Twin trough chamber

Manufactured by CAMAG

Sourced in Switzerland

The Twin Trough Chamber is a laboratory equipment used for chromatographic separation. It consists of two parallel troughs that hold the solvents used in the chromatographic process. The device provides a contained and controlled environment for the development of thin-layer chromatography (TLC) plates.

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Lab products found in correlation

Tlc scanner 4, by CAMAG (5 mentions) Linomat 5, by CAMAG (4 mentions) Automatic tlc sampler 4, by CAMAG (4 mentions) Linomat 5 applicator, by CAMAG (4 mentions) Tlc visualizer, by CAMAG (3 mentions) Uv chamber, by CAMAG (3 mentions) Tlc scanner 3, by CAMAG (2 mentions) Tlc silica gel 60 f254 plate, by Merck Group (2 mentions) Win cats version 1, by CAMAG (2 mentions) Reprostar 3 video documentation unit, by CAMAG (2 mentions) Hptlc system, by CAMAG (2 mentions) Tlc plate heater, by CAMAG (1 mentions) Chromatogram immersion device 3, by CAMAG (1 mentions) Tlc visualizer 2, by CAMAG (1 mentions) Wincat s, by CAMAG (1 mentions) Visioncats, by CAMAG (1 mentions) Wincats software, by CAMAG (1 mentions) 60 f254 aluminium plates, by Merck Group (1 mentions) Tlc plate heater 3, by CAMAG (1 mentions) Reprostar 3, by CAMAG (1 mentions)

Spelling variants of this product

Twin trough glass chamber (25 citations) Twin Trough chromatographic chamber (4 citations) Twin trough developing chamber (3 citations) Twin trough development chamber (2 citations) Twin-trough vertical development chamber (2 citations)

29 protocols using twin trough chamber

Quantification of Luteolin in Herbal Extracts

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Sample preparationTwo milligrams per milliliter stock solution of A. carnosus aqueous and ethanol extracts were prepared by weighing 2 mg of each extract and dissolving in 1 ml methanol.
Standard preparationOne milligram luteolin was weighed and dissolved in 10 ml methanol (analytical grade) to obtain a stock solution having a concentration of 100 µg/ml.
Quantification of luteolin in different extracts/fractions by validated HPTLC method.[16 ]
HPTLC analysis was performed using Linomat V and CAMAG HPTLC software. Ten microliters of each sample were drawn in a Camag 100 µl syringe and applied onto an HPTLC precoated silica gel G60 F₂₅₄ plate (20 cm × 10 cm) using Linomat V semi-automatic applicator. The chromatogram was developed in a Camag twin trough chamber which was presaturated for 20 min at room temperature with mobile phase constituting toluene: Ethyl acetate: Formic acid in the ratio 10:9:1, v/v/v. The plate was subsequently air-dried and scanned at a scanning speed of 20 mm/s in the absorbance mode at a wavelength of 254 nm and 366 nm using Camag TLC scanner III. The slit dimension was maintained at 6.0 mm × 0.45 mm. The peak areas were recorded, and the percentage of luteolin content in each sample was estimated.

Gupta N., Lobo R., Kumar N., Bhagat J.K, & Mathew J.E. (2015). Identity-based High-performance thin Layer Chromatography Fingerprinting Profile and Tumor Inhibitory Potential of Anisochilus carnosus (L.f.) wall Against Ehrlich Ascites Carcinoma. Pharmacognosy Magazine, 11(Suppl 3), S474-S480.

