Actin antibody c 11

Anti-mouse B220 (Clone RA3-6B2), CD43 (Clone eBioR2/60), IgM (Clone II/41), CD3 (Clone 145-2C11), CD4 (Clone RM4-5), CD8 (Clone 53-6.7) and 7-amino-actinomycin D (7-AAD) were obtained from eBioscience (San Diego, CA, USA). Anti-mouse IgG1 (Clone A85-1), αβTCR, Streptavidin Particles Plus-DM, and Annexin V-FITC were from BD Biosciences (San Jose, CA, USA). APLF antibody (N-16) and actin antibody (C-11) were from Santa Cruz (Dallas, TX, USA). Recombinant murine interleukin 4 (IL-4), IL-3, IL-6, Flt3-ligand, stem cell factor (SCF), TPO, and recombinant human TSLP were from Peprotech (Rocky Hill, NJ, USA), lipopolysaccharides (LPS) from Sigma (Oakville, ON, Canada), propidium iodide (PI) from Invitrogen (Waltham, MA, USA), and red blood cell lysing buffer from Sigma.

ERα Antibody (F-10) (sc-8002, Santa Cruz Biotechnology, Dallas, TX, USA), Anti-HA-tag mAb (M180-3, MBL, Nagoya, Japan), Monoclonal ANTI-FLAG M2 antibody produced in mouse (F1804, Merck Millipore), Ran BP-1 Antibody (M-45) (sc-28576, Santa Cruz Biotechnology), Actin Antibody (C-11) (sc-1615, Santa Cruz Biotechnology), Purified Mouse Anti-Karyopherin β (610559, BD Transduction Laboratories, San Jose, CA, USA), Purified Mouse anti-Transportin (558660, BD Transduction Laboratories), TNPO2 Polyclonal antibody (17831-1-AP, ProteinTech, Chicago, IL, USA), IPO4 Rabbit pAb (A15600, ABclonal, Cambridge, MA, USA), Anti-Importin 7 antibody (ab99273, abcam, Cambridge, MA, USA), Anti-Cellular Apoptosis Susceptibility/CSE1L antibody (ab96755, abcam), Purified Mouse Anti-Ran (610340, BD Transduction Laboratories), Phospho-Akt (Ser473) (D9E) XP Rabbit mAb (4060, Cell Signaling Technology, Danvers, MA, USA), Akt (pan) (C67E7) Rabbit mAb (4691, Cell Signaling Technology) were used.