Alprenolol

Manufactured by Bio-Techne

Alprenolol is a laboratory reagent used in various research applications. It is a beta-adrenergic antagonist that can be used to study the effects of beta-adrenergic receptor activity in cell-based models. The core function of Alprenolol is to selectively bind to and block beta-adrenergic receptors, allowing researchers to investigate the role of these receptors in biological processes.

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3 protocols using alprenolol

Modulation of HEK293 Cell Signaling

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Human HEK293 and HEK293T cells (ATCC, CRL-1573 and 3216) were grown in DMEM (Gibco/Thermo Fisher, Waltham, MA J; 11965) supplemented with 10% fetal bovine serum (UCSF Cell Culture Facility, San Francisco, CA), and maintained at 37°C and 5% CO₂ in a humidified incubator. Cells were treated with the following ligands and inhibitor: isoproterenol (Iso; Sigma, St. Louis MO; 6504), alprenolol, (Alp; Tocris, Minneapolis, MN; 2806), CGP-12177 (CGP; Tocris,1134), forskolin (Fsk; Sigma, F6886), isobutylmethylxanthine (IBMX; Sigma, I5879), and Dyngo4a (abcam, Cambridge, MA; ab120689). Prior to Dyngo4a treatment, cells were changed to serum-free media (DMEM without added FBS).

Peng G.E., Pessino V., Huang B, & von Zastrow M. (2021). Spatial decoding of endosomal cAMP signals by a metastable cytoplasmic PKA network. Nature chemical biology, 17(5), 558-566.

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Screening of Small Molecule Modulators

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Acebutolol, alprenolol, atenolol, bucindolol, carazolol, carvedilol, CGP12177, ICI 118551, isoproterenol, labetalol, metoprolol, nadolol, nebivolol, pronethalol, timolol, and 2-{[β-(4-Hydroxyphenyl)ethyl]aminomethyl}-1-tetralone hydrochloride (HEAT HCl) were purchased from Tocris (Bristol, United Kindom). Propranolol HCl and 4-hydroxycarbazole were obtained from Sigma-Aldrich (St. Louis, MO) and bupranolol was obtained from Abcam (Cambridge, UK). All compounds were dissolved in DMSO to obtain a 10 mM stock concentration, which was stored at -20°C. EGF was purchased from Peprotech (Rocky Hill, NJ) and dissolved in sterile deionized water at a 10 ug/mL stock and stored at -80°C. Primers were purchased from IDT (Coralville, IA). The lentiviral vector pLV-H1-EF1α-puro, annealing buffer, packaging vector, and polybrene were purchased from Biosettia Inc. (San Diego, CA). 4-hydroxycarbazole (4-OHC) was purchased from Sigma Aldrich

Cleveland K.H., Liang S., Chang A., Huang K.M., Chen S., Guo L., Huang Y, & Andresen B.T. (2019). Carvedilol inhibits EGF-mediated JB6 P+ colony formation through a mechanism independent of adrenoceptors. PLoS ONE, 14(5), e0217038.

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Modulation of HEK293 Cell Signaling

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Peng G.E., Pessino V., Huang B, & von Zastrow M. (2021). Spatial decoding of endosomal cAMP signals by a metastable cytoplasmic PKA network. Nature chemical biology, 17(5), 558-566.

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