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HPTLC Analysis of Bee and Flower Pollen

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MIX and SSTs (20 mg/mL, 2 µL and 5 µL) of bee pollen samples (STS-TR and STS-SI) and flower pollen sample were applied on 20 cm × 10 cm glass backed HPTLC silica gel 60 F₂₅₄ plates (Merck, Art. No. 1.05642) with a semi-automatic applicator Linomat 5 (Camag, Muttenz, Switzerland) equipped with a 100 µL Hamilton syringe. Applications were performed as 8 mm bands (15.4 mm apart), 8 mm from the bottom of the plate and 15 mm from the left edge. The plate was developed up to 7 cm in a saturated (20 min) twin-trough chamber (20 cm × 10 cm, Camag) with a developing solvent, ethyl acetate–dichloromethane–acetic acid–formic acid–water (100:25:10:10:11, v/v/v/v/v) [27 (link)]. After drying the plate in a stream of cold air, the plate was heated on a TLC plate heater (Camag) at 100 °C for 3 min and immersed into NP derivatization reagent and after drying also into PEG 400 derivatization reagent [28 ] by a Chromatogram Immersion Device III (Camag) for 3 s. Documentation of the plate images was performed using a Visualiser (Camag) after development (at 254 nm and 366 nm), after derivatization with NP reagent (at 366 nm) and after enhancement of the fluorescence by PEG 400 reagent (at 366 nm). The winCATS program was used to operate all the instruments (Camag, Version 128 1.4.8.2031).

Sen N.B., Guzelmeric E., Vovk I., Glavnik V., Kırmızıbekmez H, & Yesilada E. (2023). Phytochemical and Bioactivity Studies on Hedera helix L. (Ivy) Flower Pollen and Ivy Bee Pollen. Antioxidants, 12(7), 1394.

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Thin-Layer Chromatography Analysis of Botanical Extracts

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Plates were pre-washed with methanol—water (4:1 V/V), dried in an oven (Memmert, Schwabach, Germany) for 20 min at 110°C (Morlock, 2014 (link)), and stored wrapped in aluminum foil. All botanical extracts (4 µL/band) were applied as 6 mm bands on a pre-washed plate (Automatic TLC Sampler 4, CAMAG, Muttenz, Switzerland). The plate was developed up to a migration distance of 70 mm with 7 ml ethyl acetate—toluene—formic acid—water (16:4:3:2 V/V/V/V) (Krüger et al., 2017 (link)). Separation was performed in a twin trough chamber (20cm × 10 cm, CAMAG) followed by drying for 4 min with a stream of cold air (hair dryer) and for 20 min in a laminar flow of air (Automated Development Chamber 2, CAMAG). The developed plates were documented at Vis, UV 254 nm, and FLD 366 nm (TLC Visualizer 2, CAMAG). The software winCATS (version 1.4.7.2018) or visionCATS (version 2.5.18262.1, both CAMAG) controlled the instruments.

Schreiner T., Sauter D., Friz M., Heil J, & Morlock G.E. (2021). Is Our Natural Food Our Homeostasis? Array of a Thousand Effect-Directed Profiles of 68 Herbs and Spices. Frontiers in Pharmacology, 12, 755941.

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Optimized Normal Phase TLC Method

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Normal phase thin layer chromatography (NP-TLC) was done on TLC silica gel 60F₂₅₄ plates (E. Merck, Darmstadt, Germany, # 1.05554). Additionally, the TLC silica gel 60F₂₅₄ plates (E. Merck, Germany, # 1.05570) were used for the robustness test. The plates were prewashed with methanol and dried for 24 h at room temperature. Before use, the plates used in NP-TLC were activated at 120 °C for 10 min.
The solutions of pharmaceutical samples and standards of active substances (5 μL) were spotted manually on the chromatographic plates. The mixture of the chloroform–ethanol (96%)-glacial acetic acid in a volume composition of 5:4:0.03 (v/v/v) was used as the mobile phase. A total of 50 mL of the mobile was used in all cases. After saturation of the chamber (20 cm × 20 cm) with the mobile phase vapor for 20 min, the plates were developed vertically at room temperature (20 °C) to a distance of 7.5 cm and then dried for 20 h at room temperature (20 °C) in a fume cupboard. Additionally, a twin-trough chamber of 20 × 10 cm (#0.222.5254, Camag, Muttenz, Switzerland) was used for the robustness test.

Pyka-Pająk A., Dołowy M., Parys W., Bober K, & Janikowska G. (2018). A Simple and Cost-Effective TLC-Densitometric Method for the Quantitative Determination of Acetylsalicylic Acid and Ascorbic Acid in Combined Effervescent Tablets. Molecules, 23(12), 3115.

