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Ab193651

Manufactured by Abcam

Ab193651 is a lab equipment product manufactured by Abcam. It is designed for use in laboratory settings, but a detailed description of its core function cannot be provided while maintaining an unbiased and factual approach. Further information is not available.

Automatically generated - may contain errors

2 protocols using ab193651

1

Autophagy and Apoptosis Modulation

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Spermidine (S0266), 3‐methyladenine (M9281), TBHP (458139) and type II collagenases (1148090) were purchased from Sigma‐Aldrich (St Louis, MO, USA). p62 (ab56416), LC‐3(ab128025), Beclin‐1(ab6242), Atg7(ab52472), cleaved caspase‐3(ab32042), Bax(ab32503) and Bcl‐2(ab59348) antibodies were purchased from Abcam (Cambridge, UK). FITC‐labelled and horseradish peroxidase‐labelled secondary antibodies were purchased from Abcam (ab7086, ab191866, ab193651). 4′, 6‐diamidino‐2‐phenylindole (DAPI) was obtained from Beyotime (C1002, Shanghai, China).
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2

Histone Acetylation and Cell Cycle Regulation

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The primary antibodies that were used were rabbit anti‐histone H4 lysine 5 acetylation (#9627, CST), rabbit anti‐lysine acetylation (ab190479; Abcam), rabbit anti‐Histone H3 lysine 18 acetylation (AJ1357a; Abgent, USA), anti‐CyclinB1 (ab151269; Abcam), rabbit anti‐histone H4 lysine 9 acetylation (07‐352; Millipore), anti‐GAPDH (92590; Millipore, USA), anti‐CTGF (ab6992; Abcam, USA), mouse anti‐p21 (SC‐817; Santa Cruz), rabbit anti‐TXNIP (ab188865, Abcam,USA), goat anti‐gamma H2AX (Santa Cruz), mouse anti‐BST2 (ab88523; Abcam), rabbit anti‐Histone H3 lysine 4 trimethylation (07‐437; Millipore), rabbit anti‐Histone H3 trimethylation lysine 27 (07‐449; Millipore), rabbit anti‐Histone H3 trimethylation lysine 9 (07‐442; Millipore), and goat anti‐GFP (ab6673; Abcam). The secondary antibodies that were used were anti‐rabbit IgG‐HRP (ab191866 Abcam), anti‐mouse IgG‐HRP (ab193651 and ab193652; Abcam), donkey anti‐goat‐HRP (31400, Pierce), and DyLight 488 Goat Anti‐Rabbit (111‐055‐003, Jackson).
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