spectrometry for quantification of sesquiterpenes in Chrysanthemi indici Flos active extract. Pharmacognosy Magazine, 11(44), 740-744.

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Pesticide Quantification via m-PFC and QuEChERS

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Analytical reference standards of the 350 pesticides were purchased from Alta Scientific Co., Ltd. Stock solutions (Tianjin, China) and were stored at −20 °C. Internal standards of heptachlor epoxide were purchased from Aldrich-Sigma (Shanghai, China).
HPLC grade acetonitrile (MeCN) was purchased from Merck (Darmstadt, Germany).
Two types of m-PFC were provided by China Agricultural University (Beijing, China). m-PFC cartridge (simple matrix): 15 mg MWCNTs + 15 mg PSA + 150 g MgSO₄; m-PFC (complex matrix): 25 mg MWCNTs + 15 mg PSA + 150 g MgSO₄. Two types of traditional QuEChERS purification package were purchased from Thermo Fisher Scientific (Beverly, MA, USA). QuEChERS (simple matrix): 50.0 mg PSA + 150.0 mg MgSO₄; QuEChERS (complex matrix): 50.0 mg PSA + 150.0 mg MgSO₄ + 50 mg C₁₈ + 50 mg GCB. Three kinds of salting out packages for the QuEChERS method: 4 g MgSO₄, 1 g NaCl; AOAC 2007.01 Method: 6 g MgSO₄, 1.5 g sodium acetate (NaOAc); EN15662 Method: 4 g MgSO₄, 1 g NaCl, 1 g trisodium citrate dihydrate (TSCD), 0.5 g disodium hydrogen sesquihydrate (DHS) respectively were provided by Lumiere Technologies (Beijing, China).

Meng Z., Li Q., Cong J., Huang Y., Wang D., Pan C., Fan S, & Zhang Y. (2021). Rapid Screening of 350 Pesticide Residues in Vegetable and Fruit Juices by Multi-Plug Filtration Cleanup Method Combined with Gas Chromatography-Electrostatic Field Orbitrap High Resolution Mass Spectrometry. Foods, 10(7), 1651.

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HPLC Solvent Optimization for Chromatography

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High performance liquid chromatography (HPLC) grade solvents used for chromatography and all the other chemicals were of analytical-reagent grade.
HPLC-grade acetonitrile (MeCN) and methanol were purchased from Merck Chemical Company (Darmstadt, Germany). Sephadex LH-20 gel was obtained from GE Health Care (Uppsala, Sweden). The 2-NBDG assay kits were purchased from Cayman Chemical (Ann Arbor, Michigan USA). Minimum Essential Medium-ɑ (MEM-ɑ) and fetal bovine serum (FBS, and antibiotics (100 U/mL penicillin and 100 μg/mL streptomycin)) were obtained from Hyclone (Logan, UT, USA).

Wen Y., Lv Y., Hao J., Chen H., Huang Y., Liu C., Huang H., Ma Y, & Yang X. (2020). Two new compounds of Penicillium polonicum, an endophytic fungus from Camptotheca acuminata Decne. Natural product research, 34(13).

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