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HPTLC Fingerprinting for Plant Profiling

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The HPTLC fingerprinting was performed on TLC silica gel plates (20 cm × 10 cm, 60 F254). The HPTLC system was equipped with Linomat 5 (CAMAG, Switzerland) automated spray-on band applicator with a 100 μL Hamilton syringe and operated using Nitrogen gas with the following settings: band length 8 mm, dosage speed 150 Nl⁻¹, inter-band distance of 5 mm, horizontal border-distance of 15 mm, and vertical bottom-distance of 10 mm. The development of the plates was carried out in 10 min with solvent saturation of the twin-trough chamber (CAMAG, Switzerland) at ambient temperature. A solvent system consisting of a) ethyl acetate and hexane (6:3, v/v) b) methanol, chloroform and dichloromethane (4:4:1, v/v/v) was used as a mobile phase. After the mobile phase evaporation, the compounds of interest were viewed under TLC Visualizer (CAMAG, Switzerland) at two UV wavelengths (254 and 366 nm). The images were recorded and R_f values of the markers and the compounds of interest were calculated automatically by winCATS software (version 1.2.3) of CAMAG, Switzerland. All the individuals collected from various populations were subjected to HPTLC screening and additionally, the different plant parts (leaves, roots, bark, fruit pericarp, seed coat and seedling tissues) were analyzed for fingerprinting (TLC & FTIR).

Varun E., Bhakti K., Aishwarya K., Suraj R.H., Jagadish M.R, & Mohana Kumara P. (2023). Rohitukine content across the geographical distribution of Dysoxylum binectariferum Hook F. and its natural derivatives as potential sources of CDK inhibitors. Heliyon, 9(2), e13469.

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TLC Analysis of Compounds

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Linomat 5 applicator (Camag, Switzerland), twin trough chamber (20 × 10 cm; Camag, Switzerland), TLC scanner IV (Camag, Switzerland), win CATS version 1.4.6 software (Camag, Switzerland), Microsyringe (Linomat syringe 659.0014, Hamilton–Bonaduz Schweiz, Camag, Switzerland), UV chamber (Camag, Switzerland), precoated silica gel 60F₂₅₄ aluminium plates (20 × 10 cm, 100 μm thickness; Merck, Darmstadt, Germany) were used in the study.

Patel K.G., Shah P.M., Shah P.A, & Gandhi T.R. (2016). Validated high-performance thin-layer chromatographic (HPTLC) method for simultaneous determination of nadifloxacin, mometasone furoate, and miconazole nitrate cream using fractional factorial design. Journal of Food and Drug Analysis, 24(3), 610-619.

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Thin-Layer Chromatography Analysis

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Linomat 5 applicator (CAMAG, Switzerland), twin trough chamber (20 × 10 cm; CAMAG, Switzerland), TLC scanner IV (CAMAG, Switzerland), winCATS version 1.4.6 software (CAMAG, Switzerland), microsyringe (Linomat syringe 659.0014, Hamilton-Bonaduz Schweiz, CAMAG, Switzerland), UV chamber (CAMAG, Switzerland), and precoated silica gel 60 F₂₅₄ aluminium plates (20 × 10 cm, 100 μm thickness; Merck, Darmstadt, Germany) were used in the study.

Patel N.G., Patel K.G., Patel K.V, & Gandhi T.R. (2015). Validated HPTLC Method for Quantification of Luteolin and Apigenin in Premna mucronata Roxb., Verbenaceae. Advances in Pharmacological Sciences, 2015, 682365.

