Electron microscopy was conducted as previously described [5] (link). After treatment, the samples were dehydrated using acetonitrile and graded methanol, embedded in epoxy resin (EMbed-812; Electron Microscopy Sciences, USA) and polymerized at 70 °C overnight. Hitachi H600 Electron Microscope (Hitachi, Japan) was used to capture the images. The samples were imaged using a Hitachi H600 Electron Microscope (Hitachi, Japan). At least 30 cells in at least 5 randomly selected fields were observed. Echocardiography was performed in all mice at 6 h after reperfusion by echocardiogram (14.0 MHz, Sequoia C512; Acuson, Germany).
H 600 electron microscope
Manufactured by Hitachi
Sourced in Japan
The H-600 electron microscope is a high-performance laboratory instrument designed for advanced materials analysis. It utilizes a focused electron beam to magnify and image microscopic samples, enabling researchers to study the detailed structure and composition of materials at the nanoscale level. The H-600 offers excellent resolution and imaging capabilities, making it a valuable tool for a wide range of scientific and industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
Embed 812, by Electron Microscopy Sciences (9 mentions)
Eclipse 50i, by Nikon (2 mentions)
Sequoia c512, by Siemens (2 mentions)
Ultracut e, by Leica (1 mentions)
Te2000 u, by Nikon (1 mentions)
Epon 812, by Merck Group (1 mentions)
Durcupan, by Merck Group (1 mentions)
Epoxy resin, by Electron Microscopy Sciences (1 mentions)
Image pro plus 4, by Media Cybernetics (1 mentions)
Bx51 microscope, by Olympus (1 mentions)
Ultramicrotome, by Electron Microscopy Sciences (1 mentions)
Diatome diamond knife, by Electron Microscopy Sciences (1 mentions)
Em uc7 microtome, by Leica (1 mentions)
D galactose, by Merck Group (1 mentions)
Formvar coated copper grids, by Agar Scientific (1 mentions)
Methyl cellulose uranyl acetate, by Merck Group (1 mentions)
Glutaraldehyde, by Merck Group (1 mentions)
Epon araldite, by Merck Group (1 mentions)
Epon araldite, by Electron Microscopy Sciences (1 mentions)
Lr white resin medium grade, by Electron Microscopy Sciences (1 mentions)
60 protocols using h 600 electron microscope
1
Microscopic Analysis of Cardiac Injury
2
Histological and Ultrastructural Analysis of Broiler Thymus
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Six broilers in each group were euthanized at 7, 14 and 21 days of age. The thymuses were fixed in 4% paraformaldehyde (PFA) and routinely processed in paraffin. Thin sections (5 μm) of each tissue were sliced and mounted on glass. Slides were stained with hematoxylin and eosin Y. The histological structures of the tissues were observed and photographed with a digital camera (Nikon, eclipse 50i, Japan).
At the end of the trial, one chick per replicate in each group was euthanized and then immediately necropsied. Small pieces of thymus tissues were rapidly fixed with 2.5% glutaraldehyde and post-fixed in 2% Veronal acetate-buffered OsO4. After dehydration in graded alcohol, the tissues were embedded in Araldite. The blocks were sectioned in a microtome with a glass knife. Sections, 65-75 nm thick, were placed in uncoated copper grids. The sections were stained with uranyl acetate, and post-stained with 0.2% lead citrate. The subcellular structure of thymus was examined with a Hitachi H-600 electron microscope (Japan).
At the end of the trial, one chick per replicate in each group was euthanized and then immediately necropsied. Small pieces of thymus tissues were rapidly fixed with 2.5% glutaraldehyde and post-fixed in 2% Veronal acetate-buffered OsO4. After dehydration in graded alcohol, the tissues were embedded in Araldite. The blocks were sectioned in a microtome with a glass knife. Sections, 65-75 nm thick, were placed in uncoated copper grids. The sections were stained with uranyl acetate, and post-stained with 0.2% lead citrate. The subcellular structure of thymus was examined with a Hitachi H-600 electron microscope (Japan).
3
Electron Microscopy of Rat Lung Tissues
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Rat lung tissues were minced into small pieces and fixed in 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer for 4 h. Tissues were post-fixed in 1% osmium tetroxide in 1% K4Fe(CH)6, dehydrated through graded concentrations of ethanol and propylene oxide, embedded in Epon812 and then sectioned with an ultramicrotome. Longitudinal sections were placed onto copper grids, which were stained with uranyl acetate and lead nitrate and visualized with an H-600 electron microscope (Hitachi Limited, Japan).
