Plgel 5 μm mixed c 300 7.5 mm columns

Number-average molecular weight (M n) and polydispersity index (M w/M n) of the PLA was determined using gel permeation chromathography with multi-angle light scattering method (GPC-MALS). An instrumental setup included Agilent HPLC 1100 Series instrument with degasser, pump, autosampler, set of two PLgel 5 μm Mixed-C 300 × 7.5 mm columns (Agilent, USA) thermostated to 25 °C and UV-VIS diode array detector in connection with a DAWN HELEOS II multi-angle laser light scattering detector, ViscoStar-II differential viscometer and Optilab T-rEX refractive index detectors (Wyatt Technology, Germany). Both MALS and RI detectors operated at 658 nm. Tetrahydrofurane was used as the mobile phase at a flow rate of 1 mL/min. Sample in THF (concentration 1 mg/mL) was filtered with 0.22 μm filter and injected in the volume of 100 μL. Astra 6.1 software was used for data collection and analysis and Agilent software was used to control the HPLC. The specific refractive increment dn/dc equal to 0.049 for PLA was used for data processing. The specific refractive index values of polylactide were confirmed by a 100% mass recovery.

The molecular weight, LA/GA molar ratio and PLGA/PEG weight ratio of the copolymer were determined by means of proton nuclear magnetic resonance ¹H NMR spectroscopy on a Bruker (Billerica, MA, USA) ADVANCE III HD 700 MHz instrument in deuterated chloroform (CDCl₃) solvent at 25 °C (Supplementary Figure S1). The number-average molecular weight (M_n) and polydispersity index (M_w/M_n) of the PLGA–PEG–PLGA copolymer were determined via gel permeation chromatography with the multi-angle light scattering method (GPC-MALS) (Supplementary Figure S2). The instrumental setup included an Agilent HPLC 1100 Series instrument with a degasser, pump, autosampler, set of two PLgel 5 μm Mixed-C 300 × 7.5 mm columns (Agilent, Santa Clara, CA, USA) thermostated to 25 °C and a UV-VIS diode array detector connected to a DAWN HELEOS II multi-angle laser light scattering detector, a ViscoStar-II differential viscometer and Optilab T-rEX refractive index detectors (Wyatt Technology, Dernbach, Germany). Tetrahydrofuran was used as the mobile phase at a flow rate of 1 mL·min⁻¹.