Trypsin edta

Manufactured by Sartorius

Sourced in Israel, United States

Trypsin-EDTA is a cell culture reagent used for the dissociation and detachment of adherent cells. It contains the proteolytic enzyme trypsin and the chelating agent EDTA, which together help to break down the cell-to-cell and cell-to-substrate adhesions, allowing cells to be harvested and sub-cultured. This product is commonly used in various cell culture applications.

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Lab products found in correlation

Fetal bovine serum (fbs), by Sartorius (12 mentions) Penicillin streptomycin, by Sartorius (11 mentions) L glutamine, by Sartorius (10 mentions) Rpmi 1640 medium, by Sartorius (4 mentions) Dimethyl sulfoxide (dmso), by Merck Group (4 mentions) Dulbecco modified eagle medium (dmem), by Sartorius (4 mentions) Rpmi 1640, by Sartorius (3 mentions) Fetal calf serum (fcs), by Sartorius (3 mentions) Antibiotic antimycotic solution, by Sartorius (3 mentions) Phosphate buffered saline (pbs), by Sartorius (3 mentions) Penicillin streptomycin solution, by Sartorius (3 mentions) Knockout dulbecco s modified eagle s medium, by Thermo Fisher Scientific (3 mentions) Penicillin, by Thermo Fisher Scientific (3 mentions) Fibroblast growth factor 2 (fgf2), by Thermo Fisher Scientific (3 mentions) Glutamine, by Merck Group (3 mentions) M199 medium, by Sartorius (2 mentions) Il 1β, by Thermo Fisher Scientific (2 mentions) Thiazolyl blue tetrazolium bromide, by Merck Group (2 mentions) Penicillin, by Sartorius (2 mentions) Streptomycin, by Sartorius (2 mentions)

Spelling variants of this product

Trypsin (101 citations) Trypsin-EDTA solution (15 citations) Trypsin EDTA Solution B (12 citations) Trypsin–EDTA solution C (10 citations) Recombinant trypsin EDTA solution (8 citations) Trypsin EDTA solution A (4 citations) Trypsin–EDTA (0.25%) (2 citations)

66 protocols using trypsin edta

Culturing Diverse Cell Lines for Research

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ARP1 and H929 human MM cell lines and the murine MM cell line, 5TMM3VT, were purchased from the Cell Resource Center of the Shanghai Institute of Biochemistry and Cell Biology at the Chinese Academy of Sciences. Cells were cultured in RPMI 1640 (Biological Industries, Beit Haemek, Israel), supplemented with 10% fetal bovine serum (FBS; Biological Industries, Israel) and 1% penicillin/streptomycin (P/S).
The pre-osteoblast murine cell line MC3T3-E1 was purchased from the Cell Resource Center of the Shanghai Institute of Biochemistry and Cell Biology at the Chinese Academy of Sciences, and cultured in alpha modified Eagle’s medium (α-MEM; Biological Industries, Israel) supplemented with 10% FBS (Biological Industries, Israel) and 1% (P/S). Cells were routinely subcultured using 0.05% trypsin/EDTA (Biological Industries, Israel) upon reaching 80–90% confluence.
The pre-osteoclast murine cell line Raw264.7 was purchased from the ATCC and cultured in Dulbecco’s modified Eagle medium (DMEM; Biological Industries, Israel), supplemented with 10% FBS (Biological Industries, Israel) and 1% P/S. After reaching 80–90% confluence, cells were routinely subcultured using 0.05% trypsin/EDTA (Biological Industries, Israel). All cells were maintained at 37°C in a humidified atmosphere of 5% CO2. Medium was changed every 2 days.

Hou J., Qian J., Li Z., Gong A., Zhong S., Qiao L., Qian S., Zhang Y., Dou R., Li R., Yang Y, & Gu C. (2020). Bioactive Compounds from Abelmoschus manihot L. Alleviate the Progression of Multiple Myeloma in Mouse Model and Improve Bone Marrow Microenvironment. OncoTargets and therapy, 13, 959-973.

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Evaluating Klotho-Mediated Cytoprotection

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Culture plasticware was from TPP (Trasadingen, Switzerland). M199 medium, fetal calf serum (FCS), and trypsin‐EDTA were from Biological Industries (Beit‐Haemek, Israel). Human r‐klotho and IL‐1β were purchased from Abcam (ab84072; Cambridge, UK) and Peprotech (London, UK), respectively. Ang‐(1‐7) and the Mas antagonist A779 were purchased from Bachem (Bubendorf, Switzerland). Sn‐PP and sulforaphane were from Frontier Scientific (Logan, UT, USA) and LKT Laboratories (Minnesota, USA), respectively. All other reagents were purchased from Sigma (St. Louis, MO, USA) unless otherwise stated.