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HPTLC Analysis of Tinosporaside and Berberine

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HPTLC was performed on 10 cm × 20 cm Higlachrosep plates coated with 0.2 mm layers of nanosilica containing UV 254 fluorescent indicator (S.D. Fine Chemicals, India). Samples (20 μL) were applied as bands 6 mm wide, 11.3 mm apart, 10 mm from the bottom edge, starting 15 mm from the edge of the plate, and by means of a (CAMAG, Switzerland) Linomat applicator fitted with a Hamilton syringe (100 μL). Standards of the markers tinosporaside and berberine were also applied to the plates. The plates were developed to a distance of 8.0 cm with 20 mL chloroform : methanol : water (8 : 2 : 0.2 v/v/v) as mobile phase, in a CAMAG twin-trough chamber previously saturated with mobile phase vapour for 30 min at 24°C. After removal from the chamber, plates were completely dried in air at room temperature (24°C). Densitometric scanning at 220 nm for tinosporaside and at 320 nm for berberine was performed with a CAMAG TLC scanner III with winCATS 3.2.1 software. Photographs were taken by means of a CAMAG Reprostar 3 video documentation unit by illumination at UV366 nm and under visible light after derivatization with anisaldehyde sulphuric acid reagent [33 ].

Choudhry N., Singh S., Siddiqui M.B, & Khatoon S. (2014). Impact of Seasons and Dioecy on Therapeutic Phytoconstituents of Tinospora cordifolia, a Rasayana Drug. BioMed Research International, 2014, 902138.

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Normal Phase Thin Layer Chromatography

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Normal phase thin layer chromatography (NP-TLC) was done on TLC silica gel 60F₂₅₄ plates (E. Merck, Germany, # 1.05554). Additionally, TLC silica gel 60F₂₅₄ plates (E. Merck, Germany, # 1.05570) were used for the robustness test. The plates were prewashed with methanol and dried for 24 h at room temperature. Before use, the plates used in NP-TLC were activated at 120 °C for 10 min.
The solutions of pharmaceutical samples and standards of active substances (5 μL) were spotted on the chromatographic plates. The mixture of cyclohexane:chloroform:methanol:glacial acetic acid (6:3:0.5:0.5, v/v) was used as mobile phase. Of mobile 50 mL was used in all cases. After saturation of twin-trough chamber of 20 cm × 10 cm (#0.222.5254, Camag, Muttenz, Switzerland) with the mobile phase vapor for 30 min., the plates were developed vertically at room temperature (20 °C) to a distance of 7.5 cm and then dried for 20 h at room temperature (20 °C) in a fume cupboard.

Parys W., Pyka-Pająk A, & Dołowy M. (2019). Application of Thin-Layer Chromatography in Combination with Densitometry for the Determination of Diclofenac in Enteric Coated Tablets. Pharmaceuticals, 12(4), 183.

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Simultaneous HPLC Quantification of Olmesartan, Amlodipine and HCTZ

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Working standards of OLM, AML and HCTZ were kindly provided as a gratis sample from Glenmark Generics Limited, Pune, Prudence Pharma Chem, Ankleshwar and Ipca Laboratories Limited, Ratlam, respectively. All solvents and chemicals used were of analytical grade, purchased from Merck Specialities Pvt. Ltd., India. Marketed tablet formulations used in this study were procured from local market; Triolmezest film-coated tablet and Triolmezest 40 tablet, from Sun Pharmaceutical Industries Ltd. and Olmetime-AMH from Mankind Pharma Ltd.
Microsyringe (Linomat syringe 659.0014, Hamilton-Bonaduz Schweiz, Camag, Switzerland), pre-coated silica gel 60F₂₅₄ aluminium plates (10×10 cm, 100μm thickness; Merck, Darmstadt, Germany), Linomat 5 applicator (Camag, Switzerland), twin trough chamber (10×10 cm; Camag, Switzerland), UV chamber (Camag, Switzerland), TLC scanner IV (Camag, Switzerland), win CATS version 1.4.6 software (Camag, Switzerland) were used in the study. All data analysis of experimental design was performed by using the Design-Expert trial version 7.0.0 (Stat-Ease Inc., Minneapolis) and remaining calculations were performed by use of Microsoft Excel 2007 software (Microsoft Corporation, USA).

Solanki T.B., Shah P.A, & Patel K.G. (2014). Central Composite Design for Validation of HPTLC Method for Simultaneous Estimation of Olmesartan Medoxomil, Amlodipine Besylate and Hydrochlorothiazide in Tablets. Indian Journal of Pharmaceutical Sciences, 76(3), 179-187.

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