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Quantikine human fgf basic immunoassay

Manufactured by R&D Systems
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The Quantikine Human FGF Basic Immunoassay is a quantitative sandwich enzyme immunoassay designed to measure human basic fibroblast growth factor (FGF basic) levels in cell culture supernates, serum, and plasma. It employs the quantitative sandwich enzyme immunoassay technique.

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Lab products found in correlation

Quantikine rat vegf immunoassay, by R&D Systems (1 mentions) Human vegf elisa kit, by R&D Systems (1 mentions) Quantikine human il 6 immunoassay, by R&D Systems (1 mentions) Human fgf basic elisa kit, by R&D Systems (1 mentions) Human il 1β elisa kit, by R&D Systems (1 mentions)

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Basic FGF (37 citations) Quantikine Human Immunoassay (17 citations) Quantikine Immunoassays (15 citations) Human basic FGF (4 citations) Quantikines (4 citations) Human FGF (2 citations) Quantikine Human (2 citations)

3 protocols using quantikine human fgf basic immunoassay

1

IGF-1 Release Kinetics from A-PLO-G Microbeads

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Insulin-like growth factor 1 loaded A-PLO-G microbeads were placed in a 22 mmol/L CaCl2 solution (pH 7.2) and incubated on a rotating incubator at 37 °C. The buffer was replaced at 10 time points, i.e. 1, 6, 12, 24 and 36 h; and then at 2, 3, 4, 5, 8 and 14 days. The amount of IGF-1 released into the PBS was assessed by ELISA, performed according to the manufacturer’s instructions (Quantikine Human FGF Basic Immunoassay; R&D Systems, Minneapolis, MN, USA).
Yan H., Zhong L., Jiang Y., Yang J., Deng J., Wei S., Opara E., Atala A., Mao X., Damaser M.S, & Zhang Y. (2017). Controlled release of insulin-like growth factor 1 enhances urethral sphincter function and histological structure in the treatment of female stress urinary incontinence in a rat model. BJU international, 121(2), 301-312.
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2

Quantifying Kidney Growth Factors

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Growth factors were isolated from kidneys as described (13 (link)). Concentrations of bFGF and VEGF were determined with the Quantikine human FGF basic immunoassay (R&D Systems # DFB50) and Quantikine rat VEGF immunoassay (R&D Systems # RRV00). Assays were performed in duplicate for each of three kidneys per group. The amount of remaining growth factor was normalized to the initial dry-weight of control kidneys to allow for comparison across all conditions.
Caralt M., Uzarski J.S., Iacob S., Obergfell K.P., Berg N., Bijonowski B.M., Kiefer K.M., Ward H.H., Wandinger-Ness A., Miller W.M., Zhang Z.J., Abecassis M.M, & Wertheim J.A. (2014). Optimization and critical evaluation of decellularization strategies to develop renal extracellular matrix scaffolds as biological templates for organ engineering and transplantation. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 15(1), 64-75.
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3

Quantification of Growth Factors and Cytokines in Cryopreserved Allogeneic Limbal Epithelial Cells

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Cryopreserved allo-CEGs were thawed in water heated to 37 °C, and the cryopreserved solution was washed repeatedly with physiological saline to eliminate the freezing mixture. To each epithelial cell sheet (150 cm2), 2.0 mL of physiological saline was added. After solubilizing the cell sheets by ultrasonication and using a homogenizer, dilution to a total liquid volume of 2.5 mL was performed in a measuring cylinder and the resulting solution was used as the “solubilized solution.” Nine types of growth factors and cytokines (IL-1α, IL-1β, PDGF-AA, TGF-α, TGF-β1, VEGF, KGF, b-FGF, IL-6, and HGF) and MMP-1 were measured with ELISA kits.
[ELISA Kits Used]: Human IL-1α: Life Technologies (IL-1α ELISA Kit), human IL-1β: Life Technologies (Human IL-1β ELISA Kit), human/mouse PDGF-AA: R&D Systems (Quantikine® Human/Mouse PDGF-AA Immunoassay), human TGF-α: R&D Systems (Quantikine® Human TGF-α Immunoassay), human TGF-β1: R&D Systems (Quantikine® Human TGF-β1 Immunoassay), human VEGF: Life Technologies (Human VEGF ELISA Kit), human KGF: R&D Systems (Quantikine® Human KGF Immunoassay), Human b-FGF: Life Technologies (Human FGF Basic ELISA Kit): R&D Systems® (Quantikine® Human FGF basic Immunoassay), human IL-6: R&D Systems (Quantikine® Human IL-6 Immunoassay), human Pro-MMP-1: R&D Systems (Quantikine® ELISA Human Pro-MMP-1), human HGF: Life Technologies (Human HGF ELISA Kit).
Yanaga H., Udoh Y., Yamamoto M., Yoshii S., Mori S., Yamauchi T., Kiyokawa K., Koga M, & Yanaga K. (2017). Cryopreserved cultured epithelial allografts for pediatric deep partial dermal burns: Early wound closure and suppression of scarring. Regenerative Therapy, 6, 74-82.
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