Bovine aortic endothelial cells were purchased from TOYOBO (Osaka, Japan). Cells were cultured in Dulbecco's modified Eagle's medium (Wako, Osaka, Japan) with 10% fetal bovine serum and 1.0, 2.5, 4.0, 5.5 or 25 mmol/L glucose in an atmosphere containing 5% CO2 and 95% air at 37°C. Etomoxir, 1‐(2,3,4‐trimethoxybenzyl) piperazine dihydrochloride (trimetazidine), bis‐2‐(5‐phenylacetamido‐1,3,4‐thiadiazol‐2‐yl) ethyl sulfide and carbonyl cyanide 3‐chlorophenylhydrazone were from Sigma‐Aldrich (St. Louis, Missouri, USA).
Bis 2 5 phenylacetamido 1 3 4 thiadiazol 2 yl ethyl sulfide
Manufactured by Merck Group
Sourced in United States
Bis‐2‐(5‐phenylacetamido‐1,3,4‐thiadiazol‐2‐yl) ethyl sulfide is a chemical compound. It is a solid substance with a molecular formula of C₂₂H₂₂N₄O₂S₂.
Automatically generated - may contain errors
Lab products found in correlation
Carbonyl cyanide 3 chlorophenylhydrazone, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium, by Fujifilm (1 mentions)
Pyruvate, by Merck Group (1 mentions)
Alpha ketoglutarate, by Merck Group (1 mentions)
Bptes, by Merck Group (1 mentions)
Dimethyl α ketoglutarate, by Merck Group (1 mentions)
L γ glutamyl p nitroanilide gpna, by Merck Group (1 mentions)
Yoyo 1, by Thermo Fisher Scientific (1 mentions)
2 protocols using bis 2 5 phenylacetamido 1 3 4 thiadiazol 2 yl ethyl sulfide
1
Bovine Aortic Endothelial Cell Culture
2
Metabolic Profiling with Chemical Probes
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YOYO-1 and SytoGreen24 were diluted in DMSO and used at a final concentration of 100 nM and 50 nM respectively (Life Technologies). Dimethyl-α-ketoglutarate (alpha-ketoglutarate) and pyruvate were purchased from Sigma and used at 3.5 mM and 8 mM respectively. Bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl)ethyl sulfide, or BPTES (Sigma) was solubilized in DMSO, subsequently diluted in methanol and used at a final concentration of 2.5 μM. QVD-OPH (SMBiochemicals) and was dissolved in DMSO and used at a final concentration of 20 μM. L-γ-Glutamyl-p-nitroanilide (GPNA) (Sigma), was prepared in DMSO in a 1 M stock solution and used at a final concentration of 5mM.
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