Human broblasts (ATCC, CRL-2097) were grown in Fibroblast Medium (FM) including DMEM (Thermo Fisher Scienti c) supplemented with 10% FBS (NATOCOR), 1% GlutaMAX (Thermo Fisher Scienti c), 1% non-essential amino acids (Thermo Fisher Scienti c), 10 ng/ml bFGF (Peprotech), 1% penicillinstreptomycin (Thermo Fisher Scienti c), 0.1% β-mercaptoethanol. Human astrocytes (HA1800, Sciencell) were cultured with AM medium (Catalog #1801 Sciencell). Human ESCs and iPSCs were cultured on Matrigel (Catalog # 365230, Corning) coated plate with E8 medium including 100 ng/ml bFGF (Peprotech), 1.74 ng/ml TGFβ and E6 medium which contains DMEM/F12 (Catalog # 11330032 Gibco) supplement with 13.6 µg/ml sodium selenium (Sigma), 1 mg/ml sodium chloride (Sigma), 64 µg/ml L-Ascorbic acid 2-phosphate (Sigma), 20 µg/ml recombinant human insulin (Sigma) and 10 µg/ml human holo-transferrin (Sigma), 1% penicillin-streptomycin (Thermo Fisher Scienti c).
Ha1800
Manufactured by ScienCell
Sourced in United States
The HA1800 is a laboratory device designed for the detection and measurement of hyaluronic acid (HA) levels. It utilizes a spectrophotometric method to quantify HA concentrations in various biological samples. The core function of the HA1800 is to provide researchers and scientists with an accurate and reliable tool for HA analysis.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions)
Dmem f12, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Dmem medium, by Thermo Fisher Scientific (2 mentions)
Matrigel, by Corning (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Basic fibroblast growth factor (bfgf), by Thermo Fisher Scientific (1 mentions)
L ascorbic acid 2 phosphate, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Natocor (1 mentions)
Human holo transferrin, by Merck Group (1 mentions)
Recombinant human insulin, by Merck Group (1 mentions)
Sodium selenium, by Merck Group (1 mentions)
Capmatinib hy 13404, by MedChemExpress (1 mentions)
Tryple, by Thermo Fisher Scientific (1 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
14 protocols using ha1800
1
Culturing Human Cell Lines
2
Comprehensive Cell Line Characterization
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Human bronchial epithelial cells (16HBE cells), human lung fibroblasts (HFL1 cells), human monocyte cells (THP-1 cells), normal bronchial epithelial cells (BEAS-2B cells) and lung cancer cell lines (H1299, H2030, PC9, H1975, H460, and H226 cells) were purchased from the Chinese Academy of Medical Sciences (Beijing, China). The brain metastatic lung cancer cell line H2030-BrM and PC9-BrM was generated by injecting H2030 cells and PC9 cells into the left ventricle of immunodeficient mice and isolating the metastatic cells from harvested areas of brain metastases. Human lung microvascular endothelial cells (hPMECs), human brain microvascular endothelial cells (hBMVECs) and human astrocytes (HA-1800) were purchased from Sciencell (Sciencell, USA) and cultured in the appropriate medium recommended by the manufacturer. The above cell types were cultured at 37°C in humidified air with 5% CO2. The different cell types were authenticated by short tandem repeat profiling and tested for mycoplasma contamination.
JNK-IN-8 (HY-13319), Crizotinib (HY-50878), Anetumab (HY-P99352) and Capmatinib (HY-13404) were purchased from MedChemexpress (USA).
JNK-IN-8 (HY-13319), Crizotinib (HY-50878), Anetumab (HY-P99352) and Capmatinib (HY-13404) were purchased from MedChemexpress (USA).
3
Culturing and Maintaining Human Astrocytes
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Human astrocytes (HA1800) were purchased from ScienCell (CA, USA) and were cultured and maintained in a growth medium comprising GibcoDulbecco’s Modified Eagle Medium: F-12 (DMEM/F12, GIBCO) supplemented with 10% fetal bovine serum (FBS, GIBCO) and 1% penicillin/streptomycin (GIBCO). Cells cultured to greater than 90% confluence were dissociated using TrypLE (GIBCO), centrifuged at 1000 rpm for 5 min, resuspended, and replated in fresh growth medium. Cells were maintained at 37 °C under a humidified atmosphere of air and 5% CO2.