Romero A., San Hipólito‐Luengo Á., Villalobos L.A., Vallejo S., Valencia I., Michalska P., Pajuelo‐Lozano N., Sánchez‐Pérez I., León R., Bartha J.L., Sanz M.J., Erusalimsky J.D., Sánchez‐Ferrer C.F., Romacho T, & Peiró C. (2019). The angiotensin‐(1‐7)/Mas receptor axis protects from endothelial cell senescence via klotho and Nrf2 activation. Aging Cell, 18(3), e12913.

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Investigating Angiotensin Receptor Modulators

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Culture plastic ware was from TPP (Trasadingen, Switzerland). Dulbecco’s Modified Eagle’s Medium (DMEM), fetal calf serum (FCS), and trypsin-EDTA were from Biological industries (Beit-Hamek, Israel). IL-1β was from Peprotech (London, UK), while the Mas antagonists A779 and D-Pro⁷-Ang-(1-7) were purchased Bachem (Bubendorf, Switzerland) and Biosyntan (Berlin, Germany), respectively. Anakinra was purchased from Swedish Orphan Biovitrum AB (Stockholm, Sweden). Ang-(1-7), Ang II, losartan, apocynin, and pyrrolidine dithiocarbamate (PDTC) were purchased from Sigma (St. Louis, MO, USA).

Villalobos L.A., San Hipólito-Luengo Á., Ramos-González M., Cercas E., Vallejo S., Romero A., Romacho T., Carraro R., Sánchez-Ferrer C.F, & Peiró C. (2016). The Angiotensin-(1-7)/Mas Axis Counteracts Angiotensin II-Dependent and -Independent Pro-inflammatory Signaling in Human Vascular Smooth Muscle Cells. Frontiers in Pharmacology, 7, 482.

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Quantitative Analysis of Peptide Affinity to Rabbit BMSCs

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The rabbit BMSCs were washed twice with PBS (cat. no. 02-024-1A; Biological Industries, Ltd.) and dissociated with 0.25% trypsin-EDTA (cat. no. 25200-056; Gibco; Thermo Fisher Scientific, Inc.). The cell suspension was centrifuged at 250×g for 5 min at room temperature to collect cell sedimentation. The cells were further washed twice with PBS and then incubated with 10 μM FITC-labeled peptides dissolved in PBS for 1 h at 37 °C to allow cell binding. The affinity of the peptides towards rabbit BMSCs was analyzed quantitatively through flow cytometer (FCM; BD LSR Fortessa; Becton, Dickinson and Company, Franklin Lakes, NJ, USA) at a wavelength of 488 nm and FlowJo 7.6.1 software (Tree Star, Inc., Ashland, OR, USA).

Wang G., Li Y., Sun T., Wang C., Qiao L., Wang Y., Dong K., Yuan T., Chen J., Chen G, & Sun S. (2019). BMSC affinity peptide-functionalized β-tricalcium phosphate scaffolds promoting repair of osteonecrosis of the femoral head. Journal of Orthopaedic Surgery and Research, 14, 204.

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Anticancer Drug Sensitivity Assay

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RPMI 1640 medium, fetal bovine serum (FBS), antibiotic-antimycotic solution, penicilin-streptomicin solution, L-glutamine and trypsin/EDTA were purchased from Biological Industries (Beit Haemek, Israel). Rhodamine 123, DMSO and thiazolyl blue tetrazolium bromide (MTT) were purchased from Sigma-Aldrich Chemie GmbH (Hamburg, Germany). FITC-conjugated anti-P-glycoprotein antibody was obtained from BD Biosciences (Plymouth, UK). Annexin V-FITC apoptosis detection kit with Propidium Iodide was purchased from Abcam (Cambridge, UK).

Dinić J., Podolski-Renić A., Jovanović M., Musso L., Tsakovska I., Pajeva I., Dallavalle S, & Pešić M. (2019). Novel Heat Shock Protein 90 Inhibitors Suppress P-Glycoprotein Activity and Overcome Multidrug Resistance in Cancer Cells. International Journal of Molecular Sciences, 20(18), 4575.

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Cell Culture Media and Reagents

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Phosphate buffered saline (PBS), Dulbecco's Modified Eagle's Medium (DMEM), fetal calf serum (FCS), L-glutamine, antibiotics combination of streptomycin and penicillin, and Trypsin-EDTA were all purchased from Biological Industries (Beit Haemek, Israel). BSA and Tween-20 were purchased from ICN Biomedicals, Inc. (Aurora, OH, USA).

Medina L., Rabinovich A., Piura B., Dyomin V., Shaco Levy R, & Huleihel M. (2014). Expression of IL-18, IL-18 Binding Protein, and IL-18 Receptor by Normal and Cancerous Human Ovarian Tissues: Possible Implication of IL-18 in the Pathogenesis of Ovarian Carcinoma. Mediators of Inflammation, 2014, 914954.

