Poly ethylene glycol methyl ether acrylate peg ma

Manufactured by Merck Group

Sourced in Germany, United States

Poly(ethylene glycol) methyl ether acrylate (PEG-MA) is a polymeric material commonly used in laboratory applications. It is a polyether-based monomer with an acrylate functional group. PEG-MA can be used as a building block for the synthesis of various polymeric structures.

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3 protocols using poly ethylene glycol methyl ether acrylate peg ma

Synthesis and Characterization of NIPA Hydrogels

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N-isopropyl acrylamide (NIPA, 97%, Sigma Aldrich, Sternheim, Germany), potassium persulfate (KPS, 98%, Sigma Aldrich, Sternheim, Germany), dihydrochloride 2,2′-azobis (2-methyl propionamidine) (AMP, granules, 97%, Sigma Aldrich, Sternheim, Germany), N,N-methylene bisacrylamide (MBA, 99%, Sigma Aldrich, Sternheim, Germany), poly(ethylene glycol) methyl ether acrylate (PEG-MA, 99%, average Mn 500, Sigma Aldrich, Sternheim, Germany), and N-tertbutyl acrylamide (NTB, 99%, Acros Organics, Geel, Belgium) were obtained from industrial and commercial sources and were used without further purification. The dialysis bag with a molecular mass cut-off MWCO 12,000–14,000 Da was obtained from Visking Medicell International Ltd. (London, UK). Deionised water was obtained from an ionic column and conformed to the European Pharmacopoeia monograph on purified water.

Gasztych M., Malamis A, & Musiał W. (2024). The Influence of Initiators, Particle Size and Composition on the Electrokinetic Potential of N-(Isopropyl)acrylamide Derivatives. Polymers, 16(7), 907.

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Synthesis and Characterization of Polymeric Hydrogel

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Carbon disulfide, chloroform, dimethyl sulphoxide (DMSO), acetonitrile, acetone, SOCl₂, dichloromethane, tetrahydrofuran, ethyl acetate. tetrabutylammonium hydrogen sulfate, o-phenylenediamine, 2,2′-Azobis(2-methylpropionitrile) (AIBN), propynol ethoxylate, N,N-dimethyl-p-phenylene diamine 1-ethyl-3-(3-(dimethylamino)propyl) carbodiimide (EDC·HCl), Tris(2-carboxyethyl) phosphine hydrochloride (TCEP-HCl), 3,3′-dithiobis(propanoic dihydrazide) (DTPHY) were purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Poly(ethylene glycol) methyl ether acrylate(pegma, Mn = 480), HA sodium salt, lipopolysaccharide (LPS) and griess reagent were purchased from Sigma-Aldrich (St. Louis, MO, USA), WSP-5 and DAF-2 were obtained from APExBIO Technology LLC (Houston, USA). RANKL and m-CSF were purchased from Peprotech Inc.

Geng W., Zhao J., Tao B., Yang Y., Duan Q., Gao P., He T., Liu S., Feng Q., Zhao P, & Cai K. (2024). Regulation of rheumatoid arthritis microenvironment via a self-healing injectable hydrogel for improved inflammation elimination and bone repair. Bioactive Materials, 36, 287-300.

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DNA Functionalization and Cell Culture Protocol

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Custom made DNA were purchased from Integrated DNA technologies (IDT, Coralville, IA USA); see the sequences in Table 1 and applied concentrations are specified in the Supporting Information. MCF-7 cell line, Eagle’s Minimum Essential Medium (EMEM) cell culture medium, PBS were purchased from American Type Culture Collection (ATCC), VA, USA. Trypsin (E.C. 3.4.21.4), fetal bovine serum (FBS), fetal calf serum (FCS), Trypan Blue (stain dye), (1-ethyl-3[3-(dimethylamino)-propyl] carbodiimide (EDC), N-hydroxysuccimide (NHS), Tween-20, 2-(N-morpholino)ethanesulfonic acid (MES-buffer), (4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid (HEPES-buffer), silicon tetraethoxide (TEOS), (3-aminopropyl)triethoxysilane (APS), α-bromoisobutyryl bromide (BIB), ethyl α-bromoisobutyrate (EBIB), N,N,N′,N″,N″-pentamethyldiethylenetriamine (PMDTA), and other standard organic and inorganic materials and reactants were obtained from Sigma-Aldrich or J.T. Baker and used without further purification. tert-Butyl acrylate (TBA) and poly(ethylene glycol) methyl ether acrylate (PEGMA) (average molecular mass 480 g/mol) were purchased from Sigma-Aldrich and purified using a flash-chromatography column containing inhibitor removers (Sigma #311340 and Sigma #311332). All solutions for the experiments were prepared using ultrapure water (18.2 MΩ∙cm; Barnstead NANOpure Diamond).

Bakshi S.F., Guz N., Zakharchenko A., Deng H., Tumanov A.V., Woodworth C.D., Minko S., Kolpashchikov D.M, & Katz E. (2018). Nanoreactors Based on DNAzyme-Functionalized Magnetic Nanoparticles Activated by Magnetic Field. Nanoscale, 10(3), 1356-1365.

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