Restriction enzymes

A tetrahydroquinoline (THQ)‐type compound (C₂₃H₁₉BrF₂N₂O) was synthesized as previously described 40, 41. Dimethyl sulfoxide (DMSO), tebufenozide, methoxyfenozide, chromafenozide, corticosterone, hydrocortisone, and testosterone were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Dithiothreitol (DTT), 17β‐estradiol (E2), and progesterone were obtained from Nacalai Tesque (Kyoto, Japan). 20‐Hydroxyecdysone (20E), ecdysone, ponasterone A (Pon A), aldosterone, halofenozide, and o‐nitrophenyl‐β‐d‐galactopyranoside (ONPG) were purchased from Sigma Aldrich Chemical Co. (St. Louis, MO, USA). Restriction enzymes, DNA modification enzymes, and other chemicals were obtained from Wako Pure Chemical Industries, Ltd., TaKaRa Bio Inc. (Otsu, Japan), or TOYOBO Co., Ltd. (Osaka, Japan).

Dimethyl sulfoxide (DMSO) and dithiothreitol (DTT) were obtained from Nacalai Tesque (Kyoto, Japan). JH III, (S)‐hydroprene, methoprene, pyriproxyfen, and o‐nitrophenyl‐β‐D‐galactopyranoside (ONPG) were purchased from Sigma‐Aldrich Chemical Co. (St. Louis, MO, USA). Methyl farnesoate was obtained from Echelon Biosciences Inc. (Salt Lake City, UT, USA). Fenoxycarb was supplied from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). JH 0, I, and II were synthesized as previously described [46 (link)]. Restriction enzymes, DNA modification enzymes, and other chemicals were obtained from Wako Pure Chemical Industries, Ltd. (Osaka Japan), Nacalai Tesque, Sigma‐Aldrich Chemical Co., TaKaRa Bio Inc. (Otsu, Japan), or TOYOBO Co., Ltd. (Osaka, Japan).