Dmem f12 glutamax modified medium

Human Osteosarcoma cell lines MG63 (ATCC^® CRL1427™), SAOS-2 (ATCC^® HTB-85™), U2-OS (ATCC^® HTB-96™); Human Adenocarcinoma cell lines isolated from breast cancers MDA-MB-468 (ATCC^® HTB231™); and Human Glioblastoma cell lines U87 (ATCC^® HTB14™) were purchased from American Type Culture Collection (ATCC) and used for this study.
MG63 cell line was cultured in DMEM F12-GlutaMAX™ Modified Medium (Gibco) supplemented with 10% Foetal Bovine Serum (FBS) (Gibco) and 1% of penicillin/streptomycin mixture (pen/strep) (100 U/mL–100 μg/mL, Gibco). SAOS-2 cell line was cultured in McCoy’s 5A Modified Medium (Gibco) supplemented with 15% and 10% FBS, respectively, and 1% pen/strep. U2-OS cells were cultured using McCoy’s 5 A (modified) medium supplemented with 10% FBS and 1% Pen/Strep. MDA MB 468 cells were cultured in growth media using RPMI 1640 (Gibco), 10% FBS and 1% Pen/Strep; and U87 cells were grown in a complete medium composed of MEM-nucleosides no-ascorbic-acid medium (Gibco), 10% FBS and 1% Pen/Strep.
Cells were kept in an incubator at 37°C under controlled humidity and 5% CO₂ atmosphere conditions. Cells were detached from culture flasks by trypsinization and centrifuged. The cell number and viability were determined by Trypan Blue Dye Exclusion test and all cell handling procedures were performed under laminar flow hood in sterility conditions.

Human Osteosarcoma cell lines MG63 (ATCC^® CRL1427™), U-2OS (ATCC^® HTB-96™), and SAOS-2 (ATCC^® HTB-85™), purchased from American Type Culture Collection (ATCC), were used. MG63 cell line was cultured in DMEM F12-GlutaMAX™ Modified Medium (Gibco) supplemented with 10% Foetal Bovine Serum (FBS) (Gibco) and 1% of penicillin/streptomycin mixture (pen/strep) (100 U/ml—100 μg/ml, Gibco). SAOS-2 and U-2OS cell lines were cultured in McCoy’s 5A Modified Medium (Gibco) supplemented with 15 and 10% FBS, respectively, and 1% pen/strep. Cells were kept in an incubator at 37°C under controlled humidity and 5% CO₂ atmosphere conditions. Cells were detached from culture flasks by trypsinization and centrifuged. The cell number and viability were determined by Trypan Blue Dye Exclusion test and all cell handling procedures were performed under a laminar flow hood in sterility conditions. For the experiment, all cell lines were seeded 5.0 × 10³ cells/well in 96 well-plates and 5.0 × 10⁴ cells/well in 6 well-plates.