The RNeasy micro and the omniscript reverse transcription kits (Qiagen) were used to extract RNA and generate cDNA respectively. QPCR TaqMan assays (ThermoFisher) used: Hs00256257_m1, (BCL11B), Hs00607336_gH (HSP90), Hs01060665_g1, (ACTB).
Qpcr taqman assays
Manufactured by Thermo Fisher Scientific
QPCR TaqMan assays are a type of quantitative polymerase chain reaction (qPCR) technology used for gene expression analysis. They utilize fluorescent probes to detect and quantify specific nucleic acid sequences during the amplification process.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using qpcr taqman assays
1
Transcriptional Analysis of BCL11B, HSP90, and ACTB
2
Genetic Profiling of Disease Biomarkers
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Briefly, all the genotyping assays were performed by qPCR Taqman Assays (Thermo Fisher Scientific) from genomic DNA previously collected from the patients. The SNPs analysed are summarised on Table 1 .
Summary of genomic information of the SNPs analysed in patients included the dataset
| SNP | Chromosome | Gene or region involved |
|---|---|---|
| rs10782665 | 1 | PTGFR |
| rs4072037 | 1 | MUC1 |
| rs12724719 | 1 | CRABP2 |
| rs6686438 | 1 | PTGFR |
| rs1801133 | 1 | MTHFR |
| rs1328441 | 1 | PTGFR |
| rs523349 | 2 | SRD5A2 |
| rs11126936 | 2 | SLC30A3 |
| rs13078881 | 3 | BTD |
| rs2282679 | 4 | DBP |
| rs921943 | 5 | DMGDH |
| rs6198 | 5 | GR |
| rs33972313 | 5 | SLC23A1 |
| rs39848 | 5 | SRD5A1 |
| rs13283456 | 9 | PTGES2 |
| rs533116 | 11 | PTGDR2 |
| rs545659 | 11 | PTGDR2 |
| rs964184 | 11 | ZPR1 |
| rs2229765 | 15 | IGF-1R |
| rs2470152 | 15 | CYP19A1 |
| rs1800566 | 16 | NQO1 |
| rs9282861 | 16 | SULT1A1 |
| rs1800012 | 17 | COL1A1 |
| rs4343 | 17 | ACE |
| rs602662 | 19 | FUT2 |
| rs855791 | 22 | TMPRSS6 |
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