Mlphorbol 12 myristate 13 acetate pma

Manufactured by Merck Group

Sourced in United States

MLphorbol-12-myristate-13-acetate (PMA) is a chemical compound commonly used as a laboratory reagent. It functions as a protein kinase C activator, which can lead to various cellular responses. This information is provided in a factual and unbiased manner, without any interpretation or extrapolation on its intended use.

Automatically generated - may contain errors

Lab products found in correlation

Golgistop protein transport inhibitor, by BD (1 mentions) Tgf β, by R&D Systems (1 mentions)

Spelling variants of this product

12-myristate 13-acetate (PMA) (6 citations)

2 protocols using mlphorbol 12 myristate 13 acetate pma

DON Induces Inflammatory Response in THP-1 Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols

The in vitro pro-inflammatory effect of
DON on THP-1 macrophages was assessed by measuring the pro-inflammatory
cytokine release following DON exposure. THP-1 cells were seeded at
1.8 × 10⁵ cells/well in a 12-well plate with 50 ng/mL
phorbol-12-myristate-13-acetate (PMA) (Sigma-Aldrich, St Louis, MO,
USA) for 24 h. After PMA induced differentiation, THP-1 macrophages
were washed once and supplied with the culture medium for an additional
24 h. THP-1 macrophages were subsequently exposed to DON (0, 0.25,
0.5, 1, 2.5, and 5 μM) (Sigma-Aldrich) for 24 h in the culture
medium. These concentrations of DON were shown to be noncytotoxic
to THP-1 macrophages (Figure S1). The medium
was collected, and the concentrations of IL-1β were quantified
by an enzyme-linked immune-sorbent assay (ELISA) performed according
to manufacturer’s instructions (Biolegend, San Diego, CA, USA).

Wang J., de Bruijn V., Rietjens I.M., Kramer N.I, & Bouwmeester H. (2023). Use of Physiologically Based Kinetic Modeling to Predict Deoxynivalenol Metabolism and Its Role in Intestinal Inflammation and Bile Acid Kinetics in Humans. Journal of Agricultural and Food Chemistry, 72(1), 761-772.

+ Open protocol

+ Expand

Inducing Inflammatory Cytokines in Tregs

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

To induce the production of inflammatory cytokines, sorted CD27^+/−Tregs were cultured in a medium containing 10 IU/ml IL-2, 10 ng/ml IL-1β, 5
ng/ml IL-6, 25 ng/ml IL-21, 25 ng/ml IL-23, and 5 ng/ml TGF-β (R&D Systems,
Minneapolis, MN, USA) for 7 days. Cells cultured in medium containing only 10
IU/ml IL-2 were used as the non-induction control^{24 (link)}. Sorted Tregs were
cultured in the upper chamber of a transwell system (pore size:0.4 µm) with or
without anti-CD3/CD28 Dynabead-stimulated PBMCs in the lower chamber. Seven days
after stimulation, the Tregs were collected and further incubated with 50 ng/ml
phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich, San Luis, MO USA), 1 µg/ml
ionomycin (Sigma-Aldrich, San Luis, MO USA), and 1 µl/ml GolgiStop protein
transport inhibitor (BD Biosciences, San Diego, CA, USA) for an additional 5 h
prior to cytometric analysis of a percentage of IL-17a-secreting cells.

Cao L., Ma X., Zhang J., Yang M., He Z., Yang C., Li S., Rong P, & Wang W. (2023). CD27-Expressing Xenoantigen-Expanded Human Regulatory T Cells Are Efficient in Suppressing Xenogeneic Immune Response. Cell Transplantation, 32, 09636897221149444.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!