Waters synapt g2 quadrupole time of flight mass spectrometer

The melting point (mp.) values of the prepared compounds were recorded on a Thermocouple digital melting point apparatus (Mettler Toledo LLC, Columbus, OH, USA) and are uncorrected. Their infrared (IR) spectra were recorded using the thin-film method on a Bruker VERTEX 70 FT-IR Spectrometer (Bruker Optics, Billerica, MA, USA) equipped with a diamond attenuated total reflectance (ATR) accessory. The Merck kieselgel 60 (0.063–0.200 mm) (Merck KGaA, Frankfurt, Germany) was used as a stationary phase for column chromatography. The proton (¹H-) and carbon-13 (¹³C-) nuclear magnetic resonance (NMR) spectra of the prepared compounds were obtained as CDCl₃ or dimethyl sulfoxide-d₆ (DMSO-d₆) solutions using Agilent 500 MHz NMR spectrometer (Agilent Technologies, Oxford, UK). The chemical shifts are quoted relative to the tetramethylsilane (TMS) peak as an internal reference standard. The high-resolution mass spectra were recorded at the University of Stellenbosch using a Waters Synapt G2 Quadrupole Time-of-flight mass spectrometer (Waters Corp., Milford, MA, USA) at an ionization potential of 70 eV. The synthesis and analytical data for 2,4-dihydroxy-5-iodoacetophenone, were reported in our previous investigation [23 (link)].

The melting points of the compounds prepared in this investigation were recorded on a Thermocouple digital melting point apparatus (Mettler Toledo LLC, Columbus, OH, USA). Their IR spectra were recorded as powders using a Bruker VERTEX 70 FT-IR Spectrometer (Bruker Optics, Billerica, MA, USA) equipped with a diamond attenuated total reflectance (ATR) accessory. For column chromatography, we used the Merck kieselgel 60 (0.063–0.200 mm) (Merck KGaA, Frankfurt, Germany) as stationary phase. The NMR spectra were obtained as DMSO-d₆ solutions using Varian Mercury 300 MHz NMR spectrometer (Varian Inc., Palo Alto, CA, USA) and the chemical shifts are quoted relative to the residual proton signal in the deuterated solvent. The mass spectra were recorded at an ionization potential of 70 eV using Waters Synapt G2 Quadrupole Time-of-flight mass spectrometer (Waters Corp., Milford, MA, USA) at the University of Stellenbosch. The synthesis and analytical data of compounds 1a–d, which are known have been described before [18 (link)].

The melting point values of the prepared compounds were recorded on a Thermocouple digital melting point apparatus and are uncorrected. Their IR spectra were recorded as powders on a Bruker VERTEX 70 FT-IR Spectrometer (Bruker Optics, Billerica, MA, USA) with a diamond ATR (attenuated total reflectance) accessory by using the thin-film method. Merck kieselgel 60 (0.063–0.200 mm) (Merck KGaA, Frankfurt, Germany) was used as stationary phase for column chromatography. The ¹H-NMR and ¹³C-NMR spectra were obtained as DMSO-d₆ solutions using Agilent 300 MHz NMR (Agilent Technologies, Oxford, UK) spectrometer and the chemical shifts are quoted relative to the TMS peak. The high-resolution mass spectra were recorded at an ionization potential of 70 eV using Waters Synapt G2 Quadrupole Time-of-flight mass spectrometer (Waters Corp., Milford, MA, USA) at the University of Stellenbosch Central Analytical Facility (CAF). The synthesis and analytical data of compounds 1a–d have been described before [14 (link)].