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Cell Viability Assay of MDA-MB-231 and L929 Cells

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Human breast cancer cell line MDA-MB-231 (HTB-26) and mouse subcutaneous connective tissue cell line L929 (CRL-6364) were obtained from ATCC. Dulbecco’s modified Eagle’s medium, fetal bovine serum and phosphate buffer saline were supplied from PAA Ltd. (France). Trypsin-EDTA was purchased from Biological Industries Ltd. (Haemek, Israel). l-glutamine–penicillin–streptomycin solution was bought from Sigma-Aldrich. XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) reagent was purchased from Roche Diagnostic. Both cell lines were maintained in DMEM containing 10 % FBS, 1 % l-glutamine, 100 IU/mL penicillin, and 10 mg/mL streptomycin and cultured in a humidified atmosphere with 5 % CO₂ at 37 °C. The cells were used for the experiments when they reached 85–90 % confluence.

Eruygur N., Ucar E., Ataş M., Ergul M., Ergul M, & Sozmen F. (2019). Determination of biological activity of Tragopogon porrifolius and Polygonum cognatum consumed intensively by people in Sivas. Toxicology Reports, 7, 59-66.

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Haploid Hpescs Mutant Library Protocol

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Our recently established mutant library on haploid hpESCs (based on hpES10 cell line)^{28 (link)} was maintained for 4 weeks and frozen. Briefly, haploid-enriched cultures of hpES10 cell line were infected with a lentivirus library containing 181,131 sgRNAs at a multiplicity of infection of 0.3. Infected cells were selected with puromycin (Sigma) for 7 days. The mutant population was then expanded for about two weeks before it was frozen. The library was thawed and cultured at 37 °C with 5% CO₂ in feeder-free conditions using Matrigel-coated plates (Corning) and mTeSR1 medium (STEMCELL Technologies) supplemented with 10 μM ROCK inhibitor (Y27632, Stemgent) for 1 day after thawing or splitting. Before reaching confluency, cells were passaged using Trypsin-EDTA (Biological Industries).

Bar S., Vershkov D., Keshet G., Lezmi E., Meller N., Yilmaz A., Yanuka O., Nissim-Rafinia M., Meshorer E., Eldar-Geva T, & Benvenisty N. (2021). Identifying regulators of parental imprinting by CRISPR/Cas9 screening in haploid human embryonic stem cells. Nature Communications, 12, 6718.

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Measurement of Cellular ROS Levels

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Cells were digested with trypsin-EDTA (Biological Industries, Shanghai, China) and transferred to Eppendorf tubes. The Eppendorf tubes were then centrifuged for 1 min at 12,000× g, and the supernatant was discarded. ROS levels were then detected using the H₂DCFDA (2′,7′-dichlorofluorescin diacetate) probe (Sigma-Aldrich, Milpitas, CA, USA). The myotubes were incubated with H₂DCFDA (10 μM) for 30 min at 37 °C, and the fluorescence intensity of H₂DCFDA was detected using the Multilabel Reader (Enspire, Turku, Finland).

Li Y., Zhang S., Huang C, & Lin D. (2023). NMR-Based Metabolic Profiling of the Effects of α-Ketoglutarate Supplementation on Energy-Deficient C2C12 Myotubes. Molecules, 28(9), 3840.

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Isolation and Culture of Human Skeletal Muscle Progenitor Cells

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Human tissue sampling of the present study was reviewed and approved by the National Taiwan University Hospital Institutional Review Board for clinical investigation and collected after written informed consent from all participating subjects. The process of isolation and culture of primary HSMPCs was described previously.25 Briefly, human skeletal muscle biopsies (~0.2 g) were collected from 10 rectus muscles of patients under orthopaedic surgery (mean age, 64 years; range, 34 to 81 years, both male and female). The minced segregate muscle was processed for HSMPC isolation within a few hours by serial enzymatic dissociation (0.5% type XI collagenase for 1 h, Sigma; 2.4 IU/mL dispase for 45 min, Invitrogen; 0.2% trypsin–EDTA for 15 min, Biological Industries). The cell suspension was passed through a 70 μm mesh filter and seeded in a collagen‐coated dish in proliferative growth medium [20% foetal bovine serum (Gibco), 1% PSA (Biological Industrial), and 5 ng/mL basic fibroblast growth factor (ProSpec, East Brunswick, NJ, USA) in Ham's F‐10 (Gibco)]. After serial transfers and pre‐plates based on different adhesion abilities between fibroblasts and muscle‐derived progenitor cells as described previously,25 the isolated HSMPCs with desmin‐positive (as a myogenic progenitor marker)28 staining were used for myogenic differentiation.

Chiu H., Chiu C., Yang R., Chan D., Liu S, & Chiang C. (2018). Preventing muscle wasting by osteoporosis drug alendronate in vitro and in myopathy models via sirtuin‐3 down‐regulation. Journal of Cachexia, Sarcopenia and Muscle, 9(3), 585-602.

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