4
Culturing Human Astrocyte Cell Line
5
Glioblastoma Cell Culture and Maintenance
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The human glioblastoma cell lines, U251 and U87, were purchased from the Cancer Institute & Hospital, Chinese Academy of Medical Sciences. The human normal astrocyte cells HA1800 were bought from SCIENCELL. Cells were maintained with DMEM supplemented with 10% FBS, streptomycin (100 μg/mL) and penicillin (100 U/mL). The cells were cultured at 37°C in an incubator with a humidified atmosphere of 5% CO2.
6
Culturing Human Glioblastoma and Astrocyte Cells
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Human GBM cell lines were purchased from Sigma (U251) or ATCC (U118). U251 cells were cultured in GBM culture medium, which included MEM (GIBCO), 0.2% penicillin/streptomycin (GIBCO), 10% FBS (GIBCO), 1mM Sodium Pyruvate (GIBCO), 1% Non Essential Amino Acids (NEAA, GIBCO), and 1 x GlutMAX (GIBCO). U118 cells were cultured in culture medium including DMEM (GIBCO), 10% FBS and 1% penicillin/streptomycin. Human astrocytes were purchased from ScienCell (HA1800, San Diego, USA).
Human astrocytes were cultured in human astrocyte medium, which included DMEM/F12 (GIBCO), 10% FBS, 3.5 mM Glucose (Sigma), and 0.2% penicillin/streptomycin, supplemented with B27 (GIBCO), N2 (GIBCO), 10 ng/ml fibroblast growth factor 2 (FGF2, Invitogen), and 10 ng/ml epidermal growth factor (EFG, Invitrogen).
For subculture, cells were Trypsinized by 0.25% Trypsin (GIBCO) or TrypLE Select (Invitrogen), centrifuged for 5 min at 800 rpm, re-suspended and plated in corresponding culture medium with a split ratio around 1:4. Cells were maintained at 37°C in humidified air with 5% CO 2 .
Human astrocytes were cultured in human astrocyte medium, which included DMEM/F12 (GIBCO), 10% FBS, 3.5 mM Glucose (Sigma), and 0.2% penicillin/streptomycin, supplemented with B27 (GIBCO), N2 (GIBCO), 10 ng/ml fibroblast growth factor 2 (FGF2, Invitogen), and 10 ng/ml epidermal growth factor (EFG, Invitrogen).
For subculture, cells were Trypsinized by 0.25% Trypsin (GIBCO) or TrypLE Select (Invitrogen), centrifuged for 5 min at 800 rpm, re-suspended and plated in corresponding culture medium with a split ratio around 1:4. Cells were maintained at 37°C in humidified air with 5% CO 2 .
7
Culturing Human Cell Lines
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Human cell lines HA1800, U87, T98G, U118, A172, U251, and HMC3 were purchased from ScienCell Research Laboratory, Cell Bank of the Chinese Academy of Sciences and ATCC, and cultured in Dulbecco’s modified Eagle’s medium (DMEM) medium (Invitrogen, Thermo Fisher Scientific, Inc., the United States). All the culture media was supplement by 10% fetal bovine serum (FBS) (Gibco), Penstrep (Gibco), GlutaMAX (Gibco), and MEM non-Essential Amino Acids (MEM-NEAA) (Gibco) following the instruction of the manufacturer. These cells were all cultured in an incubator with 5% CO2 at 37°C.
8
Isolation and Culture of Primary Astrocytes and Microglia
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The primary astrocytes (HA #1800) and microglia (HM #1900) types are isolated from normal human brain and are commercially available (Sciencell Research Laboratories, Carlsbad, CA, USA). Cells were grown in the specified media as recommended by the manufacturer. Depending upon the experiments, cells were cultured at a concentration of 5 × 105 cells/ml in six-well plates (for transfection experiment) or 1 × 106 cells/ml and allow them to reach at least 70% confluency before any further treatment.
9
Astrocyte Cell Line Infection
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10
Glioma Cell Line and Astrocyte Culture
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Glioma cell line U87MG was obtained from American Type Culture Collection and cultured in D-MEM supplemented with 10% fetal bovine serum; human primary astrocytes (HA1800) were obtained from ScienCell Research Laboratories (Carlsbad, CA) and were cultured according to the provider's guidelines for no more than 10 passages in astrocyte media (AM 1801). The U87MG-Cx43-T154A cell line was obtained by infection with retroviral pMSCV-puro vectors with empty plasmid or mutant Cx43 (T154A) as described previously [33 (link)]. Stable cell lines were selected in 0.75 μg/ml puromycin.
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