Q150r sputter coater unit

The surface morphology/shape of pure APG, pure PL and various SDs prepared by different techniques were studied using “Scanning Electron Microscopy (SEM)”. The samples from each material were fixed on the stubs with the help of “Adhesive Carbon Tape (SPI Supplies, West Chester, PA, USA)” and coated with gold under vacuum in a “Q150R Sputter Coater Unit (Quorum Technologies Ltd., East Sussex, UK)” in an argon atmosphere at 20 mA for about 60 sec. The photomicrographs of each sample were recorded with the help of “SEM Microscope (Zeiss EVO LS10; Cambridge, UK)” (Alshehri et al., 2017a (link)).

Size and surface morphology of dry powder of 5-FU-PLGA NPs were examined under the scanning electron microscope SEM EVO LS10 (Carl-Zeiss, Cambridge, UK). Particles were mounted on double-sided adhesive carbon tape (SPI Supplies, West Chester, USA) and coated under high-vacuum evaporator with gold in a Q 150R sputter coater unit (Quorum Technologies Ltd., East Sussex, UK) in an argon atmosphere at 20 mA for 120 s. The coated samples were scanned, and photomicrographs were taken at an acceleration voltage of 1–10 kV.

The surface morphology of the liposomes was studied using scanning electron microscopy (Zeiss EVO LS10, Cambridge, UK).20 Samples were fixed on stubs using both side adhesive carbon tape (SPI Supplies, West Chester, PA, USA) and coated under vacuum with gold in a Q150R sputter coater unit obtained from Quorum Technologies Ltd. (East Sussex, UK) in an argon atmosphere at 20 mA for 120 seconds. After mounting, the samples were viewed and photographed. Particles size analyzer was used to determine the size of the liposomal entity (Mastersizer 2000, Malvern Instruments, Malvern, UK). Laser diffraction technique was applied to determine the liposomal size distribution by immersing liposomes in oil medium at a temperature of 25°C±1°C and scattering light at 90°.