4
Ultrastructural Analysis of Cellular Morphology
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The morphology of cells in different groups was observed by microscopy. In addition, the ultrastructure of cells was observed using transmission electron microscopy (TEM). Briefly, cells were fixed for 4 h at 4 °C in 5 % glutaraldehyde, washed three times in 0.1 mol/L phosphate buffered saline (PBS), post-fixed for 2 h at 4 °C in 2 % osmium tetroxide, dehydrated in a graded series of ethanols and embedded in Epon 812. Tissue was cut into ultrathin sections (75 nm) and then stained with uranyl acetate and lead citrate. Sections were viewed with a HITACHI H-600 electron microscope at 80 kV (HITACHI, Tokyo, Japan).
5
Electron Microscopy Tissue Fixation
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After treatment, cells and tissues were immediately fixed at 4°C with 2% glutaraldehyde in 0.1 mol/L sodium cacodylate buffer and postfixed for 1 hour on ice with 1% osmium tetroxide. The cells and tissues were rinsed with distilled water and dehydrated using acetonitrile and graded methanol (50%, 20 minutes; 70%, 20 minutes; 95%, 20 minutes; and 100% 3×, 20 minutes), and then were embedded in epoxy resin (EMbed‐812; Electron Microscopy Sciences, USA) and polymerized at 70°C overnight. Thin (60 nm) sections were cut, and the sections were stained with lead citrate and uranyl acetate. The samples were imaged using a Hitachi H600 Electron Microscope (Hitachi, Japan). At least 30 cells in at least 5 randomly selected fields were observed.
6
Prostate Tissue Ultrastructural Analysis
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Prostate tissues were fixed, embedded, and processed for EM [48 (link)] and observed with a Hitachi H-600 electron microscope. At least 300 cells were quantified. Mitochondria with any of the following criteria were used for mitochondrial damage: mitochondrial swelling, mitochondria with loss of cristae, degeneration of mitochondria, lysosomal degradation of mitochondria, vacuolization in mitochondria. Cytoplasm with any of the following criteria were used for cytoplasmic damage: intracytoplasmic vacuolization, intracellular edema, disruption of cell membranes. Cells with at least 50% mitochondrial and/or cytoplasmic damage were considered damaged cells. Damaged cells were counted and reported as % of damaged cells.
7
Mitochondrial Ultrastructure in Ischemia
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Transmission electron microscopy (TEM) was used to observe the structure of mitochondria in response to ischemic injury. For the electron microscopy, samples were dehydrated using acetonitrile and graded methanol, embedded in epoxy resin (EMbed-812; Electron Microscopy Sciences, United States), and polymerized at 70°C overnight. Hitachi H600 Electron Microscope (Hitachi, Japan) was used to capture the images.
8
Histological and Ultrastructural Analysis of Bursa of Fabricius in Broilers
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Six broilers in each group were euthanized at 7, 14 and 21 days of age. The BF were fixed in 4% paraformaldehyde (PFA) and routinely processed in paraffin. Thin sections (5 μm) of each tissue were sliced and mounted on glass. Slides were stained with hematoxylin and eosin Y. The histological structures of the tissues were observed and photographed with a digital camera (Nikon, eclipse 50i, Japan).
At the end of the trial, one chick per replicate in each group was euthanized and then immediately necropsied. Small pieces of BF tissues were rapidly fixed with 2.5 % glutaraldehyde and post-fixed in 2% Veronal acetate-buffered OsO4. After dehydration in graded alcohol, the tissues were embedded in Araldite. The blocks were sectioned in a microtome with a glass knife. Sections, 65-75 nm thick, were placed in uncoated copper grids. The sections were stained with uranyl acetate, and post-stained with 0.2% lead citrate. The subcellular structure of BF was examined with a Hitachi H-600 electron microscope (Japan).
At the end of the trial, one chick per replicate in each group was euthanized and then immediately necropsied. Small pieces of BF tissues were rapidly fixed with 2.5 % glutaraldehyde and post-fixed in 2% Veronal acetate-buffered OsO4. After dehydration in graded alcohol, the tissues were embedded in Araldite. The blocks were sectioned in a microtome with a glass knife. Sections, 65-75 nm thick, were placed in uncoated copper grids. The sections were stained with uranyl acetate, and post-stained with 0.2% lead citrate. The subcellular structure of BF was examined with a Hitachi H-600 electron microscope (Japan).
9
Ultrastructural Analysis of Propofol-Induced Mitochondrial Dynamics
10
Ultrastructural Analysis of Halophilic Cells
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H. hispanica parental and mutant cells in 3.4 M NaCl-containing buffer were fixed in 2.5% glutaraldehyde in 0.1 M phosphate buffer, pH 7.0, for 4 h or overnight at 4 °C, washed three times in 0.1 M phosphate, postfixed in 1% osmium tetroxide in 0.1 M phosphate for 2 to 4 h, incubated in 30%, 50%, 70%, 85%, 95%, and 100% methanol for 15 to 20 min at each concentration, and post-fixed in 2% uranyl acetate-30% methanol. The cells were rinsed, dehydrated, and embedded in Epson 812 for the floating sheet method. Sections were examined with an H-600 electron microscope (Hitachi).
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