Acell smart pool

siRNA treatment on transduced cells was performed 72 hr after transduction (and after one cell passage) in 24-well plates for reporter activity assay or 12 well plates for gene expression experiments. HL-1 were transfected by siRNAs as follows: one day after being plated, cells were transfected with 10 nM of small interference RNAs from Dharmacon Acell SMARTpool targeting P54^nrb, PSPC1, SFPQ, hnRNPM, Nucleolin, RPS2, or non-targeting siRNA control (siControl), using INTERFERin (Polyplus Transfection) according to the manufacturer’s recommendations, in DMEM-GlutaMAX +Pyruvate media without penicillin-streptomycin. The media was changed 24 hr after transfection and the cells were incubated 72 hr for the time of transfection at 37 °C with siRNA. For Neat1 knock-down, HL-1 cells were transduced with a pool of 4 gapmers (Qiagen) at 40 nM (10 nM each) and incubated 48 hr after transfection, proceeded essentially as described above (siRNA and gapmer sequences are provided in Supplementary file 2).